Comprehensive identification of meningococcal genes and small noncoding RNAs required for host cell colonization
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10451/53378 |
Resumo: | © 2016 Capel et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
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Comprehensive identification of meningococcal genes and small noncoding RNAs required for host cell colonization© 2016 Capel et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.Neisseria meningitidis is a leading cause of bacterial meningitis and septicemia, affecting infants and adults worldwide. N. meningitidis is also a common inhabitant of the human nasopharynx and, as such, is highly adapted to its niche. During bacteremia, N. meningitidis gains access to the blood compartment, where it adheres to endothelial cells of blood vessels and causes dramatic vascular damage. Colonization of the nasopharyngeal niche and communication with the different human cell types is a major issue of the N. meningitidis life cycle that is poorly understood. Here, highly saturated random transposon insertion libraries of N. meningitidis were engineered, and the fitness of mutations during routine growth and that of colonization of endothelial and epithelial cells in a flow device were assessed in a transposon insertion site sequencing (Tn-seq) analysis. This allowed the identification of genes essential for bacterial growth and genes specifically required for host cell colonization. In addition, after having identified the small noncoding RNAs (sRNAs) located in intergenic regions, the phenotypes associated with mutations in those sRNAs were defined. A total of 383 genes and 8 intergenic regions containing sRNA candidates were identified to be essential for growth, while 288 genes and 33 intergenic regions containing sRNA candidates were found to be specifically required for host cell colonization. Importance: Meningococcal meningitis is a common cause of meningitis in infants and adults. Neisseria meningitidis (meningococcus) is also a commensal bacterium of the nasopharynx and is carried by 3 to 30% of healthy humans. Under some unknown circumstances, N. meningitidis is able to invade the bloodstream and cause either meningitis or a fatal septicemia known as purpura fulminans. The onset of symptoms is sudden, and death can follow within hours. Although many meningococcal virulence factors have been identified, the mechanisms that allow the bacterium to switch from the commensal to pathogen state remain unknown. Therefore, we used a Tn-seq strategy coupled to high-throughput DNA sequencing technologies to find genes for proteins used by N. meningitidis to specifically colonize epithelial cells and primary brain endothelial cells. We identified 383 genes and 8 intergenic regions containing sRNAs essential for growth and 288 genes and 33 intergenic regions containing sRNAs required specifically for host cell colonization.This work was supported by ANR grant ANR-14-IFEC-0006-01 call ERANET INFECT-ERA 2014, the grant program EMERGENCE from La Mairie de Paris, and a postdoctoral grant supported by the DIM Malinf from the Conseil Régional de l’Ile-De-France. The laboratory of X.N. is supported by INSERM, CNRS, Université Paris Descartes, and the Fondation pour la Recherche Médicale.ASM JournalsRepositório da Universidade de LisboaCapel, ElenaZomer, Aldert L.Nussbaumer, ThomasBole, ChristineIzac, BrigitteFrapy, EricMeyer, JulieBouzinba-Ségard, HaniaaBille, EmmanuelleJamet, AnneCavau, AnneLetourneur, FranckBourdoulous, SandrineRattei, ThomasNassif, XavierCoureuil, MathieuSeifert, H.Shafer, William2022-06-14T15:26:39Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/53378engmBio. 2016 Aug 2;7(4):e01173-162161-212910.1128/mBio.01173-162150-7511info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:59:05Zoai:repositorio.ul.pt:10451/53378Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:04:19.081405Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Comprehensive identification of meningococcal genes and small noncoding RNAs required for host cell colonization |
| title |
Comprehensive identification of meningococcal genes and small noncoding RNAs required for host cell colonization |
| spellingShingle |
Comprehensive identification of meningococcal genes and small noncoding RNAs required for host cell colonization Capel, Elena |
| title_short |
Comprehensive identification of meningococcal genes and small noncoding RNAs required for host cell colonization |
| title_full |
Comprehensive identification of meningococcal genes and small noncoding RNAs required for host cell colonization |
| title_fullStr |
Comprehensive identification of meningococcal genes and small noncoding RNAs required for host cell colonization |
| title_full_unstemmed |
Comprehensive identification of meningococcal genes and small noncoding RNAs required for host cell colonization |
| title_sort |
Comprehensive identification of meningococcal genes and small noncoding RNAs required for host cell colonization |
| author |
Capel, Elena |
| author_facet |
Capel, Elena Zomer, Aldert L. Nussbaumer, Thomas Bole, Christine Izac, Brigitte Frapy, Eric Meyer, Julie Bouzinba-Ségard, Haniaa Bille, Emmanuelle Jamet, Anne Cavau, Anne Letourneur, Franck Bourdoulous, Sandrine Rattei, Thomas Nassif, Xavier Coureuil, Mathieu Seifert, H. Shafer, William |
| author_role |
author |
| author2 |
Zomer, Aldert L. Nussbaumer, Thomas Bole, Christine Izac, Brigitte Frapy, Eric Meyer, Julie Bouzinba-Ségard, Haniaa Bille, Emmanuelle Jamet, Anne Cavau, Anne Letourneur, Franck Bourdoulous, Sandrine Rattei, Thomas Nassif, Xavier Coureuil, Mathieu Seifert, H. Shafer, William |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Capel, Elena Zomer, Aldert L. Nussbaumer, Thomas Bole, Christine Izac, Brigitte Frapy, Eric Meyer, Julie Bouzinba-Ségard, Haniaa Bille, Emmanuelle Jamet, Anne Cavau, Anne Letourneur, Franck Bourdoulous, Sandrine Rattei, Thomas Nassif, Xavier Coureuil, Mathieu Seifert, H. Shafer, William |
| description |
© 2016 Capel et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z 2022-06-14T15:26:39Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/53378 |
| url |
http://hdl.handle.net/10451/53378 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
mBio. 2016 Aug 2;7(4):e01173-16 2161-2129 10.1128/mBio.01173-16 2150-7511 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
ASM Journals |
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ASM Journals |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134594063138816 |