Interaction of Moringa oleifera seed lectin with humic acid
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/16716 |
Resumo: | The aim of this work was to characterise the affinity of protein preparations from Moringa oleifera seeds, specifically extract (seeds homogenised with 0.15 M NaCl), fraction (extract precipitated with 390 mg mL−1 of ammonium sulphate) and cMoL (coagulant M. oleifera lectin) to bind humic acids using a haemagglutinating activity assay with rabbit erythrocytes and a radial diffusion assay in agarose gel. Specific haemagglutinating activity (SHA) decreased by 94 % for the extract and cMoL and by 50 % for the fraction in the presence of humic acid. Precipitation bands were observed in the diffusion gel. Both results suggested humic acid-cMoL binding. Carbohydrates, potassium, and calcium ions and pH affected the SHA of cMoL. As an example of application, cMoL was immobilised on a column packed with sepharose receiving 20 mg mL−1 of carbon humic acid solution, 30 mg of humic acid per gram of support was removed. This result suggested that protein preparations might be used in water treatment to remove humic acids. |
id |
RCAP_73f79bdccf1b07a3066a3381868add12 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/16716 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Interaction of Moringa oleifera seed lectin with humic acidHumic acidLectinMoringa oleiferaProtein preparationsWater treatmentScience & TechnologyThe aim of this work was to characterise the affinity of protein preparations from Moringa oleifera seeds, specifically extract (seeds homogenised with 0.15 M NaCl), fraction (extract precipitated with 390 mg mL−1 of ammonium sulphate) and cMoL (coagulant M. oleifera lectin) to bind humic acids using a haemagglutinating activity assay with rabbit erythrocytes and a radial diffusion assay in agarose gel. Specific haemagglutinating activity (SHA) decreased by 94 % for the extract and cMoL and by 50 % for the fraction in the presence of humic acid. Precipitation bands were observed in the diffusion gel. Both results suggested humic acid-cMoL binding. Carbohydrates, potassium, and calcium ions and pH affected the SHA of cMoL. As an example of application, cMoL was immobilised on a column packed with sepharose receiving 20 mg mL−1 of carbon humic acid solution, 30 mg of humic acid per gram of support was removed. This result suggested that protein preparations might be used in water treatment to remove humic acids.The authors express their gratitude to the Fundacao para Ciencia e a Tecnologia (FCT), the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), the VALNATURA ALFA Programme and the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES) for their financial support.VersitaUniversidade do MinhoSantos, Andréa F. S.Carneiro-da-Cunha, Maria G.Teixeira, J. A.Paiva, Patrícia M. G.Coelho, L. C. B. B.Nogueira, R.2011-07-042011-07-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/16716eng0366-635210.2478/s11696-011-0025-2info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:42:19Zoai:repositorium.sdum.uminho.pt:1822/16716Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:39:32.410538Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Interaction of Moringa oleifera seed lectin with humic acid |
title |
Interaction of Moringa oleifera seed lectin with humic acid |
spellingShingle |
Interaction of Moringa oleifera seed lectin with humic acid Santos, Andréa F. S. Humic acid Lectin Moringa oleifera Protein preparations Water treatment Science & Technology |
title_short |
Interaction of Moringa oleifera seed lectin with humic acid |
title_full |
Interaction of Moringa oleifera seed lectin with humic acid |
title_fullStr |
Interaction of Moringa oleifera seed lectin with humic acid |
title_full_unstemmed |
Interaction of Moringa oleifera seed lectin with humic acid |
title_sort |
Interaction of Moringa oleifera seed lectin with humic acid |
author |
Santos, Andréa F. S. |
author_facet |
Santos, Andréa F. S. Carneiro-da-Cunha, Maria G. Teixeira, J. A. Paiva, Patrícia M. G. Coelho, L. C. B. B. Nogueira, R. |
author_role |
author |
author2 |
Carneiro-da-Cunha, Maria G. Teixeira, J. A. Paiva, Patrícia M. G. Coelho, L. C. B. B. Nogueira, R. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Santos, Andréa F. S. Carneiro-da-Cunha, Maria G. Teixeira, J. A. Paiva, Patrícia M. G. Coelho, L. C. B. B. Nogueira, R. |
dc.subject.por.fl_str_mv |
Humic acid Lectin Moringa oleifera Protein preparations Water treatment Science & Technology |
topic |
Humic acid Lectin Moringa oleifera Protein preparations Water treatment Science & Technology |
description |
The aim of this work was to characterise the affinity of protein preparations from Moringa oleifera seeds, specifically extract (seeds homogenised with 0.15 M NaCl), fraction (extract precipitated with 390 mg mL−1 of ammonium sulphate) and cMoL (coagulant M. oleifera lectin) to bind humic acids using a haemagglutinating activity assay with rabbit erythrocytes and a radial diffusion assay in agarose gel. Specific haemagglutinating activity (SHA) decreased by 94 % for the extract and cMoL and by 50 % for the fraction in the presence of humic acid. Precipitation bands were observed in the diffusion gel. Both results suggested humic acid-cMoL binding. Carbohydrates, potassium, and calcium ions and pH affected the SHA of cMoL. As an example of application, cMoL was immobilised on a column packed with sepharose receiving 20 mg mL−1 of carbon humic acid solution, 30 mg of humic acid per gram of support was removed. This result suggested that protein preparations might be used in water treatment to remove humic acids. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-07-04 2011-07-04T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/16716 |
url |
http://hdl.handle.net/1822/16716 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0366-6352 10.2478/s11696-011-0025-2 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Versita |
publisher.none.fl_str_mv |
Versita |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132936948154368 |