The isoepoxydon dehydrogenase gene PCR profile is useful in fungal taxonomy
Autor(a) principal: | |
---|---|
Data de Publicação: | 2007 |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/7738 |
Resumo: | This study evaluates the specificity of PCR isoepoxydon dehydrogenase (idh) primers on fungi associated with patulin production. The DNAs of 93 strains were extracted and analysed by PCR using primers of the idh gene of patulin biosynthesis. A single band at 620 bp was obtained on 17% of the analysed strains. Different molecular weight amplicons were observed in other strains. These were employed as binary characters for numerical analysis to obtain a dendrogram. Clusters were observed, which corresponded to morphological identifications in some cases. Amplicons at 400 and/or 500 bp were related to patulin non-detection for strains, whereas a 450 bp amplicon was associated with some Aspergillus and both of the Byssochlamys nivea strains tested. Hence, the idh primers are not specific for the gene and provide other amplicon products in other species. These results were useful providing (a) profiles of DNA to identify and classify fungi and (b) insights into patulin production. The DNA profiles in this study may be useful for determining patulin producing fungi. Obtaining multiple bands in culture-independent PCR of environmental samples by using the primers could indicate that more than one species is present. |
id |
RCAP_92697adef876bd928e0a067b6b7fdd33 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/7738 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
The isoepoxydon dehydrogenase gene PCR profile is useful in fungal taxonomyPatulinPCRNumerical analysisPenicilliumAspergillusByssochlamysGen de la isoepoxydon deshidrogenasaIsoepoxydon dehydrogenase genePatulinaAnálisis numéricoThis study evaluates the specificity of PCR isoepoxydon dehydrogenase (idh) primers on fungi associated with patulin production. The DNAs of 93 strains were extracted and analysed by PCR using primers of the idh gene of patulin biosynthesis. A single band at 620 bp was obtained on 17% of the analysed strains. Different molecular weight amplicons were observed in other strains. These were employed as binary characters for numerical analysis to obtain a dendrogram. Clusters were observed, which corresponded to morphological identifications in some cases. Amplicons at 400 and/or 500 bp were related to patulin non-detection for strains, whereas a 450 bp amplicon was associated with some Aspergillus and both of the Byssochlamys nivea strains tested. Hence, the idh primers are not specific for the gene and provide other amplicon products in other species. These results were useful providing (a) profiles of DNA to identify and classify fungi and (b) insights into patulin production. The DNA profiles in this study may be useful for determining patulin producing fungi. Obtaining multiple bands in culture-independent PCR of environmental samples by using the primers could indicate that more than one species is present.El interés del presente estudio fue investigar la especificidad de los cebadores para la PCR del gen de la isoepoxydon deshidrogenasa en hongos asociados a la producción de patulina. El DNA de 93 cepas fue extraído y analizado mediante PCR utilizando cebadores del gen idh implicado en la biosíntesis de la patulina. Se obtuvo una banda simple de 620 pb en un 17% de las cepas y bandas de peso molecular variable para el resto. Estos datos fueron utilizados como caracteres binarios en un análisis numérico. Se pudo comprobar que los diferentes clusters que aparecían en el correspondiente dendograma a veces se correspondían con determinados caracteres morfológicos de las cepas. Amplificados de de 400 y/o 500 pb se relacionaron con cepas no productoras de patulina y una banda de 450 pb se asoció con algunos Aspergillus y con las dos cepas de Byssochlamys nivea incluidas en el estudio. Ello demostraba que los cebadores no eran específicos para el gen y amplificaban otras regiones en algunas especies. Estos resultados demostraron ser útiles en para la identificación y clasificación de hongos y para un mejor conocimiento de la producción de patulina. Determinados patrones de DNA pueden ser útiles para detectar hongos potenciales productores de patulina. La obtención de múltiples bandas en una PCR de muestras ambientales no cultivadas puede indicar que más de una especie está presente.Fundação para a Ciência e a Tecnologia (FCT).Asociación Española de Micología (AEM)Universidade do MinhoPaterson, R. R. M.20072007-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/7738eng"Revista Iberoamericana de Micología". ISSN 1130-1406. 24:4 (2007) 289-293.1130-140618095762http://www.reviberoammicol.com/2008-25/issue1.shtmlinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:44:18Zoai:repositorium.sdum.uminho.pt:1822/7738Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:41:57.717842Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The isoepoxydon dehydrogenase gene PCR profile is useful in fungal taxonomy |
title |
The isoepoxydon dehydrogenase gene PCR profile is useful in fungal taxonomy |
spellingShingle |
The isoepoxydon dehydrogenase gene PCR profile is useful in fungal taxonomy Paterson, R. R. M. Patulin PCR Numerical analysis Penicillium Aspergillus Byssochlamys Gen de la isoepoxydon deshidrogenasa Isoepoxydon dehydrogenase gene Patulina Análisis numérico |
title_short |
The isoepoxydon dehydrogenase gene PCR profile is useful in fungal taxonomy |
title_full |
The isoepoxydon dehydrogenase gene PCR profile is useful in fungal taxonomy |
title_fullStr |
The isoepoxydon dehydrogenase gene PCR profile is useful in fungal taxonomy |
title_full_unstemmed |
The isoepoxydon dehydrogenase gene PCR profile is useful in fungal taxonomy |
title_sort |
The isoepoxydon dehydrogenase gene PCR profile is useful in fungal taxonomy |
author |
Paterson, R. R. M. |
author_facet |
Paterson, R. R. M. |
author_role |
author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Paterson, R. R. M. |
dc.subject.por.fl_str_mv |
Patulin PCR Numerical analysis Penicillium Aspergillus Byssochlamys Gen de la isoepoxydon deshidrogenasa Isoepoxydon dehydrogenase gene Patulina Análisis numérico |
topic |
Patulin PCR Numerical analysis Penicillium Aspergillus Byssochlamys Gen de la isoepoxydon deshidrogenasa Isoepoxydon dehydrogenase gene Patulina Análisis numérico |
description |
This study evaluates the specificity of PCR isoepoxydon dehydrogenase (idh) primers on fungi associated with patulin production. The DNAs of 93 strains were extracted and analysed by PCR using primers of the idh gene of patulin biosynthesis. A single band at 620 bp was obtained on 17% of the analysed strains. Different molecular weight amplicons were observed in other strains. These were employed as binary characters for numerical analysis to obtain a dendrogram. Clusters were observed, which corresponded to morphological identifications in some cases. Amplicons at 400 and/or 500 bp were related to patulin non-detection for strains, whereas a 450 bp amplicon was associated with some Aspergillus and both of the Byssochlamys nivea strains tested. Hence, the idh primers are not specific for the gene and provide other amplicon products in other species. These results were useful providing (a) profiles of DNA to identify and classify fungi and (b) insights into patulin production. The DNA profiles in this study may be useful for determining patulin producing fungi. Obtaining multiple bands in culture-independent PCR of environmental samples by using the primers could indicate that more than one species is present. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007 2007-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/7738 |
url |
http://hdl.handle.net/1822/7738 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Revista Iberoamericana de Micología". ISSN 1130-1406. 24:4 (2007) 289-293. 1130-1406 18095762 http://www.reviberoammicol.com/2008-25/issue1.shtml |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Asociación Española de Micología (AEM) |
publisher.none.fl_str_mv |
Asociación Española de Micología (AEM) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132970363125760 |