Diversity and pathogenicity of pestalotioid fungi species associated with blueberry plants in Portugal
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/31931 |
Resumo: | Blueberry (Vaccinium corymbosum) is a perennial shrub, native to North America whose popularity has increasing due to its health benefits. In Portugal, this culture represents an important part of the country's economy, being cultivated in several regions. However, the fast growth of production has been accompanied by the appearance of pathogens, mainly fungi. Considering that studies carried out on pathogenic blueberry fungi in Portugal are still quite scarce, this study aimed to identify species of the genera Pestalotiopsis and Neopestalotiopsis, which are known pathogens severely affecting blueberry plantations worldwide. Therefore, a collection of 51 isolates was obtained from symptomatic blueberry plants collected in different cultivation orchards in Portugal. In order to assess the genetic diversity of our collection, all isolates were subjected to MSP-PCR fingerprinting. According to the analysis of the genetic profiles, 16 representative isolates were chosen for a molecular identification based on the sequencing and analysis of the ITS region, which allowed to identify 2 different genera: Pestalotiopsis and Neopestalotiopsis. To better discriminate the isolates at species level, a multi-locus sequence analysis was performed using, in addition to the ITS region, the protein coding genes: translation elongation factor 1-alpha (tef1-α) and beta-tubulin 2 (tub2). A phylogenetic analysis of the ITS, tef1-α and tub2 regions revealed the presence of 4 known species, placed into distinct clades (Pestalotiopsis chamaeropis, P. biciliata, P. australis and Neopestalotiopsis rosae), and 3 putative new species. These were characterized in terms of morphology and ability to grow at different temperatures and the names N. baccae, N. scalabiensis and N. vaccinii proposed. Of all the identified species, N. vaccinii and N. rosae were the most abundant. The pathogenicity tests carried out revealed that all tested species were pathogenic to blueberry plants (cultivar Duke). Among the species tested, P. biciliata and N. rosae were the most aggressive ones. Plants inoculated with isolates from these species exhibited more extensive branch necrosis and, in some cases, death of the plant. To our knowledge, this represents the first study and the first report of species of Pestalotiopsis and Neopestalotiopsis in blueberries in Portugal. The diversity and distribution of these species, as well as their pathogenic potential, need to be further explored in the future. |
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Diversity and pathogenicity of pestalotioid fungi species associated with blueberry plants in PortugalVaccinium corymbosumBlueberryPestalotiopsisNeopestalotiopsisTaxonomyPatogenicityBlueberry (Vaccinium corymbosum) is a perennial shrub, native to North America whose popularity has increasing due to its health benefits. In Portugal, this culture represents an important part of the country's economy, being cultivated in several regions. However, the fast growth of production has been accompanied by the appearance of pathogens, mainly fungi. Considering that studies carried out on pathogenic blueberry fungi in Portugal are still quite scarce, this study aimed to identify species of the genera Pestalotiopsis and Neopestalotiopsis, which are known pathogens severely affecting blueberry plantations worldwide. Therefore, a collection of 51 isolates was obtained from symptomatic blueberry plants collected in different cultivation orchards in Portugal. In order to assess the genetic diversity of our collection, all isolates were subjected to MSP-PCR fingerprinting. According to the analysis of the genetic profiles, 16 representative isolates were chosen for a molecular identification based on the sequencing and analysis of the ITS region, which allowed to identify 2 different genera: Pestalotiopsis and Neopestalotiopsis. To better discriminate the isolates at species level, a multi-locus sequence analysis was performed using, in addition to the ITS region, the protein coding genes: translation elongation factor 1-alpha (tef1-α) and beta-tubulin 2 (tub2). A phylogenetic analysis of the ITS, tef1-α and tub2 regions revealed the presence of 4 known species, placed into distinct clades (Pestalotiopsis chamaeropis, P. biciliata, P. australis and Neopestalotiopsis rosae), and 3 putative new species. These were characterized in terms of morphology and ability to grow at different temperatures and the names N. baccae, N. scalabiensis and N. vaccinii proposed. Of all the identified species, N. vaccinii and N. rosae were the most abundant. The pathogenicity tests carried out revealed that all tested species were pathogenic to blueberry plants (cultivar Duke). Among the species tested, P. biciliata and N. rosae were the most aggressive ones. Plants inoculated with isolates from these species exhibited more extensive branch necrosis and, in some cases, death of the plant. To our knowledge, this represents the first study and the first report of species of Pestalotiopsis and Neopestalotiopsis in blueberries in Portugal. The diversity and distribution of these species, as well as their pathogenic potential, need to be further explored in the future.O mirtilo (Vaccinium corymbosum) é um arbusto perene, nativo da América do Norte e que tem vindo a ganhar uma crescente popularidade devido aos seus benefícios para a saúde. Em Portugal, esta cultura representa uma parte importante para a economia do país, sendo cultivada em várias regiões. No entanto, o rápido crescimento da produção tem sido acompanhado pelo aparecimento de agentes patogénicos, essencialmente fungos. Tendo em conta que os estudos desenvolvidos sobre fungos patogénicos de mirtilo em Portugal ainda são bastante escassos, este estudo visa destacar as espécies dos géneros Pestalotiopsis e Neopestalotiopsis, reconhecidos como agentes patogénicos que podem afetar severamente as plantações de mirtilo (entre variadíssimas colheitas) primando pela sua identificação e avaliação do seu perfil de patogenicidade. Por conseguinte, foi obtida uma coleção de 51 isolados a partir de plantas de mirtilo sintomáticas recolhidas em diferentes pomares em Portugal. Para avaliar a diversidade genética da coleção, todos os isolados foram inicialmente submetidos a tipagem por MSP-PCR. Segundo a análise dos perfis genéticos, foram selecionados 16 isolados para uma identificação molecular com base na sequenciação e análise da região ITS, o que permitiu dividir os isolados em 2 géneros diferentes: Pestalotiopsis e Neopestalotiopsis. Para melhor discriminar os isolados ao nível da espécie, foi realizada uma análise multilocus utilizando, para além da região ITS, as sequências das seguintes regiões – fator de alongamento da transcrição 1-alfa (tef1-α) e beta-tubulina 2 (tub2). A análise filogenética das regiões ITS, tef1-α e tub2, revelou a presença de 4 espécies conhecidas (Pestalotiopsis chamaeropis, P. biciliata, P. australis e Neopestalotiopsis rosae), e 3 potenciais espécies novas. Estas últimas foram caracterizadas em termos morfológicos e capacidade de crescimento a diferentes temperaturas de incubação sendo propostos os nomes N. baccae, N. scalabiensis e N. vaccinii. De todas estas espécies identificadas, N. vaccinii e N. rosae foram as mais abundantes. Os testes de patogenicidade realizados revelaram que todas as espécies testadas mostraram ser patogénicas para plantas de mirtilo (cultivar Duke). Entre as espécies testadas, P. biciliata e N. rosae mostraram ser as mais agressivas. As plantas inoculadas com isolados destas espécies induziram necroses mais extensas nos caules e, em alguns casos, morte da planta. Estes resultados representam o primeiro estudo de identificação de espécies de Pestalotiopsis e Neopestalotiopsis em mirtilo em Portugal. A diversidade e distribuição destas espécies, bem como o seu potencial patogénico, precisam de ser melhor estudadas no futuro.2022-08-02T00:00:00Z2021-07-21T00:00:00Z2021-07-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/31931engSantos, João Diogo Fernandesinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-06T04:33:19Zoai:ria.ua.pt:10773/31931Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-06T04:33:19Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Diversity and pathogenicity of pestalotioid fungi species associated with blueberry plants in Portugal |
title |
Diversity and pathogenicity of pestalotioid fungi species associated with blueberry plants in Portugal |
spellingShingle |
Diversity and pathogenicity of pestalotioid fungi species associated with blueberry plants in Portugal Santos, João Diogo Fernandes Vaccinium corymbosum Blueberry Pestalotiopsis Neopestalotiopsis Taxonomy Patogenicity |
title_short |
Diversity and pathogenicity of pestalotioid fungi species associated with blueberry plants in Portugal |
title_full |
Diversity and pathogenicity of pestalotioid fungi species associated with blueberry plants in Portugal |
title_fullStr |
Diversity and pathogenicity of pestalotioid fungi species associated with blueberry plants in Portugal |
title_full_unstemmed |
Diversity and pathogenicity of pestalotioid fungi species associated with blueberry plants in Portugal |
title_sort |
Diversity and pathogenicity of pestalotioid fungi species associated with blueberry plants in Portugal |
author |
Santos, João Diogo Fernandes |
author_facet |
Santos, João Diogo Fernandes |
author_role |
author |
dc.contributor.author.fl_str_mv |
Santos, João Diogo Fernandes |
dc.subject.por.fl_str_mv |
Vaccinium corymbosum Blueberry Pestalotiopsis Neopestalotiopsis Taxonomy Patogenicity |
topic |
Vaccinium corymbosum Blueberry Pestalotiopsis Neopestalotiopsis Taxonomy Patogenicity |
description |
Blueberry (Vaccinium corymbosum) is a perennial shrub, native to North America whose popularity has increasing due to its health benefits. In Portugal, this culture represents an important part of the country's economy, being cultivated in several regions. However, the fast growth of production has been accompanied by the appearance of pathogens, mainly fungi. Considering that studies carried out on pathogenic blueberry fungi in Portugal are still quite scarce, this study aimed to identify species of the genera Pestalotiopsis and Neopestalotiopsis, which are known pathogens severely affecting blueberry plantations worldwide. Therefore, a collection of 51 isolates was obtained from symptomatic blueberry plants collected in different cultivation orchards in Portugal. In order to assess the genetic diversity of our collection, all isolates were subjected to MSP-PCR fingerprinting. According to the analysis of the genetic profiles, 16 representative isolates were chosen for a molecular identification based on the sequencing and analysis of the ITS region, which allowed to identify 2 different genera: Pestalotiopsis and Neopestalotiopsis. To better discriminate the isolates at species level, a multi-locus sequence analysis was performed using, in addition to the ITS region, the protein coding genes: translation elongation factor 1-alpha (tef1-α) and beta-tubulin 2 (tub2). A phylogenetic analysis of the ITS, tef1-α and tub2 regions revealed the presence of 4 known species, placed into distinct clades (Pestalotiopsis chamaeropis, P. biciliata, P. australis and Neopestalotiopsis rosae), and 3 putative new species. These were characterized in terms of morphology and ability to grow at different temperatures and the names N. baccae, N. scalabiensis and N. vaccinii proposed. Of all the identified species, N. vaccinii and N. rosae were the most abundant. The pathogenicity tests carried out revealed that all tested species were pathogenic to blueberry plants (cultivar Duke). Among the species tested, P. biciliata and N. rosae were the most aggressive ones. Plants inoculated with isolates from these species exhibited more extensive branch necrosis and, in some cases, death of the plant. To our knowledge, this represents the first study and the first report of species of Pestalotiopsis and Neopestalotiopsis in blueberries in Portugal. The diversity and distribution of these species, as well as their pathogenic potential, need to be further explored in the future. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-21T00:00:00Z 2021-07-21 2022-08-02T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/31931 |
url |
http://hdl.handle.net/10773/31931 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
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embargoedAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817543788100321280 |