Characterization of a new podovirus infecting Paenibacillus larvae
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/64317 |
Resumo: | The Paenibacillus larvae infecting phage API480 (vB_PlaP_API480) is the first reported podovirus for this bacterial species, with an 58nm icosahedral capsid and a 12×8nm short, non-contractile tail. API480 encodes 77 coding sequences (CDSs) on its 45,026bp dsDNA genome, of which 47 were confirmed using mass spectrometry. This phage has got very limited genomic and proteomic similarity to any other known ones registered in public databases, including P. larvae phages. Comparative genomics indicates API480 is a new species as its a singleton with 28 unique proteins. Interestingly, the lysis module is highly conserved among P. larvae phages, containing a predicted endolysin and two putative holins. The well kept overall genomic organisation (from the structural and morphogenetic modules to the host lysis, DNA replication and metabolism related proteins) confirms a common evolutionary ancestor among P. larvae infecting phages. API480 is able to infect 69% of the 61 field strains with an ERIC I genotype, as well as ERIC II strains. Furthermore, this phage is very stable when exposed to high glucose concentrations and to larval gastrointestinal conditions. This highly-specific phage, with its broad lytic activity and stability in hive conditions, might potentially be used in the biocontrol of American Foulbrood (AFB). |
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Characterization of a new podovirus infecting Paenibacillus larvaeScience & TechnologyThe Paenibacillus larvae infecting phage API480 (vB_PlaP_API480) is the first reported podovirus for this bacterial species, with an 58nm icosahedral capsid and a 12×8nm short, non-contractile tail. API480 encodes 77 coding sequences (CDSs) on its 45,026bp dsDNA genome, of which 47 were confirmed using mass spectrometry. This phage has got very limited genomic and proteomic similarity to any other known ones registered in public databases, including P. larvae phages. Comparative genomics indicates API480 is a new species as its a singleton with 28 unique proteins. Interestingly, the lysis module is highly conserved among P. larvae phages, containing a predicted endolysin and two putative holins. The well kept overall genomic organisation (from the structural and morphogenetic modules to the host lysis, DNA replication and metabolism related proteins) confirms a common evolutionary ancestor among P. larvae infecting phages. API480 is able to infect 69% of the 61 field strains with an ERIC I genotype, as well as ERIC II strains. Furthermore, this phage is very stable when exposed to high glucose concentrations and to larval gastrointestinal conditions. This highly-specific phage, with its broad lytic activity and stability in hive conditions, might potentially be used in the biocontrol of American Foulbrood (AFB).Te authors would like to aknowledge Dr. Mariano Higes Pascual from Laboratorio de Patología Apícola del Centro de Investigación Apícola y Agroambiental de Marchamalo (CIAPA-IRIAF) for gently providing us P. larvae isolates and the soil sample from which API480 was isolated. Tis study, carried out under the scope of the project APILYSE, PTDC/CVT-EPI/4008/2014 - POCI-01–0145-FEDER-016598, was supported by FCT - Fundação para a Ciência e a Tecnologia, under the scope of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020- Programa Operacional Regional do Norte. HR was supported by FCT through the grant SFRH/BD/128859/2017. MB and RL were supported by KU Leuven through a GOA grant [3E140356].info:eu-repo/semantics/publishedVersionNature ResearchUniversidade do MinhoRibeiro, Henrique G.Melo, Luís Daniel RodriguesOliveira, Hugo Alexandre MendesBoon, MaartenLavigne, RobNoben, Jean-PaulAzeredo, JoanaOliveira, A.2019-12-302019-12-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/64317engRibeiro, Henrique G.; Melo, Luís D. R.; Oliveira, Hugo; Boon, Maarten; Lavigne, Rob; Noben, Jean-Paul; Azeredo, Joana; Oliveira, A., Characterization of a new podovirus infecting Paenibacillus larvae. Scientific Reports, 9(20355), 20192045-23222045-232210.1038/s41598-019-56699-y31889094http://www.nature.com/srep/index.htmlinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:43:58Zoai:repositorium.sdum.uminho.pt:1822/64317Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:41:35.126811Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Characterization of a new podovirus infecting Paenibacillus larvae |
title |
Characterization of a new podovirus infecting Paenibacillus larvae |
spellingShingle |
Characterization of a new podovirus infecting Paenibacillus larvae Ribeiro, Henrique G. Science & Technology |
title_short |
Characterization of a new podovirus infecting Paenibacillus larvae |
title_full |
Characterization of a new podovirus infecting Paenibacillus larvae |
title_fullStr |
Characterization of a new podovirus infecting Paenibacillus larvae |
title_full_unstemmed |
Characterization of a new podovirus infecting Paenibacillus larvae |
title_sort |
Characterization of a new podovirus infecting Paenibacillus larvae |
author |
Ribeiro, Henrique G. |
author_facet |
Ribeiro, Henrique G. Melo, Luís Daniel Rodrigues Oliveira, Hugo Alexandre Mendes Boon, Maarten Lavigne, Rob Noben, Jean-Paul Azeredo, Joana Oliveira, A. |
author_role |
author |
author2 |
Melo, Luís Daniel Rodrigues Oliveira, Hugo Alexandre Mendes Boon, Maarten Lavigne, Rob Noben, Jean-Paul Azeredo, Joana Oliveira, A. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Ribeiro, Henrique G. Melo, Luís Daniel Rodrigues Oliveira, Hugo Alexandre Mendes Boon, Maarten Lavigne, Rob Noben, Jean-Paul Azeredo, Joana Oliveira, A. |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
The Paenibacillus larvae infecting phage API480 (vB_PlaP_API480) is the first reported podovirus for this bacterial species, with an 58nm icosahedral capsid and a 12×8nm short, non-contractile tail. API480 encodes 77 coding sequences (CDSs) on its 45,026bp dsDNA genome, of which 47 were confirmed using mass spectrometry. This phage has got very limited genomic and proteomic similarity to any other known ones registered in public databases, including P. larvae phages. Comparative genomics indicates API480 is a new species as its a singleton with 28 unique proteins. Interestingly, the lysis module is highly conserved among P. larvae phages, containing a predicted endolysin and two putative holins. The well kept overall genomic organisation (from the structural and morphogenetic modules to the host lysis, DNA replication and metabolism related proteins) confirms a common evolutionary ancestor among P. larvae infecting phages. API480 is able to infect 69% of the 61 field strains with an ERIC I genotype, as well as ERIC II strains. Furthermore, this phage is very stable when exposed to high glucose concentrations and to larval gastrointestinal conditions. This highly-specific phage, with its broad lytic activity and stability in hive conditions, might potentially be used in the biocontrol of American Foulbrood (AFB). |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-30 2019-12-30T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/64317 |
url |
http://hdl.handle.net/1822/64317 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ribeiro, Henrique G.; Melo, Luís D. R.; Oliveira, Hugo; Boon, Maarten; Lavigne, Rob; Noben, Jean-Paul; Azeredo, Joana; Oliveira, A., Characterization of a new podovirus infecting Paenibacillus larvae. Scientific Reports, 9(20355), 2019 2045-2322 2045-2322 10.1038/s41598-019-56699-y 31889094 http://www.nature.com/srep/index.html |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Research |
publisher.none.fl_str_mv |
Nature Research |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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