Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst

Detalhes bibliográficos
Autor(a) principal: Escórcio, Rita
Data de Publicação: 2023
Outros Autores: Sandhu, Armaan K., Bento, Artur, Tomé, Ana S., Moreira, Carlos J.S., Brözel, Volker S., Silva Pereira, Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158529
Resumo: Funding Information: The authors acknowledge CERMAX, ITQB-NOVA, Oeiras, Portugal where the NMR data were acquired with equipment funded by FCT. The authors are thankful to Giovanna Martins for technical support during her BSc internship. Funding Information: Funding from the European Research Council through grant ERC CoG-647928, from the Office of Naval Research Global (ONRG, RESEARCH GRANT—N62909-23-1-2007), and from Fundação para a Ciência e Tecnologia (FCT) by Project MOSTMICRO ITQB with refs UIDB/04612/2020 and UIDP/04612/2020 and LS4FUTURE Associated Laboratory (LA/P/0087/2020). RE is grateful to FCT funding for her PhD scholarship (2021.06435.BD). Publisher Copyright: Copyright © 2023 Escórcio, Sandhu, Bento, Tomé, Moreira, Brözel and Silva Pereira.
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spelling Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalystGlycine maxpurification of a suberin polymerquantification of polymeric featuressoybean rootsuberin in plantaChemistry(all)Funding Information: The authors acknowledge CERMAX, ITQB-NOVA, Oeiras, Portugal where the NMR data were acquired with equipment funded by FCT. The authors are thankful to Giovanna Martins for technical support during her BSc internship. Funding Information: Funding from the European Research Council through grant ERC CoG-647928, from the Office of Naval Research Global (ONRG, RESEARCH GRANT—N62909-23-1-2007), and from Fundação para a Ciência e Tecnologia (FCT) by Project MOSTMICRO ITQB with refs UIDB/04612/2020 and UIDP/04612/2020 and LS4FUTURE Associated Laboratory (LA/P/0087/2020). RE is grateful to FCT funding for her PhD scholarship (2021.06435.BD). Publisher Copyright: Copyright © 2023 Escórcio, Sandhu, Bento, Tomé, Moreira, Brözel and Silva Pereira.Soybean (Glycine max) is an increasingly relevant crop due to its economic importance and also a model plant for the study of root symbiotic associations with nodule forming rhizobia. Plant polyesters mediate plant-microbe interactions with both pathogenic and beneficial microbes; suberin has been hypothesized to play a key role during the early steps of rhizobia attachment to the root. The downside is that suberin chemistry in soybean root is still scarcely studied. This study addresses this outstanding question by reporting a straightforward workflow for a speedy purification of suberin from soybean root and for its subsequent detailed chemical analysis. To purify suberin, cholinium hexanoate (an ionic liquid) was used as the catalyst. The ensuing suberin is highly esterified as observed by a precise Nuclear Magnetic Resonance quantification of each ester type, discriminating between primary and acylglycerol esters. Moreover, the composing hydrolysable monomers detected through GC-MS revealed that hexadecanoic acid is the most abundant monomer, similar to that reported before by others. Overall, this study highlights the adequacy of the ionic liquid catalyst for the isolation of suberin from soybean roots, where the polymer natural abundance is low, and builds new knowledge on the specificities of its chemistry; essential to better understand the biological roles of suberin in roots.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNEscórcio, RitaSandhu, Armaan K.Bento, ArturTomé, Ana S.Moreira, Carlos J.S.Brözel, Volker S.Silva Pereira, Cristina2023-09-30T22:19:05Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158529eng2296-2646PURE: 72375230https://doi.org/10.3389/fchem.2023.1165234info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:14:50Zoai:run.unl.pt:10362/158529Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:14:50Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst
title Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst
spellingShingle Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst
Escórcio, Rita
Glycine max
purification of a suberin polymer
quantification of polymeric features
soybean root
suberin in planta
Chemistry(all)
title_short Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst
title_full Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst
title_fullStr Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst
title_full_unstemmed Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst
title_sort Purification of archetypal soybean root suberin mostly comprising alka(e)noic acids using an ionic liquid catalyst
author Escórcio, Rita
author_facet Escórcio, Rita
Sandhu, Armaan K.
Bento, Artur
Tomé, Ana S.
Moreira, Carlos J.S.
Brözel, Volker S.
Silva Pereira, Cristina
author_role author
author2 Sandhu, Armaan K.
Bento, Artur
Tomé, Ana S.
Moreira, Carlos J.S.
Brözel, Volker S.
Silva Pereira, Cristina
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Escórcio, Rita
Sandhu, Armaan K.
Bento, Artur
Tomé, Ana S.
Moreira, Carlos J.S.
Brözel, Volker S.
Silva Pereira, Cristina
dc.subject.por.fl_str_mv Glycine max
purification of a suberin polymer
quantification of polymeric features
soybean root
suberin in planta
Chemistry(all)
topic Glycine max
purification of a suberin polymer
quantification of polymeric features
soybean root
suberin in planta
Chemistry(all)
description Funding Information: The authors acknowledge CERMAX, ITQB-NOVA, Oeiras, Portugal where the NMR data were acquired with equipment funded by FCT. The authors are thankful to Giovanna Martins for technical support during her BSc internship. Funding Information: Funding from the European Research Council through grant ERC CoG-647928, from the Office of Naval Research Global (ONRG, RESEARCH GRANT—N62909-23-1-2007), and from Fundação para a Ciência e Tecnologia (FCT) by Project MOSTMICRO ITQB with refs UIDB/04612/2020 and UIDP/04612/2020 and LS4FUTURE Associated Laboratory (LA/P/0087/2020). RE is grateful to FCT funding for her PhD scholarship (2021.06435.BD). Publisher Copyright: Copyright © 2023 Escórcio, Sandhu, Bento, Tomé, Moreira, Brözel and Silva Pereira.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-30T22:19:05Z
2023
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158529
url http://hdl.handle.net/10362/158529
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2296-2646
PURE: 72375230
https://doi.org/10.3389/fchem.2023.1165234
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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