Characterization of a MOB1 homolog in the Apicomplexan parasite toxoplasma gondii

Detalhes bibliográficos
Autor(a) principal: Delgado, Inês L. S.
Data de Publicação: 2021
Outros Autores: Tavares, Alexandra, Francisco, Samuel, Santos, Dulce, Coelho, João, Basto, Afonso P., Zúquete, Sara, Müller, Joachim, Hemphill, Andrew, Meissner, Markus, Soares, Helena, Leitão, Alexandre, Nolasco, S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/14064
Resumo: FCT_UIDB/00100/2020. FCT_UIDP/00100/2020.
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spelling Characterization of a MOB1 homolog in the Apicomplexan parasite toxoplasma gondiiToxoplasma gondiiTachyzoiteMOB1Hippo pathwayMitotic exit networkApicomplexanEXPL/CVT-EPI/1945/2013FCT_UIDB/00100/2020FCT_UIDP/00100/2020FCT_UIDB/00100/2020. FCT_UIDP/00100/2020.Monopolar spindle One Binder1 (MOB1) proteins are conserved components of the tumor-suppressing Hippo pathway, regulating cellular processes such as cytokinesis. Apicomplexan parasites present a life cycle that relies on the parasites’ ability to differentiate between stages and regulate their proliferation; thus, Hippo signaling pathways could play an important role in the regulation of the apicomplexan life cycle. Here, we report the identification of one MOB1 protein in the apicomplexan Toxoplasma gondii. To characterize the function of MOB1, we generated gain-of-function transgenic lines with a ligand-controlled destabilization domain, and loss-of-function clonal lines obtained through CRISPR/Cas9 technology. Contrary to what has been characterized in other eukaryotes, MOB1 is not essential for cytokinesis in T. gondii. However, this picture is complex since we found MOB1 localized between the newly individualized daughter nuclei at the end of mitosis. Moreover, we detected a significant delay in the replication of overexpressing tachyzoites, contrasting with increased replication rates in knockout tachyzoites. Finally, using the proximity-biotinylation method, BioID, we identified novel members of the MOB1 interactome, a probable consequence of the observed lack of conservation of some key amino acid residues. Altogether, the results point to a complex evolutionary history of MOB1 roles in apicomplexans, sharing properties with other eukaryotes but also with divergent features, possibly associated with their complex life cycle.EXPL/CVT-EPI/1945/2013MDPIRCIPLDelgado, Inês L. S.Tavares, AlexandraFrancisco, SamuelSantos, DulceCoelho, JoãoBasto, Afonso P.Zúquete, SaraMüller, JoachimHemphill, AndrewMeissner, MarkusSoares, HelenaLeitão, AlexandreNolasco, S.2021-12-16T15:33:34Z2021-112021-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/14064engDelgado IL, Tavares A, Francisco S, Santos D, Soares H, Nolasco S, et al. Characterization of a MOB1 homolog in the Apicomplexan parasite toxoplasma gondii. Biology. 2021;10(12):1233.10.3390/biology10121233info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:09:44Zoai:repositorio.ipl.pt:10400.21/14064Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:21:56.284448Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Characterization of a MOB1 homolog in the Apicomplexan parasite toxoplasma gondii
title Characterization of a MOB1 homolog in the Apicomplexan parasite toxoplasma gondii
spellingShingle Characterization of a MOB1 homolog in the Apicomplexan parasite toxoplasma gondii
Delgado, Inês L. S.
Toxoplasma gondii
Tachyzoite
MOB1
Hippo pathway
Mitotic exit network
Apicomplexan
EXPL/CVT-EPI/1945/2013
FCT_UIDB/00100/2020
FCT_UIDP/00100/2020
title_short Characterization of a MOB1 homolog in the Apicomplexan parasite toxoplasma gondii
title_full Characterization of a MOB1 homolog in the Apicomplexan parasite toxoplasma gondii
title_fullStr Characterization of a MOB1 homolog in the Apicomplexan parasite toxoplasma gondii
title_full_unstemmed Characterization of a MOB1 homolog in the Apicomplexan parasite toxoplasma gondii
title_sort Characterization of a MOB1 homolog in the Apicomplexan parasite toxoplasma gondii
author Delgado, Inês L. S.
author_facet Delgado, Inês L. S.
Tavares, Alexandra
Francisco, Samuel
Santos, Dulce
Coelho, João
Basto, Afonso P.
Zúquete, Sara
Müller, Joachim
Hemphill, Andrew
Meissner, Markus
Soares, Helena
Leitão, Alexandre
Nolasco, S.
author_role author
author2 Tavares, Alexandra
Francisco, Samuel
Santos, Dulce
Coelho, João
Basto, Afonso P.
Zúquete, Sara
Müller, Joachim
Hemphill, Andrew
Meissner, Markus
Soares, Helena
Leitão, Alexandre
Nolasco, S.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Delgado, Inês L. S.
Tavares, Alexandra
Francisco, Samuel
Santos, Dulce
Coelho, João
Basto, Afonso P.
Zúquete, Sara
Müller, Joachim
Hemphill, Andrew
Meissner, Markus
Soares, Helena
Leitão, Alexandre
Nolasco, S.
dc.subject.por.fl_str_mv Toxoplasma gondii
Tachyzoite
MOB1
Hippo pathway
Mitotic exit network
Apicomplexan
EXPL/CVT-EPI/1945/2013
FCT_UIDB/00100/2020
FCT_UIDP/00100/2020
topic Toxoplasma gondii
Tachyzoite
MOB1
Hippo pathway
Mitotic exit network
Apicomplexan
EXPL/CVT-EPI/1945/2013
FCT_UIDB/00100/2020
FCT_UIDP/00100/2020
description FCT_UIDB/00100/2020. FCT_UIDP/00100/2020.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-16T15:33:34Z
2021-11
2021-11-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/14064
url http://hdl.handle.net/10400.21/14064
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Delgado IL, Tavares A, Francisco S, Santos D, Soares H, Nolasco S, et al. Characterization of a MOB1 homolog in the Apicomplexan parasite toxoplasma gondii. Biology. 2021;10(12):1233.
10.3390/biology10121233
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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