Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers

Detalhes bibliográficos
Autor(a) principal: Serro, A. P.
Data de Publicação: 2014
Outros Autores: Galante, R., Kozica, A., Paradiso, P., Gonçalves da Silva, A.M.P.S., Luzyanina, K. V., Fernandes, A. C., Saramago, B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.26/8076
Resumo: “NOTICE: this is the author’s version of a work that was accepted for publication in Colloids and Surfaces B: Biointerfaces. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Colloids and Surfaces B: Biointerfaces, [Vol. 16, (April 2014)] DOI 10.1016/j.colsurfb.2013.12.042 "
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spelling Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayersTetracaineBiomembrane modelsLiposomesLangmuir monolayersDMPCCholesterol“NOTICE: this is the author’s version of a work that was accepted for publication in Colloids and Surfaces B: Biointerfaces. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Colloids and Surfaces B: Biointerfaces, [Vol. 16, (April 2014)] DOI 10.1016/j.colsurfb.2013.12.042 ""Different types of lipid bilayers/monolayers have been used to simulate the cellular membranes in the investigation of the interactions between drugs and cells. However, to our knowledge, very few studies focused on the influence of the chosen membrane model upon the obtained results. The main objective of this work is to understand how do the nature and immobilization state of the biomembrane models influence the effect of the local anaesthetic tetracaine (TTC) upon the lipid membranes. The interaction of TTC with different biomembrane models of dimyristoylphosphatidylcholine (DMPC) with and without cholesterol (CHOL) was investigated through several techniques. A quartz crystal microbalance with dissipation (QCM-D) was used to study the effect on immobilized liposomes, while phosphorus nuclear magnetic resonance (31P-NMR) and differential scanning calorimetry (DSC) were applied to liposomes in suspension. The effect of TTC on Langmuir monolayers of lipids was also investigated through surface pressure-area measurements at the air-water interface. The general conclusion was that TTC has a fluidizing effect on the lipid membranes and, above certain concentrations, induced membrane swelling or even solubilization. However, different models led to variable responses to the TTC action. The intensity of the disordering effect caused by TTC increased in the following order: supported liposomes < liposomes in solution < Langmuir monolayers. This means that extrapolation of the results obtain in in vitro studies of the lipid/anaesthetic interactions to in vivo conditions should be done carefully."ElsevierRepositório ComumSerro, A. P.Galante, R.Kozica, A.Paradiso, P.Gonçalves da Silva, A.M.P.S.Luzyanina, K. V.Fernandes, A. C.Saramago, B.2015-03-20T09:08:31Z2014-04-01T00:00:00Z2014-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.26/8076engColloids and Surfaces B: Biointerfaces. Volume 116, 1 April 2014, Pages 63–71. Doi:10.1016/j.colsurfb.2013.12.0420927-7765info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-10-06T14:51:32Zoai:comum.rcaap.pt:10400.26/8076Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:06:13.562358Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers
title Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers
spellingShingle Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers
Serro, A. P.
Tetracaine
Biomembrane models
Liposomes
Langmuir monolayers
DMPC
Cholesterol
title_short Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers
title_full Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers
title_fullStr Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers
title_full_unstemmed Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers
title_sort Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers
author Serro, A. P.
author_facet Serro, A. P.
Galante, R.
Kozica, A.
Paradiso, P.
Gonçalves da Silva, A.M.P.S.
Luzyanina, K. V.
Fernandes, A. C.
Saramago, B.
author_role author
author2 Galante, R.
Kozica, A.
Paradiso, P.
Gonçalves da Silva, A.M.P.S.
Luzyanina, K. V.
Fernandes, A. C.
Saramago, B.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Comum
dc.contributor.author.fl_str_mv Serro, A. P.
Galante, R.
Kozica, A.
Paradiso, P.
Gonçalves da Silva, A.M.P.S.
Luzyanina, K. V.
Fernandes, A. C.
Saramago, B.
dc.subject.por.fl_str_mv Tetracaine
Biomembrane models
Liposomes
Langmuir monolayers
DMPC
Cholesterol
topic Tetracaine
Biomembrane models
Liposomes
Langmuir monolayers
DMPC
Cholesterol
description “NOTICE: this is the author’s version of a work that was accepted for publication in Colloids and Surfaces B: Biointerfaces. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Colloids and Surfaces B: Biointerfaces, [Vol. 16, (April 2014)] DOI 10.1016/j.colsurfb.2013.12.042 "
publishDate 2014
dc.date.none.fl_str_mv 2014-04-01T00:00:00Z
2014-04-01T00:00:00Z
2015-03-20T09:08:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.26/8076
url http://hdl.handle.net/10400.26/8076
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Colloids and Surfaces B: Biointerfaces. Volume 116, 1 April 2014, Pages 63–71. Doi:10.1016/j.colsurfb.2013.12.042
0927-7765
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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