Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.26/8076 |
Resumo: | “NOTICE: this is the author’s version of a work that was accepted for publication in Colloids and Surfaces B: Biointerfaces. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Colloids and Surfaces B: Biointerfaces, [Vol. 16, (April 2014)] DOI 10.1016/j.colsurfb.2013.12.042 " |
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7160 |
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Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayersTetracaineBiomembrane modelsLiposomesLangmuir monolayersDMPCCholesterol“NOTICE: this is the author’s version of a work that was accepted for publication in Colloids and Surfaces B: Biointerfaces. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Colloids and Surfaces B: Biointerfaces, [Vol. 16, (April 2014)] DOI 10.1016/j.colsurfb.2013.12.042 ""Different types of lipid bilayers/monolayers have been used to simulate the cellular membranes in the investigation of the interactions between drugs and cells. However, to our knowledge, very few studies focused on the influence of the chosen membrane model upon the obtained results. The main objective of this work is to understand how do the nature and immobilization state of the biomembrane models influence the effect of the local anaesthetic tetracaine (TTC) upon the lipid membranes. The interaction of TTC with different biomembrane models of dimyristoylphosphatidylcholine (DMPC) with and without cholesterol (CHOL) was investigated through several techniques. A quartz crystal microbalance with dissipation (QCM-D) was used to study the effect on immobilized liposomes, while phosphorus nuclear magnetic resonance (31P-NMR) and differential scanning calorimetry (DSC) were applied to liposomes in suspension. The effect of TTC on Langmuir monolayers of lipids was also investigated through surface pressure-area measurements at the air-water interface. The general conclusion was that TTC has a fluidizing effect on the lipid membranes and, above certain concentrations, induced membrane swelling or even solubilization. However, different models led to variable responses to the TTC action. The intensity of the disordering effect caused by TTC increased in the following order: supported liposomes < liposomes in solution < Langmuir monolayers. This means that extrapolation of the results obtain in in vitro studies of the lipid/anaesthetic interactions to in vivo conditions should be done carefully."ElsevierRepositório ComumSerro, A. P.Galante, R.Kozica, A.Paradiso, P.Gonçalves da Silva, A.M.P.S.Luzyanina, K. V.Fernandes, A. C.Saramago, B.2015-03-20T09:08:31Z2014-04-01T00:00:00Z2014-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.26/8076engColloids and Surfaces B: Biointerfaces. Volume 116, 1 April 2014, Pages 63–71. Doi:10.1016/j.colsurfb.2013.12.0420927-7765info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-10-06T14:51:32Zoai:comum.rcaap.pt:10400.26/8076Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:06:13.562358Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers |
title |
Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers |
spellingShingle |
Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers Serro, A. P. Tetracaine Biomembrane models Liposomes Langmuir monolayers DMPC Cholesterol |
title_short |
Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers |
title_full |
Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers |
title_fullStr |
Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers |
title_full_unstemmed |
Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers |
title_sort |
Effect of tetracaine on DMPC and DMPC + cholesterol biomembrane models: Liposomes and monolayers |
author |
Serro, A. P. |
author_facet |
Serro, A. P. Galante, R. Kozica, A. Paradiso, P. Gonçalves da Silva, A.M.P.S. Luzyanina, K. V. Fernandes, A. C. Saramago, B. |
author_role |
author |
author2 |
Galante, R. Kozica, A. Paradiso, P. Gonçalves da Silva, A.M.P.S. Luzyanina, K. V. Fernandes, A. C. Saramago, B. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Comum |
dc.contributor.author.fl_str_mv |
Serro, A. P. Galante, R. Kozica, A. Paradiso, P. Gonçalves da Silva, A.M.P.S. Luzyanina, K. V. Fernandes, A. C. Saramago, B. |
dc.subject.por.fl_str_mv |
Tetracaine Biomembrane models Liposomes Langmuir monolayers DMPC Cholesterol |
topic |
Tetracaine Biomembrane models Liposomes Langmuir monolayers DMPC Cholesterol |
description |
“NOTICE: this is the author’s version of a work that was accepted for publication in Colloids and Surfaces B: Biointerfaces. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Colloids and Surfaces B: Biointerfaces, [Vol. 16, (April 2014)] DOI 10.1016/j.colsurfb.2013.12.042 " |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-01T00:00:00Z 2014-04-01T00:00:00Z 2015-03-20T09:08:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.26/8076 |
url |
http://hdl.handle.net/10400.26/8076 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Colloids and Surfaces B: Biointerfaces. Volume 116, 1 April 2014, Pages 63–71. Doi:10.1016/j.colsurfb.2013.12.042 0927-7765 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799129933456343040 |