Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer

Detalhes bibliográficos
Autor(a) principal: Tavares, Barbara
Data de Publicação: 2017
Outros Autores: Jacinto, Raquel, Sampaio, Pedro, Pestana, Sara, Pinto, Andreia, Vaz, Andreia, Roxo-Rosa, Mónica, Gardner, Rui, Lopes, Telma, Schilling, Britta, Henry, Ian, Saúde, Leonor, Lopes, Susana Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/151590
Resumo: undacao para a Ciencia e a Tecnologia PTDC/BEX-BID/1411/2014 Susana Santos Lopes Fundacao para a Ciencia e a Tecnologia FCT-ANR/BEX-BID/0153/2012 Sara Pestana Fundacao para a Ciencia e a Tecnologia PTDC/SAU-OBD/103981/2008 Andreia Vaz Fundacao para a Ciencia e a Tecnologia PD/BD/52420/2013 Raquel Jacinto Fundacao para a Ciencia e a Tecnologia SFRH/BPD/77258/2011 Barbara Tavares Fundacao para a Ciencia e a Tecnologia SFRH/BD/111611/2015 Pedro Sampaio Fundacao para a Ciencia e a Tecnologia IF/00951/2012 Susana Santos Lopes The funders had no role in study design, data collection and interpretation, or the on the decision to submit the work for publication.
id RCAP_afe71d922e82c4a47bccfbd068a09276
oai_identifier_str oai:run.unl.pt:10362/151590
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizerNeuroscience(all)Biochemistry, Genetics and Molecular Biology(all)Immunology and Microbiology(all)undacao para a Ciencia e a Tecnologia PTDC/BEX-BID/1411/2014 Susana Santos Lopes Fundacao para a Ciencia e a Tecnologia FCT-ANR/BEX-BID/0153/2012 Sara Pestana Fundacao para a Ciencia e a Tecnologia PTDC/SAU-OBD/103981/2008 Andreia Vaz Fundacao para a Ciencia e a Tecnologia PD/BD/52420/2013 Raquel Jacinto Fundacao para a Ciencia e a Tecnologia SFRH/BPD/77258/2011 Barbara Tavares Fundacao para a Ciencia e a Tecnologia SFRH/BD/111611/2015 Pedro Sampaio Fundacao para a Ciencia e a Tecnologia IF/00951/2012 Susana Santos Lopes The funders had no role in study design, data collection and interpretation, or the on the decision to submit the work for publication.Foxj1a is necessary and sufficient to specify motile cilia. Using transcriptional studies and slow-scan two-photon live imaging capable of identifying the number of motile and immotile cilia, we now established that the final number of motile cilia depends on Notch signalling (NS). We found that despite all left-right organizer (LRO) cells express foxj1a and the ciliary axonemes of these cells have dynein arms, some cilia remain immotile. We identified that this decision is taken early in development in the Kupffer’s Vesicle (KV) precursors the readout being her12 transcription. We demonstrate that overexpression of either her12 or Notch intracellular domain (NICD) increases the number of immotile cilia at the expense of motile cilia, and leads to an accumulation of immotile cilia at the anterior half of the KV. This disrupts the normal fluid flow intensity and pattern, with consequent impact on dand5 expression pattern and left-right (L-R) axis establishment.Centro de Estudos de Doenças Crónicas (CEDOC)NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNTavares, BarbaraJacinto, RaquelSampaio, PedroPestana, SaraPinto, AndreiaVaz, AndreiaRoxo-Rosa, MónicaGardner, RuiLopes, TelmaSchilling, BrittaHenry, IanSaúde, LeonorLopes, Susana Santos2023-04-04T22:18:58Z2017-09-062017-09-06T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/151590eng2050-084XPURE: 57651778https://doi.org/10.7554/eLife.25165info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:34:02Zoai:run.unl.pt:10362/151590Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:54:39.090144Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer
title Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer
spellingShingle Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer
Tavares, Barbara
Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
title_short Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer
title_full Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer
title_fullStr Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer
title_full_unstemmed Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer
title_sort Notch/Her12 signalling modulates, motile/immotile cilia ratio downstream of Foxj1a in zebrafish left-right organizer
author Tavares, Barbara
author_facet Tavares, Barbara
Jacinto, Raquel
Sampaio, Pedro
Pestana, Sara
Pinto, Andreia
Vaz, Andreia
Roxo-Rosa, Mónica
Gardner, Rui
Lopes, Telma
Schilling, Britta
Henry, Ian
Saúde, Leonor
Lopes, Susana Santos
author_role author
author2 Jacinto, Raquel
Sampaio, Pedro
Pestana, Sara
Pinto, Andreia
Vaz, Andreia
Roxo-Rosa, Mónica
Gardner, Rui
Lopes, Telma
Schilling, Britta
Henry, Ian
Saúde, Leonor
Lopes, Susana Santos
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Centro de Estudos de Doenças Crónicas (CEDOC)
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Tavares, Barbara
Jacinto, Raquel
Sampaio, Pedro
Pestana, Sara
Pinto, Andreia
Vaz, Andreia
Roxo-Rosa, Mónica
Gardner, Rui
Lopes, Telma
Schilling, Britta
Henry, Ian
Saúde, Leonor
Lopes, Susana Santos
dc.subject.por.fl_str_mv Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
topic Neuroscience(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)
description undacao para a Ciencia e a Tecnologia PTDC/BEX-BID/1411/2014 Susana Santos Lopes Fundacao para a Ciencia e a Tecnologia FCT-ANR/BEX-BID/0153/2012 Sara Pestana Fundacao para a Ciencia e a Tecnologia PTDC/SAU-OBD/103981/2008 Andreia Vaz Fundacao para a Ciencia e a Tecnologia PD/BD/52420/2013 Raquel Jacinto Fundacao para a Ciencia e a Tecnologia SFRH/BPD/77258/2011 Barbara Tavares Fundacao para a Ciencia e a Tecnologia SFRH/BD/111611/2015 Pedro Sampaio Fundacao para a Ciencia e a Tecnologia IF/00951/2012 Susana Santos Lopes The funders had no role in study design, data collection and interpretation, or the on the decision to submit the work for publication.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-06
2017-09-06T00:00:00Z
2023-04-04T22:18:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/151590
url http://hdl.handle.net/10362/151590
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2050-084X
PURE: 57651778
https://doi.org/10.7554/eLife.25165
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799138135264722944

Registros relacionados