Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus

Detalhes bibliográficos
Autor(a) principal: Ongenae, Veronique
Data de Publicação: 2022
Outros Autores: Azeredo, Joana, Kropinski, Andrew M., Rozen, Daniel, Briegel, Ariane, Claessen, Dennis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/80409
Resumo: Streptomycetes are ubiquitous soil bacteria. Here we report the complete and annotated genome sequence and characterization of Streptomyces phage Pablito, isolated from a soil sample in Haarlem, the Netherlands using Streptomyces lividans as host. This phage was able to infect a diverse range of Streptomyces strains, but none belonging to the sister genus Kitasatospora. Phage Pablito has double-stranded DNA with a genome length of 49,581 base pairs encoding 76 putative proteins, of which 26 could be predicted. The presence of a serine integrase protein indicated the lysogenic nature of phage Pablito. The phage remained stable over a wide range of temperatures (25--45 \textdegreeC) and at pH\thinspace\thinspace7.0, but lost infectivity at temperatures above 55 \textdegreeC or when the pH dropped below 6.0. This newly isolated phage is closely related to Streptomyces phage Janus and Hank144 and considered a new species classified in the genus Janusvirus, within the subfamily Arquattrovirinae.
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spelling Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus JanusvirusScience & TechnologyStreptomycetes are ubiquitous soil bacteria. Here we report the complete and annotated genome sequence and characterization of Streptomyces phage Pablito, isolated from a soil sample in Haarlem, the Netherlands using Streptomyces lividans as host. This phage was able to infect a diverse range of Streptomyces strains, but none belonging to the sister genus Kitasatospora. Phage Pablito has double-stranded DNA with a genome length of 49,581 base pairs encoding 76 putative proteins, of which 26 could be predicted. The presence of a serine integrase protein indicated the lysogenic nature of phage Pablito. The phage remained stable over a wide range of temperatures (25--45 \textdegreeC) and at pH\thinspace\thinspace7.0, but lost infectivity at temperatures above 55 \textdegreeC or when the pH dropped below 6.0. This newly isolated phage is closely related to Streptomyces phage Janus and Hank144 and considered a new species classified in the genus Janusvirus, within the subfamily Arquattrovirinae.This work was funded by a VICI grant from the Dutch Research Council (NWO) to DC (Grant Number VI.C.192.002) and an NWO XS grant to AB (Grant Number OCENW.XS.041). Microscope access was supported by the Netherlands Center for Electron Nanoscopy and partially funded by the Netherlands Electron Microscopy Infrastructure Grant 84.034.014.info:eu-repo/semantics/publishedVersionNature ResearchUniversidade do MinhoOngenae, VeroniqueAzeredo, JoanaKropinski, Andrew M.Rozen, DanielBriegel, ArianeClaessen, Dennis2022-10-222022-10-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/80409engOngenae, V., Azeredo, J., Kropinski, A.M. et al. Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus. Sci Rep 12, 17785 (2022). https://doi.org/10.1038/s41598-022-22784-y2045232210.1038/s41598-022-22784-y36273096https://www.nature.com/articles/s41598-022-22784-yinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:09:30Zoai:repositorium.sdum.uminho.pt:1822/80409Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:00:54.881334Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus
title Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus
spellingShingle Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus
Ongenae, Veronique
Science & Technology
title_short Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus
title_full Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus
title_fullStr Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus
title_full_unstemmed Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus
title_sort Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus
author Ongenae, Veronique
author_facet Ongenae, Veronique
Azeredo, Joana
Kropinski, Andrew M.
Rozen, Daniel
Briegel, Ariane
Claessen, Dennis
author_role author
author2 Azeredo, Joana
Kropinski, Andrew M.
Rozen, Daniel
Briegel, Ariane
Claessen, Dennis
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Ongenae, Veronique
Azeredo, Joana
Kropinski, Andrew M.
Rozen, Daniel
Briegel, Ariane
Claessen, Dennis
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description Streptomycetes are ubiquitous soil bacteria. Here we report the complete and annotated genome sequence and characterization of Streptomyces phage Pablito, isolated from a soil sample in Haarlem, the Netherlands using Streptomyces lividans as host. This phage was able to infect a diverse range of Streptomyces strains, but none belonging to the sister genus Kitasatospora. Phage Pablito has double-stranded DNA with a genome length of 49,581 base pairs encoding 76 putative proteins, of which 26 could be predicted. The presence of a serine integrase protein indicated the lysogenic nature of phage Pablito. The phage remained stable over a wide range of temperatures (25--45 \textdegreeC) and at pH\thinspace\thinspace7.0, but lost infectivity at temperatures above 55 \textdegreeC or when the pH dropped below 6.0. This newly isolated phage is closely related to Streptomyces phage Janus and Hank144 and considered a new species classified in the genus Janusvirus, within the subfamily Arquattrovirinae.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-22
2022-10-22T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/80409
url https://hdl.handle.net/1822/80409
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ongenae, V., Azeredo, J., Kropinski, A.M. et al. Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus. Sci Rep 12, 17785 (2022). https://doi.org/10.1038/s41598-022-22784-y
20452322
10.1038/s41598-022-22784-y
36273096
https://www.nature.com/articles/s41598-022-22784-y
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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