Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of the Brazilian Chemical Society (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000900012 |
Resumo: | Ergosterol was used as indicator of fungal contamination to assess mould presence on the building materials. Microwave-assisted saponification followed by pentane extraction was used in the sample preparation step. Samples of brick, plaster, concrete and granite were analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Ergosterol concentrations in laboratory infected samples ranged from 6 to 16 ng g-1, while in samples of naturally occurring materials ranged from 66 to 3020 ng g-1. Limits of detection and of quantitation were 1.7 and 5.5 ng g-1, respectively. Recovery and matrix effect were tested using samples spiked at 100 ng g-1. Mean recovery and mean matrix effect were 65.9 and 111.6%, respectively. Microscopic observations and bioluminescence measurements were carried out for the infected samples to make additional confirmation of fungi presence. The ergosterol concentration determined by HPLC-MS/MS in building materials proved to be a suitable indicator to estimate fungal contamination. The developed method was considerably faster for this aim than bioluminescence measurements and traditionally used culture methods. |
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Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materialsergosterol, building materialmicrowave extractionbioluminescence imagingErgosterol was used as indicator of fungal contamination to assess mould presence on the building materials. Microwave-assisted saponification followed by pentane extraction was used in the sample preparation step. Samples of brick, plaster, concrete and granite were analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Ergosterol concentrations in laboratory infected samples ranged from 6 to 16 ng g-1, while in samples of naturally occurring materials ranged from 66 to 3020 ng g-1. Limits of detection and of quantitation were 1.7 and 5.5 ng g-1, respectively. Recovery and matrix effect were tested using samples spiked at 100 ng g-1. Mean recovery and mean matrix effect were 65.9 and 111.6%, respectively. Microscopic observations and bioluminescence measurements were carried out for the infected samples to make additional confirmation of fungi presence. The ergosterol concentration determined by HPLC-MS/MS in building materials proved to be a suitable indicator to estimate fungal contamination. The developed method was considerably faster for this aim than bioluminescence measurements and traditionally used culture methods.Sociedade Brasileira de Química2013-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000900012Journal of the Brazilian Chemical Society v.24 n.9 2013reponame:Journal of the Brazilian Chemical Society (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.5935/0103-5053.20130191info:eu-repo/semantics/openAccessHorbik,DariaŁowińska-Kluge,AldonaGórski,ZbigniewStanisz,EwaZgoła-Grześkowiak,Agnieszkaeng2013-09-24T00:00:00Zoai:scielo:S0103-50532013000900012Revistahttp://jbcs.sbq.org.brONGhttps://old.scielo.br/oai/scielo-oai.php||office@jbcs.sbq.org.br1678-47900103-5053opendoar:2013-09-24T00:00Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ)false |
dc.title.none.fl_str_mv |
Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials |
title |
Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials |
spellingShingle |
Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials Horbik,Daria ergosterol, building material microwave extraction bioluminescence imaging |
title_short |
Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials |
title_full |
Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials |
title_fullStr |
Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials |
title_full_unstemmed |
Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials |
title_sort |
Microwave-assisted extraction combined with HPLC-MS/MS for diagnosis of fungal contamination in building materials |
author |
Horbik,Daria |
author_facet |
Horbik,Daria Łowińska-Kluge,Aldona Górski,Zbigniew Stanisz,Ewa Zgoła-Grześkowiak,Agnieszka |
author_role |
author |
author2 |
Łowińska-Kluge,Aldona Górski,Zbigniew Stanisz,Ewa Zgoła-Grześkowiak,Agnieszka |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Horbik,Daria Łowińska-Kluge,Aldona Górski,Zbigniew Stanisz,Ewa Zgoła-Grześkowiak,Agnieszka |
dc.subject.por.fl_str_mv |
ergosterol, building material microwave extraction bioluminescence imaging |
topic |
ergosterol, building material microwave extraction bioluminescence imaging |
description |
Ergosterol was used as indicator of fungal contamination to assess mould presence on the building materials. Microwave-assisted saponification followed by pentane extraction was used in the sample preparation step. Samples of brick, plaster, concrete and granite were analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Ergosterol concentrations in laboratory infected samples ranged from 6 to 16 ng g-1, while in samples of naturally occurring materials ranged from 66 to 3020 ng g-1. Limits of detection and of quantitation were 1.7 and 5.5 ng g-1, respectively. Recovery and matrix effect were tested using samples spiked at 100 ng g-1. Mean recovery and mean matrix effect were 65.9 and 111.6%, respectively. Microscopic observations and bioluminescence measurements were carried out for the infected samples to make additional confirmation of fungi presence. The ergosterol concentration determined by HPLC-MS/MS in building materials proved to be a suitable indicator to estimate fungal contamination. The developed method was considerably faster for this aim than bioluminescence measurements and traditionally used culture methods. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000900012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532013000900012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.5935/0103-5053.20130191 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
publisher.none.fl_str_mv |
Sociedade Brasileira de Química |
dc.source.none.fl_str_mv |
Journal of the Brazilian Chemical Society v.24 n.9 2013 reponame:Journal of the Brazilian Chemical Society (Online) instname:Sociedade Brasileira de Química (SBQ) instacron:SBQ |
instname_str |
Sociedade Brasileira de Química (SBQ) |
instacron_str |
SBQ |
institution |
SBQ |
reponame_str |
Journal of the Brazilian Chemical Society (Online) |
collection |
Journal of the Brazilian Chemical Society (Online) |
repository.name.fl_str_mv |
Journal of the Brazilian Chemical Society (Online) - Sociedade Brasileira de Química (SBQ) |
repository.mail.fl_str_mv |
||office@jbcs.sbq.org.br |
_version_ |
1750318175273091072 |