Thermodynamic and functional characterization of the periplasmic triheme cytochrome PpcA from Geobacter metallireducens

Detalhes bibliográficos
Autor(a) principal: Fernandes, Tomás M.
Data de Publicação: 2018
Outros Autores: Morgado, Leonor, Salgueiro, Carlos A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1042/BCJ20180457
Resumo: SFRH/BPD/114848/2016. PTDC/BBB-BQB/3554/2014. POCI-01-0145-FEDER-007728 National NMR Network (PTNMR) and are supported by Infrastructure Project No022161.
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spelling Thermodynamic and functional characterization of the periplasmic triheme cytochrome PpcA from Geobacter metallireducensBiochemistryMolecular BiologyCell BiologySFRH/BPD/114848/2016. PTDC/BBB-BQB/3554/2014. POCI-01-0145-FEDER-007728 National NMR Network (PTNMR) and are supported by Infrastructure Project No022161.The Geobacter metallireducens bacterium can couple the oxidation of a wide range of compounds to the reduction of several extracellular electron acceptors, including pollutants or electrode surfaces for current production in microbial fuel cells. For these reasons, G. metallireducens are of interest for practical biotechnological applications. The use of such electron acceptors relies on a mechanism that permits electrons to be transferred to the cell exterior. The cytochrome PpcA from G. metallireducens is a member of a family composed of five periplasmic triheme cytochromes, which are important to bridge the electron transfer from the cytoplasmic donors to the extracellular acceptors. Using NMR and visible spectroscopic techniques, a detailed thermodynamic characterization of PpcA was obtained, including the determination of the heme reduction potentials and their redox and redox-Bohr interactions. These parameters revealed unique features for PpcA from G. metallireducens compared with other triheme cytochromes from different microorganisms, namely the less negative heme reduction potentials and concomitant functional working potential ranges. It was also shown that the order of oxidation of the hemes is pH-independent, but the protein is designed to couple e − /H + transfer exclusively at physiological pH.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNFernandes, Tomás M.Morgado, LeonorSalgueiro, Carlos A.2022-02-16T01:31:08Z2018-09-142018-09-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttps://doi.org/10.1042/BCJ20180457eng0264-6021PURE: 13710556http://www.scopus.com/inward/record.url?scp=85061197673&partnerID=8YFLogxKhttps://doi.org/10.1042/BCJ20180457info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:34:26Zoai:run.unl.pt:10362/75192Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:35:29.601834Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Thermodynamic and functional characterization of the periplasmic triheme cytochrome PpcA from Geobacter metallireducens
title Thermodynamic and functional characterization of the periplasmic triheme cytochrome PpcA from Geobacter metallireducens
spellingShingle Thermodynamic and functional characterization of the periplasmic triheme cytochrome PpcA from Geobacter metallireducens
Fernandes, Tomás M.
Biochemistry
Molecular Biology
Cell Biology
title_short Thermodynamic and functional characterization of the periplasmic triheme cytochrome PpcA from Geobacter metallireducens
title_full Thermodynamic and functional characterization of the periplasmic triheme cytochrome PpcA from Geobacter metallireducens
title_fullStr Thermodynamic and functional characterization of the periplasmic triheme cytochrome PpcA from Geobacter metallireducens
title_full_unstemmed Thermodynamic and functional characterization of the periplasmic triheme cytochrome PpcA from Geobacter metallireducens
title_sort Thermodynamic and functional characterization of the periplasmic triheme cytochrome PpcA from Geobacter metallireducens
author Fernandes, Tomás M.
author_facet Fernandes, Tomás M.
Morgado, Leonor
Salgueiro, Carlos A.
author_role author
author2 Morgado, Leonor
Salgueiro, Carlos A.
author2_role author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Fernandes, Tomás M.
Morgado, Leonor
Salgueiro, Carlos A.
dc.subject.por.fl_str_mv Biochemistry
Molecular Biology
Cell Biology
topic Biochemistry
Molecular Biology
Cell Biology
description SFRH/BPD/114848/2016. PTDC/BBB-BQB/3554/2014. POCI-01-0145-FEDER-007728 National NMR Network (PTNMR) and are supported by Infrastructure Project No022161.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-14
2018-09-14T00:00:00Z
2022-02-16T01:31:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1042/BCJ20180457
url https://doi.org/10.1042/BCJ20180457
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0264-6021
PURE: 13710556
http://www.scopus.com/inward/record.url?scp=85061197673&partnerID=8YFLogxK
https://doi.org/10.1042/BCJ20180457
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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instacron:RCAAP
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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