Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.21/14382 |
Resumo: | FCT_UID/Multi/00612/2019. FCT_UIDB/00100/2020. FCT_UIDP/00100/2020. |
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oai:repositorio.ipl.pt:10400.21/14382 |
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RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinomaAntineoplastic agentsTherapeutic useDrug delivery systemsLiposomesLung neoplasmsDrug therapyOligopeptidesSmall cell lung carcinomaTissue distributionFCT_UID/Multi/00612/2019FCT_UIDB/00100/2020FCT_UIDP/00100/2020FCT_UID/Multi/00612/2019. FCT_UIDB/00100/2020. FCT_UIDP/00100/2020.Ligand-mediated targeted liposomes have the potential to increase the therapeutic efficacy of anticancer drugs. This work aimed to evaluate the ability of antagonist G, a peptide targeting agent capable of blocking the action of multiple neuropeptides, to selectivity improve targeting and internalization of liposomal formulations (long-circulating liposomes, LCL, and stabilized antisense lipid particles containing ionizable amino lipid, SALP) to H69 and H82 small cell lung carcinoma (SCLC) cell lines. Antagonist G-targeted LCL and SALP were prepared by two different methods (either by direct covalent linkage at activated PEG grafted onto the liposomal surface or by post-insertion of DSPE-PEG-antagonist-G-conjugates into pre-formed liposomes). Association of the liposomal formulations with target SCLC cells was studied by fluorescence microscopy using fluorescence-labeled liposomes and confirmed quantitatively with [3H]-CHE-labelled liposomes. An antisense oligodeoxynucleotide against the overexpressed oncogene c-myc(as(c-myc)) was efficiently loaded into SALP formulations, the encapsulation efficiency decreased due to the inclusion of the targeting ligand. Also, liposome size was affected by as(c-myc) physical-chemical properties. The amount of antagonist G linked to the surface of the liposomal formulations was dependent on the coupling method and lipid composition used. Covalent attachment of antagonist G increased liposomes' cellular association and internalization via receptor-mediated and clathrin-dependent endocytosis, as assessed in SCLC cell lines. Biodistribution studies in healthy mice revealed a preferential lung accumulation of antagonist G-targeted SALP as compared to the non-targeted counterpart. Lung levels of the former were up to 3-fold higher 24 h after administration, highlighting their potential to be used as delivery vectors for SCLC treatment.ElsevierRCIPLCarvalheiro, ManuelaFerreira-Silva, MargaridaHolovanchuk, DenysMarinho, H. SusanaMoreira, João NunoSoares, HelenaCorvo, M. LuisaCruz, Maria Eugénia M.2024-03-01T01:30:21Z2022-012022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/14382engCarvalheiro M, Ferreira-Silva M, Holovanchuk D, Marinho S, Moreira JN, Soares H, et al. Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma. Int J Pharmaceutics. 2022;612:121380.10.1016/j.ijpharm.2021.121380info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-06T02:15:56Zoai:repositorio.ipl.pt:10400.21/14382Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:22:11.901657Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma |
title |
Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma |
spellingShingle |
Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma Carvalheiro, Manuela Antineoplastic agents Therapeutic use Drug delivery systems Liposomes Lung neoplasms Drug therapy Oligopeptides Small cell lung carcinoma Tissue distribution FCT_UID/Multi/00612/2019 FCT_UIDB/00100/2020 FCT_UIDP/00100/2020 |
title_short |
Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma |
title_full |
Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma |
title_fullStr |
Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma |
title_full_unstemmed |
Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma |
title_sort |
Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma |
author |
Carvalheiro, Manuela |
author_facet |
Carvalheiro, Manuela Ferreira-Silva, Margarida Holovanchuk, Denys Marinho, H. Susana Moreira, João Nuno Soares, Helena Corvo, M. Luisa Cruz, Maria Eugénia M. |
author_role |
author |
author2 |
Ferreira-Silva, Margarida Holovanchuk, Denys Marinho, H. Susana Moreira, João Nuno Soares, Helena Corvo, M. Luisa Cruz, Maria Eugénia M. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
RCIPL |
dc.contributor.author.fl_str_mv |
Carvalheiro, Manuela Ferreira-Silva, Margarida Holovanchuk, Denys Marinho, H. Susana Moreira, João Nuno Soares, Helena Corvo, M. Luisa Cruz, Maria Eugénia M. |
dc.subject.por.fl_str_mv |
Antineoplastic agents Therapeutic use Drug delivery systems Liposomes Lung neoplasms Drug therapy Oligopeptides Small cell lung carcinoma Tissue distribution FCT_UID/Multi/00612/2019 FCT_UIDB/00100/2020 FCT_UIDP/00100/2020 |
topic |
Antineoplastic agents Therapeutic use Drug delivery systems Liposomes Lung neoplasms Drug therapy Oligopeptides Small cell lung carcinoma Tissue distribution FCT_UID/Multi/00612/2019 FCT_UIDB/00100/2020 FCT_UIDP/00100/2020 |
description |
FCT_UID/Multi/00612/2019. FCT_UIDB/00100/2020. FCT_UIDP/00100/2020. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01 2022-01-01T00:00:00Z 2024-03-01T01:30:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.21/14382 |
url |
http://hdl.handle.net/10400.21/14382 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Carvalheiro M, Ferreira-Silva M, Holovanchuk D, Marinho S, Moreira JN, Soares H, et al. Antagonist G-targeted liposomes for improved delivery of anticancer drugs in small cell lung carcinoma. Int J Pharmaceutics. 2022;612:121380. 10.1016/j.ijpharm.2021.121380 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799133493817507840 |