A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/68440 |
Resumo: | A simple device which incorporates three electrodes (working electrode, counter electrode and reference electrode) was constructed to be used currently in laboratories without elevated cost. It does not need more than 2 μL of electrolyte, sample or working solution, his support material is paper, and the working electrode which is based on carbon ink can incorporate enzymes and cofactors. To test this concept we started this investigation using the NADH/NAD+ redox couple which is an omnipresent coenzyme in living systems but is also a challenge to electrochemistry. The paper sensor fabrication was simple, rapid and cheaper. NADH was incorporated in the carbon ink by mixing both and, this mixture was used to print the working electrode. The direct electrochemical system NADH/NAD+ signal obtained, using this device, appeared at low potentials. A quasi-reversible diffusional redox process was achieved and regeneration of the NADH after oxidation was reached. This small paper device was not only used to study the redox process of NAD+/NADH, but also its behavior in the presence of electroactive (ascorbic acid) and non-eletroactive species (glucose). |
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A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox SystemNADH/NAD+Screen-printing biosensorPaper deviceA simple device which incorporates three electrodes (working electrode, counter electrode and reference electrode) was constructed to be used currently in laboratories without elevated cost. It does not need more than 2 μL of electrolyte, sample or working solution, his support material is paper, and the working electrode which is based on carbon ink can incorporate enzymes and cofactors. To test this concept we started this investigation using the NADH/NAD+ redox couple which is an omnipresent coenzyme in living systems but is also a challenge to electrochemistry. The paper sensor fabrication was simple, rapid and cheaper. NADH was incorporated in the carbon ink by mixing both and, this mixture was used to print the working electrode. The direct electrochemical system NADH/NAD+ signal obtained, using this device, appeared at low potentials. A quasi-reversible diffusional redox process was achieved and regeneration of the NADH after oxidation was reached. This small paper device was not only used to study the redox process of NAD+/NADH, but also its behavior in the presence of electroactive (ascorbic acid) and non-eletroactive species (glucose).DCM - Departamento de Ciência dos MateriaisRUNCoutinho, Isabel Cristina Ribau FernandesFortunato, E.2019-05-02T22:18:57Z2018-02-282018-02-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/68440eng0022-3573PURE: 12707439https://doi.org/10.17265/2328-2150/2018.02.011info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:32:20Zoai:run.unl.pt:10362/68440Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:34:46.352347Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System |
title |
A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System |
spellingShingle |
A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System Coutinho, Isabel Cristina Ribau Fernandes NADH/NAD+ Screen-printing biosensor Paper device |
title_short |
A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System |
title_full |
A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System |
title_fullStr |
A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System |
title_full_unstemmed |
A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System |
title_sort |
A Simple Procedure to Fabricate Paper Biosensor and Its Applicability—NADH/NAD+ Redox System |
author |
Coutinho, Isabel Cristina Ribau Fernandes |
author_facet |
Coutinho, Isabel Cristina Ribau Fernandes Fortunato, E. |
author_role |
author |
author2 |
Fortunato, E. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
DCM - Departamento de Ciência dos Materiais RUN |
dc.contributor.author.fl_str_mv |
Coutinho, Isabel Cristina Ribau Fernandes Fortunato, E. |
dc.subject.por.fl_str_mv |
NADH/NAD+ Screen-printing biosensor Paper device |
topic |
NADH/NAD+ Screen-printing biosensor Paper device |
description |
A simple device which incorporates three electrodes (working electrode, counter electrode and reference electrode) was constructed to be used currently in laboratories without elevated cost. It does not need more than 2 μL of electrolyte, sample or working solution, his support material is paper, and the working electrode which is based on carbon ink can incorporate enzymes and cofactors. To test this concept we started this investigation using the NADH/NAD+ redox couple which is an omnipresent coenzyme in living systems but is also a challenge to electrochemistry. The paper sensor fabrication was simple, rapid and cheaper. NADH was incorporated in the carbon ink by mixing both and, this mixture was used to print the working electrode. The direct electrochemical system NADH/NAD+ signal obtained, using this device, appeared at low potentials. A quasi-reversible diffusional redox process was achieved and regeneration of the NADH after oxidation was reached. This small paper device was not only used to study the redox process of NAD+/NADH, but also its behavior in the presence of electroactive (ascorbic acid) and non-eletroactive species (glucose). |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-02-28 2018-02-28T00:00:00Z 2019-05-02T22:18:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/68440 |
url |
http://hdl.handle.net/10362/68440 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0022-3573 PURE: 12707439 https://doi.org/10.17265/2328-2150/2018.02.011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137969846616064 |