Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer

Detalhes bibliográficos
Autor(a) principal: Tavares, Barbara
Data de Publicação: 2017
Outros Autores: Jacinto, Raquel, Sampaio, Pedro, Pestana, Sara, Pinto, Andreia, Vaz, Andreia, Roxo-Rosa, Mónica, Gardner, Rui, Lopes, Telma, Schilling, Britta, Henry, Ian, Saúde, Leonor, Lopes, Susana Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/54375
Resumo: © 2017, Tavares et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
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spelling Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizerNotch signallingCilia motilityCiliary epitheliumDevelopmental biologyFluid flow dynamicsHer12Left-rightStem cellsZebrafish© 2017, Tavares et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.Foxj1a is necessary and sufficient to specify motile cilia. Using transcriptional studies and slow-scan two-photon live imaging capable of identifying the number of motile and immotile cilia, we now established that the final number of motile cilia depends on Notch signalling (NS). We found that despite all left-right organizer (LRO) cells express foxj1a and the ciliary axonemes of these cells have dynein arms, some cilia remain immotile. We identified that this decision is taken early in development in the Kupffer's Vesicle (KV) precursors the readout being her12 transcription. We demonstrate that overexpression of either her12 or Notch intracellular domain (NICD) increases the number of immotile cilia at the expense of motile cilia, and leads to an accumulation of immotile cilia at the anterior half of the KV. This disrupts the normal fluid flow intensity and pattern, with consequent impact on dand5 expression pattern and left-right (L-R) axis establishment.eLife Sciences PublicationsRepositório da Universidade de LisboaTavares, BarbaraJacinto, RaquelSampaio, PedroPestana, SaraPinto, AndreiaVaz, AndreiaRoxo-Rosa, MónicaGardner, RuiLopes, TelmaSchilling, BrittaHenry, IanSaúde, LeonorLopes, Susana Santos2022-09-08T13:03:46Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/54375engElife. 2017 Sep 6;6:e2516510.7554/eLife.251652050-084Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:00:11Zoai:repositorio.ul.pt:10451/54375Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:04:54.957321Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer
title Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer
spellingShingle Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer
Tavares, Barbara
Notch signalling
Cilia motility
Ciliary epithelium
Developmental biology
Fluid flow dynamics
Her12
Left-right
Stem cells
Zebrafish
title_short Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer
title_full Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer
title_fullStr Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer
title_full_unstemmed Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer
title_sort Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer
author Tavares, Barbara
author_facet Tavares, Barbara
Jacinto, Raquel
Sampaio, Pedro
Pestana, Sara
Pinto, Andreia
Vaz, Andreia
Roxo-Rosa, Mónica
Gardner, Rui
Lopes, Telma
Schilling, Britta
Henry, Ian
Saúde, Leonor
Lopes, Susana Santos
author_role author
author2 Jacinto, Raquel
Sampaio, Pedro
Pestana, Sara
Pinto, Andreia
Vaz, Andreia
Roxo-Rosa, Mónica
Gardner, Rui
Lopes, Telma
Schilling, Britta
Henry, Ian
Saúde, Leonor
Lopes, Susana Santos
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Tavares, Barbara
Jacinto, Raquel
Sampaio, Pedro
Pestana, Sara
Pinto, Andreia
Vaz, Andreia
Roxo-Rosa, Mónica
Gardner, Rui
Lopes, Telma
Schilling, Britta
Henry, Ian
Saúde, Leonor
Lopes, Susana Santos
dc.subject.por.fl_str_mv Notch signalling
Cilia motility
Ciliary epithelium
Developmental biology
Fluid flow dynamics
Her12
Left-right
Stem cells
Zebrafish
topic Notch signalling
Cilia motility
Ciliary epithelium
Developmental biology
Fluid flow dynamics
Her12
Left-right
Stem cells
Zebrafish
description © 2017, Tavares et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2022-09-08T13:03:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/54375
url http://hdl.handle.net/10451/54375
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Elife. 2017 Sep 6;6:e25165
10.7554/eLife.25165
2050-084X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv eLife Sciences Publications
publisher.none.fl_str_mv eLife Sciences Publications
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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