Rapid detection of Ganoderma-infected oil palms by microwave ergosterol extraction with HPLC and TLC

Detalhes bibliográficos
Autor(a) principal: Muniroh, M. S.
Data de Publicação: 2014
Outros Autores: Sariah, M., Abidin, M. A. Zainal, Lima, Nelson, Paterson, R. R. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/31582
Resumo: Detection of basal stem rot (BSR) by Ganoderma of oil palms was based on foliar symptoms and production of basidiomata. Enzyme-Linked Immunosorbent Assays-Polyclonal Antibody (ELISA-PAB) and PCR have been proposed as early detection methods for the disease. These techniques are complex, time consuming and have accuracy limitations. An ergosterol method was developed which correlated well with the degree of infection in oil palms, including samples growing in plantations. However, the method was capable of being optimised. This current study was designed to develop a simpler, more rapid and efficient ergosterol method with utility in the field that involved the use of microwave extraction. The optimised procedure involved extracting a small amount of Ganoderma, or Ganoderma-infected oil palm suspended in low volumes of solvent followed by irradiation in a conventional microwave oven at 70 °C and medium high power for 30 s, resulting in simultaneous extraction and saponification. Ergosterol was detected by thin layer chromatography (TLC) and quantified using high performance liquid chromatography with diode array detection. The TLC method was novel and provided a simple, inexpensive method with utility in the field. The new method was particularly effective at extracting high yields of ergosterol from infected oil palm and enables rapid analysis of field samples on site, allowing infected oil palms to be treated or culled very rapidly. Some limitations of the method are discussed herein. The procedures lend themselves to controlling the disease more effectively and allowing more effective use of land currently employed to grow oil palms, thereby reducing pressure to develop new plantations.
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spelling Rapid detection of Ganoderma-infected oil palms by microwave ergosterol extraction with HPLC and TLCMicrowave assisted extractionGanodermaErgosterolOil palmTLCHPLCScience & TechnologyDetection of basal stem rot (BSR) by Ganoderma of oil palms was based on foliar symptoms and production of basidiomata. Enzyme-Linked Immunosorbent Assays-Polyclonal Antibody (ELISA-PAB) and PCR have been proposed as early detection methods for the disease. These techniques are complex, time consuming and have accuracy limitations. An ergosterol method was developed which correlated well with the degree of infection in oil palms, including samples growing in plantations. However, the method was capable of being optimised. This current study was designed to develop a simpler, more rapid and efficient ergosterol method with utility in the field that involved the use of microwave extraction. The optimised procedure involved extracting a small amount of Ganoderma, or Ganoderma-infected oil palm suspended in low volumes of solvent followed by irradiation in a conventional microwave oven at 70 °C and medium high power for 30 s, resulting in simultaneous extraction and saponification. Ergosterol was detected by thin layer chromatography (TLC) and quantified using high performance liquid chromatography with diode array detection. The TLC method was novel and provided a simple, inexpensive method with utility in the field. The new method was particularly effective at extracting high yields of ergosterol from infected oil palm and enables rapid analysis of field samples on site, allowing infected oil palms to be treated or culled very rapidly. Some limitations of the method are discussed herein. The procedures lend themselves to controlling the disease more effectively and allowing more effective use of land currently employed to grow oil palms, thereby reducing pressure to develop new plantations.This project was supported by the Fundamental Research Grant Scheme (FRGS), administered through the Ministry of Higher Education, Malaysia (Grant No: 5524175).ElsevierUniversidade do MinhoMuniroh, M. S.Sariah, M.Abidin, M. A. ZainalLima, NelsonPaterson, R. R. M.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31582eng0167-70120167-701210.1016/j.mimet.2014.03.00524681306www.elsevier.com/locate/jmicmethinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:36:17Zoai:repositorium.sdum.uminho.pt:1822/31582Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:32:19.599604Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Rapid detection of Ganoderma-infected oil palms by microwave ergosterol extraction with HPLC and TLC
title Rapid detection of Ganoderma-infected oil palms by microwave ergosterol extraction with HPLC and TLC
spellingShingle Rapid detection of Ganoderma-infected oil palms by microwave ergosterol extraction with HPLC and TLC
Muniroh, M. S.
Microwave assisted extraction
Ganoderma
Ergosterol
Oil palm
TLC
HPLC
Science & Technology
title_short Rapid detection of Ganoderma-infected oil palms by microwave ergosterol extraction with HPLC and TLC
title_full Rapid detection of Ganoderma-infected oil palms by microwave ergosterol extraction with HPLC and TLC
title_fullStr Rapid detection of Ganoderma-infected oil palms by microwave ergosterol extraction with HPLC and TLC
title_full_unstemmed Rapid detection of Ganoderma-infected oil palms by microwave ergosterol extraction with HPLC and TLC
title_sort Rapid detection of Ganoderma-infected oil palms by microwave ergosterol extraction with HPLC and TLC
author Muniroh, M. S.
author_facet Muniroh, M. S.
Sariah, M.
Abidin, M. A. Zainal
Lima, Nelson
Paterson, R. R. M.
author_role author
author2 Sariah, M.
Abidin, M. A. Zainal
Lima, Nelson
Paterson, R. R. M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Muniroh, M. S.
Sariah, M.
Abidin, M. A. Zainal
Lima, Nelson
Paterson, R. R. M.
dc.subject.por.fl_str_mv Microwave assisted extraction
Ganoderma
Ergosterol
Oil palm
TLC
HPLC
Science & Technology
topic Microwave assisted extraction
Ganoderma
Ergosterol
Oil palm
TLC
HPLC
Science & Technology
description Detection of basal stem rot (BSR) by Ganoderma of oil palms was based on foliar symptoms and production of basidiomata. Enzyme-Linked Immunosorbent Assays-Polyclonal Antibody (ELISA-PAB) and PCR have been proposed as early detection methods for the disease. These techniques are complex, time consuming and have accuracy limitations. An ergosterol method was developed which correlated well with the degree of infection in oil palms, including samples growing in plantations. However, the method was capable of being optimised. This current study was designed to develop a simpler, more rapid and efficient ergosterol method with utility in the field that involved the use of microwave extraction. The optimised procedure involved extracting a small amount of Ganoderma, or Ganoderma-infected oil palm suspended in low volumes of solvent followed by irradiation in a conventional microwave oven at 70 °C and medium high power for 30 s, resulting in simultaneous extraction and saponification. Ergosterol was detected by thin layer chromatography (TLC) and quantified using high performance liquid chromatography with diode array detection. The TLC method was novel and provided a simple, inexpensive method with utility in the field. The new method was particularly effective at extracting high yields of ergosterol from infected oil palm and enables rapid analysis of field samples on site, allowing infected oil palms to be treated or culled very rapidly. Some limitations of the method are discussed herein. The procedures lend themselves to controlling the disease more effectively and allowing more effective use of land currently employed to grow oil palms, thereby reducing pressure to develop new plantations.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/31582
url http://hdl.handle.net/1822/31582
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0167-7012
0167-7012
10.1016/j.mimet.2014.03.005
24681306
www.elsevier.com/locate/jmicmeth
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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