Specific serology for emerging human coronaviruses by protein microarray

Detalhes bibliográficos
Autor(a) principal: Reusken, C.
Data de Publicação: 2013
Outros Autores: Mou, H., Godeke, G. J., Hoek, L., Meyer, B., Müller, M. A., Haagmans, B., Sousa, R., Shuurman, N., Rottier, P., Osterhaus, A., Drosten, C., Bosch, B. J., Koopmans, M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/4034
Resumo: We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.
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spelling Specific serology for emerging human coronaviruses by protein microarrayEmerging Human CoronavirusesProtein MicroarraySpecific SerologyInfecções Sistémicas e ZoonosesWe present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.RdS was funded by the European Public Health Microbiology Training Program (EUPHEM), ECDC, Stockholm. LvdH, BH and AO were funded by the European Union FP7 project EMPERIE (contract number 223498).European Centre for Disease Prevention and ControlRepositório Científico do Instituto Nacional de SaúdeReusken, C.Mou, H.Godeke, G. J.Hoek, L.Meyer, B.Müller, M. A.Haagmans, B.Sousa, R.Shuurman, N.Rottier, P.Osterhaus, A.Drosten, C.Bosch, B. J.Koopmans, M.2016-10-06T13:58:07Z2013-04-042013-04-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/4034engEuro Surveill. 2013 Apr 4;18(14):204411560-791710.2807/1560-7917.ES2015.20.4.21023info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:55Zoai:repositorio.insa.pt:10400.18/4034Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:38:31.283377Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Specific serology for emerging human coronaviruses by protein microarray
title Specific serology for emerging human coronaviruses by protein microarray
spellingShingle Specific serology for emerging human coronaviruses by protein microarray
Reusken, C.
Emerging Human Coronaviruses
Protein Microarray
Specific Serology
Infecções Sistémicas e Zoonoses
title_short Specific serology for emerging human coronaviruses by protein microarray
title_full Specific serology for emerging human coronaviruses by protein microarray
title_fullStr Specific serology for emerging human coronaviruses by protein microarray
title_full_unstemmed Specific serology for emerging human coronaviruses by protein microarray
title_sort Specific serology for emerging human coronaviruses by protein microarray
author Reusken, C.
author_facet Reusken, C.
Mou, H.
Godeke, G. J.
Hoek, L.
Meyer, B.
Müller, M. A.
Haagmans, B.
Sousa, R.
Shuurman, N.
Rottier, P.
Osterhaus, A.
Drosten, C.
Bosch, B. J.
Koopmans, M.
author_role author
author2 Mou, H.
Godeke, G. J.
Hoek, L.
Meyer, B.
Müller, M. A.
Haagmans, B.
Sousa, R.
Shuurman, N.
Rottier, P.
Osterhaus, A.
Drosten, C.
Bosch, B. J.
Koopmans, M.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Reusken, C.
Mou, H.
Godeke, G. J.
Hoek, L.
Meyer, B.
Müller, M. A.
Haagmans, B.
Sousa, R.
Shuurman, N.
Rottier, P.
Osterhaus, A.
Drosten, C.
Bosch, B. J.
Koopmans, M.
dc.subject.por.fl_str_mv Emerging Human Coronaviruses
Protein Microarray
Specific Serology
Infecções Sistémicas e Zoonoses
topic Emerging Human Coronaviruses
Protein Microarray
Specific Serology
Infecções Sistémicas e Zoonoses
description We present a serological assay for the specific detection of IgM and IgG antibodies against the emerging human coronavirus hCoV-EMC and the SARS-CoV based on protein microarray technology. The assay uses the S1 receptor-binding subunit of the spike protein of hCoV-EMC and SARS-CoV as antigens. The assay has been validated extensively using putative cross-reacting sera of patient cohorts exposed to the four common hCoVs and sera from convalescent patients infected with hCoV-EMC or SARS-CoV.
publishDate 2013
dc.date.none.fl_str_mv 2013-04-04
2013-04-04T00:00:00Z
2016-10-06T13:58:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/4034
url http://hdl.handle.net/10400.18/4034
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Euro Surveill. 2013 Apr 4;18(14):20441
1560-7917
10.2807/1560-7917.ES2015.20.4.21023
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv European Centre for Disease Prevention and Control
publisher.none.fl_str_mv European Centre for Disease Prevention and Control
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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