Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/80409 |
Resumo: | Streptomycetes are ubiquitous soil bacteria. Here we report the complete and annotated genome sequence and characterization of Streptomyces phage Pablito, isolated from a soil sample in Haarlem, the Netherlands using Streptomyces lividans as host. This phage was able to infect a diverse range of Streptomyces strains, but none belonging to the sister genus Kitasatospora. Phage Pablito has double-stranded DNA with a genome length of 49,581 base pairs encoding 76 putative proteins, of which 26 could be predicted. The presence of a serine integrase protein indicated the lysogenic nature of phage Pablito. The phage remained stable over a wide range of temperatures (25--45 \textdegreeC) and at pH\thinspace\thinspace7.0, but lost infectivity at temperatures above 55 \textdegreeC or when the pH dropped below 6.0. This newly isolated phage is closely related to Streptomyces phage Janus and Hank144 and considered a new species classified in the genus Janusvirus, within the subfamily Arquattrovirinae. |
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Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus JanusvirusScience & TechnologyStreptomycetes are ubiquitous soil bacteria. Here we report the complete and annotated genome sequence and characterization of Streptomyces phage Pablito, isolated from a soil sample in Haarlem, the Netherlands using Streptomyces lividans as host. This phage was able to infect a diverse range of Streptomyces strains, but none belonging to the sister genus Kitasatospora. Phage Pablito has double-stranded DNA with a genome length of 49,581 base pairs encoding 76 putative proteins, of which 26 could be predicted. The presence of a serine integrase protein indicated the lysogenic nature of phage Pablito. The phage remained stable over a wide range of temperatures (25--45 \textdegreeC) and at pH\thinspace\thinspace7.0, but lost infectivity at temperatures above 55 \textdegreeC or when the pH dropped below 6.0. This newly isolated phage is closely related to Streptomyces phage Janus and Hank144 and considered a new species classified in the genus Janusvirus, within the subfamily Arquattrovirinae.This work was funded by a VICI grant from the Dutch Research Council (NWO) to DC (Grant Number VI.C.192.002) and an NWO XS grant to AB (Grant Number OCENW.XS.041). Microscope access was supported by the Netherlands Center for Electron Nanoscopy and partially funded by the Netherlands Electron Microscopy Infrastructure Grant 84.034.014.info:eu-repo/semantics/publishedVersionNature ResearchUniversidade do MinhoOngenae, VeroniqueAzeredo, JoanaKropinski, Andrew M.Rozen, DanielBriegel, ArianeClaessen, Dennis2022-10-222022-10-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/80409engOngenae, V., Azeredo, J., Kropinski, A.M. et al. Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus. Sci Rep 12, 17785 (2022). https://doi.org/10.1038/s41598-022-22784-y2045232210.1038/s41598-022-22784-y36273096https://www.nature.com/articles/s41598-022-22784-yinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:09:30Zoai:repositorium.sdum.uminho.pt:1822/80409Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:00:54.881334Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus |
title |
Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus |
spellingShingle |
Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus Ongenae, Veronique Science & Technology |
title_short |
Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus |
title_full |
Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus |
title_fullStr |
Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus |
title_full_unstemmed |
Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus |
title_sort |
Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus |
author |
Ongenae, Veronique |
author_facet |
Ongenae, Veronique Azeredo, Joana Kropinski, Andrew M. Rozen, Daniel Briegel, Ariane Claessen, Dennis |
author_role |
author |
author2 |
Azeredo, Joana Kropinski, Andrew M. Rozen, Daniel Briegel, Ariane Claessen, Dennis |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Ongenae, Veronique Azeredo, Joana Kropinski, Andrew M. Rozen, Daniel Briegel, Ariane Claessen, Dennis |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
Streptomycetes are ubiquitous soil bacteria. Here we report the complete and annotated genome sequence and characterization of Streptomyces phage Pablito, isolated from a soil sample in Haarlem, the Netherlands using Streptomyces lividans as host. This phage was able to infect a diverse range of Streptomyces strains, but none belonging to the sister genus Kitasatospora. Phage Pablito has double-stranded DNA with a genome length of 49,581 base pairs encoding 76 putative proteins, of which 26 could be predicted. The presence of a serine integrase protein indicated the lysogenic nature of phage Pablito. The phage remained stable over a wide range of temperatures (25--45 \textdegreeC) and at pH\thinspace\thinspace7.0, but lost infectivity at temperatures above 55 \textdegreeC or when the pH dropped below 6.0. This newly isolated phage is closely related to Streptomyces phage Janus and Hank144 and considered a new species classified in the genus Janusvirus, within the subfamily Arquattrovirinae. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-22 2022-10-22T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/80409 |
url |
https://hdl.handle.net/1822/80409 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ongenae, V., Azeredo, J., Kropinski, A.M. et al. Genome sequence and characterization of Streptomyces phage Pablito, representing a new species within the genus Janusvirus. Sci Rep 12, 17785 (2022). https://doi.org/10.1038/s41598-022-22784-y 20452322 10.1038/s41598-022-22784-y 36273096 https://www.nature.com/articles/s41598-022-22784-y |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Nature Research |
publisher.none.fl_str_mv |
Nature Research |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132405617917952 |