Bioactive properties and phenolic profile of Momordica charantia L. medicinal plant growing wild in Trinidad and Tobago
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/15266 |
Resumo: | A wild variety of bitter melon Momordica charantia L. (Cucurbitaceae) has been used in bush medicine of Trinidad and Tobago for treatment of diabetes, inflammations and cancer. Despite many studies regarding the cultivated bitter melon, the wild variety has been poorly investigated. This study evaluates the biological activities of the ethanol/water extract of aerial parts and correlates these activities with the presence of phenolic compounds. The extract exhibited antioxidant activity in the four assays (DPPH, reducing power, ß-carotene bleaching and TBARS). The key role of oxidative stress in inflammation and tumorigenesis was supported by the results of anti-inflammatory (inhibition of nitric oxide production) and cytotoxicity (human tumor cell lines, namely HeLa, HepG2, MCF-7, and NCI-H460) assays. In contrast, no toxicity was observed in non-tumor cells. In the antibacterial screening, clinical resistant isolates were significantly affected (MIC 50 = 10–0.625 µg/mL), being Listeria monocytogenes the most susceptible. Three phenolic acids and eleven flavonol glycosides derivatives were identified, quercetin-3-O-pentosylhexoside being the most abundant. |
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Bioactive properties and phenolic profile of Momordica charantia L. medicinal plant growing wild in Trinidad and TobagoAnti-inflammatoryAntimicrobial activityAntioxidantCytotoxicityMomordica charantia L.Phenolic compoundsA wild variety of bitter melon Momordica charantia L. (Cucurbitaceae) has been used in bush medicine of Trinidad and Tobago for treatment of diabetes, inflammations and cancer. Despite many studies regarding the cultivated bitter melon, the wild variety has been poorly investigated. This study evaluates the biological activities of the ethanol/water extract of aerial parts and correlates these activities with the presence of phenolic compounds. The extract exhibited antioxidant activity in the four assays (DPPH, reducing power, ß-carotene bleaching and TBARS). The key role of oxidative stress in inflammation and tumorigenesis was supported by the results of anti-inflammatory (inhibition of nitric oxide production) and cytotoxicity (human tumor cell lines, namely HeLa, HepG2, MCF-7, and NCI-H460) assays. In contrast, no toxicity was observed in non-tumor cells. In the antibacterial screening, clinical resistant isolates were significantly affected (MIC 50 = 10–0.625 µg/mL), being Listeria monocytogenes the most susceptible. Three phenolic acids and eleven flavonol glycosides derivatives were identified, quercetin-3-O-pentosylhexoside being the most abundant.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2013), LSRE (Project UID/EQU/50020/2013), L. Barros (SFRH/BPD/107855/2015), S. Heleno (SFRH/BPD/101413/2014) and R.C. Calhelha (SFRH/BPD/BPD/68344/2010) grants. This study was financially supported by Internal Grant Agency of Tomas Bata University in Zlin, project Nr. IGA/FT/2016/003.Biblioteca Digital do IPBSvobodova, BlankaBarros, LillianCalhelha, Ricardo C.Heleno, Sandrina A.Alves, Maria JoséWalcott, SimoneBittova, MiroslavaKuban, VlastimilFerreira, Isabel C.F.R.2018-01-25T10:00:00Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/15266engSvobodova, Blanka; Barros, Lillian; Calhelha, Ricardo C.; Heleno, Sandrina; Alves, Maria Jose; Walcott, Simone; Bittova, Miroslava; Kuban, Vlastimil; Ferreira, Isabel C.F.R. (2017). Bioactive properties and phenolic profile of Momordica charantia L. medicinal plant growing wild in Trinidad and Tobago. Industrial Crops and Products. ISSN 0926-6690. 95, p. 365-3730926-669010.1016/j.indcrop.2016.10.046info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:35:42Zoai:bibliotecadigital.ipb.pt:10198/15266Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:04:53.946297Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bioactive properties and phenolic profile of Momordica charantia L. medicinal plant growing wild in Trinidad and Tobago |
title |
Bioactive properties and phenolic profile of Momordica charantia L. medicinal plant growing wild in Trinidad and Tobago |
spellingShingle |
Bioactive properties and phenolic profile of Momordica charantia L. medicinal plant growing wild in Trinidad and Tobago Svobodova, Blanka Anti-inflammatory Antimicrobial activity Antioxidant Cytotoxicity Momordica charantia L. Phenolic compounds |
title_short |
Bioactive properties and phenolic profile of Momordica charantia L. medicinal plant growing wild in Trinidad and Tobago |
title_full |
Bioactive properties and phenolic profile of Momordica charantia L. medicinal plant growing wild in Trinidad and Tobago |
title_fullStr |
Bioactive properties and phenolic profile of Momordica charantia L. medicinal plant growing wild in Trinidad and Tobago |
title_full_unstemmed |
Bioactive properties and phenolic profile of Momordica charantia L. medicinal plant growing wild in Trinidad and Tobago |
title_sort |
Bioactive properties and phenolic profile of Momordica charantia L. medicinal plant growing wild in Trinidad and Tobago |
author |
Svobodova, Blanka |
author_facet |
Svobodova, Blanka Barros, Lillian Calhelha, Ricardo C. Heleno, Sandrina A. Alves, Maria José Walcott, Simone Bittova, Miroslava Kuban, Vlastimil Ferreira, Isabel C.F.R. |
author_role |
author |
author2 |
Barros, Lillian Calhelha, Ricardo C. Heleno, Sandrina A. Alves, Maria José Walcott, Simone Bittova, Miroslava Kuban, Vlastimil Ferreira, Isabel C.F.R. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Svobodova, Blanka Barros, Lillian Calhelha, Ricardo C. Heleno, Sandrina A. Alves, Maria José Walcott, Simone Bittova, Miroslava Kuban, Vlastimil Ferreira, Isabel C.F.R. |
dc.subject.por.fl_str_mv |
Anti-inflammatory Antimicrobial activity Antioxidant Cytotoxicity Momordica charantia L. Phenolic compounds |
topic |
Anti-inflammatory Antimicrobial activity Antioxidant Cytotoxicity Momordica charantia L. Phenolic compounds |
description |
A wild variety of bitter melon Momordica charantia L. (Cucurbitaceae) has been used in bush medicine of Trinidad and Tobago for treatment of diabetes, inflammations and cancer. Despite many studies regarding the cultivated bitter melon, the wild variety has been poorly investigated. This study evaluates the biological activities of the ethanol/water extract of aerial parts and correlates these activities with the presence of phenolic compounds. The extract exhibited antioxidant activity in the four assays (DPPH, reducing power, ß-carotene bleaching and TBARS). The key role of oxidative stress in inflammation and tumorigenesis was supported by the results of anti-inflammatory (inhibition of nitric oxide production) and cytotoxicity (human tumor cell lines, namely HeLa, HepG2, MCF-7, and NCI-H460) assays. In contrast, no toxicity was observed in non-tumor cells. In the antibacterial screening, clinical resistant isolates were significantly affected (MIC 50 = 10–0.625 µg/mL), being Listeria monocytogenes the most susceptible. Three phenolic acids and eleven flavonol glycosides derivatives were identified, quercetin-3-O-pentosylhexoside being the most abundant. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z 2018-01-25T10:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/15266 |
url |
http://hdl.handle.net/10198/15266 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Svobodova, Blanka; Barros, Lillian; Calhelha, Ricardo C.; Heleno, Sandrina; Alves, Maria Jose; Walcott, Simone; Bittova, Miroslava; Kuban, Vlastimil; Ferreira, Isabel C.F.R. (2017). Bioactive properties and phenolic profile of Momordica charantia L. medicinal plant growing wild in Trinidad and Tobago. Industrial Crops and Products. ISSN 0926-6690. 95, p. 365-373 0926-6690 10.1016/j.indcrop.2016.10.046 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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