The antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathway
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/7803 |
Resumo: | Ethnopharmacological relevance: Astragali Radix (AR) is the dry root of the leguminous plants Astragalus membranaceus (Fisch) Beg. var. mongholicus (Beg) Hsiao, and Astragalus membranaceus (Fisch) Bge., being used as a medicinal and edible resource. AR is used in traditional Chinese medicine prescriptions to treat hyperuricemia, but this particular effect is rarely reported, and the associated mechanism of action is still need to be elucidated.Aim of the study: To research the uric acid (UA)-lowering activity and mechanism of AR and the representative compounds through the constructed hyperuricemia mouse and cellular models.Materials and methods: In our study, the chemical profile of AR was analysed by UHPLC-QE-MS, as well as the mechanism of action of AR and the representative compounds on hyperuricemia was studied through the constructed hyperuricemia mouse and cellular models.Results: The main compounds in AR were terpenoids, flavonoids and alkaloids. Mice group treated with the highest AR dosage showed significantly lower (p < 0.0001) serum uric acid (208 & PLUSMN; 9 & mu;mol/L) than the control group (317 & PLUSMN; 11 & mu;mol/L). Furthermore, UA increased in a dose-dependence manner in urine and faeces. Serum creatinine and blood urea nitrogen standards, as well as xanthine oxidase in mice liver, decreased (p < 0.05) in all cases, indicating that AR could relieve acute hyperuricemia. UA reabsorption protein (URAT1 and GLUT9) was down-regulated in AR administration groups, while the secretory protein (ABCG2) was up-regulated, indicating that AR could promote the excretion of UA by regulating UA transporters via PI3K/Akt signalling pathway.Conclusion: This study validated the activity, and revealed the mechanism of AR in reducing UA, which provided experimental and clinical basis for the treatment of hyperuricemia with it. |
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The antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathwayAstragali RadixHyperuricemiaUA TransporterMechanismEthnopharmacological relevance: Astragali Radix (AR) is the dry root of the leguminous plants Astragalus membranaceus (Fisch) Beg. var. mongholicus (Beg) Hsiao, and Astragalus membranaceus (Fisch) Bge., being used as a medicinal and edible resource. AR is used in traditional Chinese medicine prescriptions to treat hyperuricemia, but this particular effect is rarely reported, and the associated mechanism of action is still need to be elucidated.Aim of the study: To research the uric acid (UA)-lowering activity and mechanism of AR and the representative compounds through the constructed hyperuricemia mouse and cellular models.Materials and methods: In our study, the chemical profile of AR was analysed by UHPLC-QE-MS, as well as the mechanism of action of AR and the representative compounds on hyperuricemia was studied through the constructed hyperuricemia mouse and cellular models.Results: The main compounds in AR were terpenoids, flavonoids and alkaloids. Mice group treated with the highest AR dosage showed significantly lower (p < 0.0001) serum uric acid (208 & PLUSMN; 9 & mu;mol/L) than the control group (317 & PLUSMN; 11 & mu;mol/L). Furthermore, UA increased in a dose-dependence manner in urine and faeces. Serum creatinine and blood urea nitrogen standards, as well as xanthine oxidase in mice liver, decreased (p < 0.05) in all cases, indicating that AR could relieve acute hyperuricemia. UA reabsorption protein (URAT1 and GLUT9) was down-regulated in AR administration groups, while the secretory protein (ABCG2) was up-regulated, indicating that AR could promote the excretion of UA by regulating UA transporters via PI3K/Akt signalling pathway.Conclusion: This study validated the activity, and revealed the mechanism of AR in reducing UA, which provided experimental and clinical basis for the treatment of hyperuricemia with it.This work was supported by the Central Guidance on Local Science and Technology Development Fund of Shandong, China (No. YDZX2022175; YDZX2022087); the Innovation Ability Improvement Project for Technology-Based Small and Medium-sized Enterprises of Shandong, China (No. 2022TSGC2002, 2022TSGC2065); the Provincial Major Scientific and Technological Innovation Project of Shandong, China (No. 2021SFGC0904, 2021TZXD001, 2022TZXD0029, 2022TZXD0032), the Natural Science Foundation of Shandong, China (No. ZR2020QD103; ZR2020MH401; ZR2021QH351).ElsevierBiblioteca Digital do IPBZhang, Meng-QiSun, Ke-XinGuo, XuChen, Ying-YingFeng, Cai-YunChen, Jia-ShuBarreira, João C.M.Prieto Lage, Miguel A.Sun, Jin-YueZhang, Jian-DongLi, NingyangLiu, Chao2013-01-03T16:58:24Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/7803engZhang, Meng-Qi; Sun, Ke-Xin; Guo, Xu; Chen, Ying-Ying; Feng, Cai-Yun; Chen, Jia-Shu; Barreira, João C.M.; Prieto Lage, Miguel A.; Sun, Jin-Yue; Zhang, Jian-Dong; Li, Ningyang; Liu, Chao (2023). The antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathway. Journal of Ethnopharmacology. eISSN 1872-7573. 317, p. 1-110378-874110.1016/j.jep.2023.1167701872-7573info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-10T01:18:41Zoai:bibliotecadigital.ipb.pt:10198/7803Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:19:55.986857Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathway |
title |
The antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathway |
spellingShingle |
The antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathway Zhang, Meng-Qi Astragali Radix Hyperuricemia UA Transporter Mechanism |
title_short |
The antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathway |
title_full |
The antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathway |
title_fullStr |
The antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathway |
title_full_unstemmed |
The antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathway |
title_sort |
The antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathway |
author |
Zhang, Meng-Qi |
author_facet |
Zhang, Meng-Qi Sun, Ke-Xin Guo, Xu Chen, Ying-Ying Feng, Cai-Yun Chen, Jia-Shu Barreira, João C.M. Prieto Lage, Miguel A. Sun, Jin-Yue Zhang, Jian-Dong Li, Ningyang Liu, Chao |
author_role |
author |
author2 |
Sun, Ke-Xin Guo, Xu Chen, Ying-Ying Feng, Cai-Yun Chen, Jia-Shu Barreira, João C.M. Prieto Lage, Miguel A. Sun, Jin-Yue Zhang, Jian-Dong Li, Ningyang Liu, Chao |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Zhang, Meng-Qi Sun, Ke-Xin Guo, Xu Chen, Ying-Ying Feng, Cai-Yun Chen, Jia-Shu Barreira, João C.M. Prieto Lage, Miguel A. Sun, Jin-Yue Zhang, Jian-Dong Li, Ningyang Liu, Chao |
dc.subject.por.fl_str_mv |
Astragali Radix Hyperuricemia UA Transporter Mechanism |
topic |
Astragali Radix Hyperuricemia UA Transporter Mechanism |
description |
Ethnopharmacological relevance: Astragali Radix (AR) is the dry root of the leguminous plants Astragalus membranaceus (Fisch) Beg. var. mongholicus (Beg) Hsiao, and Astragalus membranaceus (Fisch) Bge., being used as a medicinal and edible resource. AR is used in traditional Chinese medicine prescriptions to treat hyperuricemia, but this particular effect is rarely reported, and the associated mechanism of action is still need to be elucidated.Aim of the study: To research the uric acid (UA)-lowering activity and mechanism of AR and the representative compounds through the constructed hyperuricemia mouse and cellular models.Materials and methods: In our study, the chemical profile of AR was analysed by UHPLC-QE-MS, as well as the mechanism of action of AR and the representative compounds on hyperuricemia was studied through the constructed hyperuricemia mouse and cellular models.Results: The main compounds in AR were terpenoids, flavonoids and alkaloids. Mice group treated with the highest AR dosage showed significantly lower (p < 0.0001) serum uric acid (208 & PLUSMN; 9 & mu;mol/L) than the control group (317 & PLUSMN; 11 & mu;mol/L). Furthermore, UA increased in a dose-dependence manner in urine and faeces. Serum creatinine and blood urea nitrogen standards, as well as xanthine oxidase in mice liver, decreased (p < 0.05) in all cases, indicating that AR could relieve acute hyperuricemia. UA reabsorption protein (URAT1 and GLUT9) was down-regulated in AR administration groups, while the secretory protein (ABCG2) was up-regulated, indicating that AR could promote the excretion of UA by regulating UA transporters via PI3K/Akt signalling pathway.Conclusion: This study validated the activity, and revealed the mechanism of AR in reducing UA, which provided experimental and clinical basis for the treatment of hyperuricemia with it. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-03T16:58:24Z 2023 2023-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/7803 |
url |
http://hdl.handle.net/10198/7803 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Zhang, Meng-Qi; Sun, Ke-Xin; Guo, Xu; Chen, Ying-Ying; Feng, Cai-Yun; Chen, Jia-Shu; Barreira, João C.M.; Prieto Lage, Miguel A.; Sun, Jin-Yue; Zhang, Jian-Dong; Li, Ningyang; Liu, Chao (2023). The antihyperuricemia activity of Astragali Radix through regulating the expression of uric acid transporters via PI3K/Akt signalling pathway. Journal of Ethnopharmacology. eISSN 1872-7573. 317, p. 1-11 0378-8741 10.1016/j.jep.2023.116770 1872-7573 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799135498347741184 |