Plasma is the main regulator of Staphylococcus epidermidis biofilms virulence genes transcription in human blood
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/43243 |
Resumo: | Staphylococcus epidermidis is frequently associated with the emergence of medical device- associated bloodstream infections, due to its ability to form biofilms on the surface of vascular catheters. Although these biofilms may be in continuous contact with human blood, how S. epidermidis biofilm cells interact with blood and its cellular and soluble components is poorly understood. Herein, we evaluated biofilm structure, biofilm cells culturability and viability, and the transcription of a panel of genes associated with S. epidermidis biofilms virulence, upon interaction with whole human blood or plasma. Our results showed that although whole human blood caused significant alterations in biofilm structure and in the number of culturable and viable cells, plasma was the main regulator of the transcription of genes with central role in biofilm formation, maturation and immune evasion. These findings highlight the urgent need to intensify studies aiming to evaluate the impact of host soluble factors on S. epidermidis biofilms fitness and persistence. |
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Plasma is the main regulator of Staphylococcus epidermidis biofilms virulence genes transcription in human bloodStaphylococcus epidermidisbiofilmshuman bloodplasmavirulence genesgene expressionScience & TechnologyStaphylococcus epidermidis is frequently associated with the emergence of medical device- associated bloodstream infections, due to its ability to form biofilms on the surface of vascular catheters. Although these biofilms may be in continuous contact with human blood, how S. epidermidis biofilm cells interact with blood and its cellular and soluble components is poorly understood. Herein, we evaluated biofilm structure, biofilm cells culturability and viability, and the transcription of a panel of genes associated with S. epidermidis biofilms virulence, upon interaction with whole human blood or plasma. Our results showed that although whole human blood caused significant alterations in biofilm structure and in the number of culturable and viable cells, plasma was the main regulator of the transcription of genes with central role in biofilm formation, maturation and immune evasion. These findings highlight the urgent need to intensify studies aiming to evaluate the impact of host soluble factors on S. epidermidis biofilms fitness and persistence.This work was supported by European Union funds (FEDER/COMPETE) and by national funds (FCT) under the project with reference FCOMP-01-0124-FEDER-041246 (EXPL/BIAMIC/0101/2013). The authors thank the FCT Strategic Project of UID/BIO/04469/2013 unit and the project FCOMP-01-0124-FEDER-027462 (RECI/BBB-EBI/0179/2012). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. AF acknowledges the financial support of an individual grant SFRH/BPD/99961/2014. NC is an Investigador FCT. The authors would like to thank Sandra Maia and Filomena Costa for human blood collection procedure. The authors declare no conflict of interest.WileyUniversidade do MinhoFrança, Ângela Maria Oliveira SousaCerca, Nuno2016-032016-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/43243engFrança, Angela; Cerca, Nuno, Plasma is the main regulator of Staphylococcus epidermidis biofilms virulence genes transcription in human blood. Pathogens and Disease, 74(2), ftv125, 20162049-632X2049-632X10.1093/femspd/ftv12526712650http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)2049-632Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:03:19Zoai:repositorium.sdum.uminho.pt:1822/43243Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:53:25.547288Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Plasma is the main regulator of Staphylococcus epidermidis biofilms virulence genes transcription in human blood |
title |
Plasma is the main regulator of Staphylococcus epidermidis biofilms virulence genes transcription in human blood |
spellingShingle |
Plasma is the main regulator of Staphylococcus epidermidis biofilms virulence genes transcription in human blood França, Ângela Maria Oliveira Sousa Staphylococcus epidermidis biofilms human blood plasma virulence genes gene expression Science & Technology |
title_short |
Plasma is the main regulator of Staphylococcus epidermidis biofilms virulence genes transcription in human blood |
title_full |
Plasma is the main regulator of Staphylococcus epidermidis biofilms virulence genes transcription in human blood |
title_fullStr |
Plasma is the main regulator of Staphylococcus epidermidis biofilms virulence genes transcription in human blood |
title_full_unstemmed |
Plasma is the main regulator of Staphylococcus epidermidis biofilms virulence genes transcription in human blood |
title_sort |
Plasma is the main regulator of Staphylococcus epidermidis biofilms virulence genes transcription in human blood |
author |
França, Ângela Maria Oliveira Sousa |
author_facet |
França, Ângela Maria Oliveira Sousa Cerca, Nuno |
author_role |
author |
author2 |
Cerca, Nuno |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
França, Ângela Maria Oliveira Sousa Cerca, Nuno |
dc.subject.por.fl_str_mv |
Staphylococcus epidermidis biofilms human blood plasma virulence genes gene expression Science & Technology |
topic |
Staphylococcus epidermidis biofilms human blood plasma virulence genes gene expression Science & Technology |
description |
Staphylococcus epidermidis is frequently associated with the emergence of medical device- associated bloodstream infections, due to its ability to form biofilms on the surface of vascular catheters. Although these biofilms may be in continuous contact with human blood, how S. epidermidis biofilm cells interact with blood and its cellular and soluble components is poorly understood. Herein, we evaluated biofilm structure, biofilm cells culturability and viability, and the transcription of a panel of genes associated with S. epidermidis biofilms virulence, upon interaction with whole human blood or plasma. Our results showed that although whole human blood caused significant alterations in biofilm structure and in the number of culturable and viable cells, plasma was the main regulator of the transcription of genes with central role in biofilm formation, maturation and immune evasion. These findings highlight the urgent need to intensify studies aiming to evaluate the impact of host soluble factors on S. epidermidis biofilms fitness and persistence. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03 2016-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/43243 |
url |
http://hdl.handle.net/1822/43243 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
França, Angela; Cerca, Nuno, Plasma is the main regulator of Staphylococcus epidermidis biofilms virulence genes transcription in human blood. Pathogens and Disease, 74(2), ftv125, 2016 2049-632X 2049-632X 10.1093/femspd/ftv125 26712650 http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)2049-632X |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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