HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES

Detalhes bibliográficos
Autor(a) principal: Schenkman, Sergio [UNIFESP]
Data de Publicação: 1992
Outros Autores: Mortara, R. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://jcs.biologists.org/content/101/4/895
http://repositorio.unifesp.br/handle/11600/44231
Resumo: We show here that HeLa cell microfilaments can be stained by phatloidin at the sites of invasion of Trypanosoma cruzi trypomastigotes, one of the infective stages of this protozoan parasite. Concurrently, a projection of the HeLa cell plasmalemma encircles invading parasites. This plasmalemma projection is further internalized and entire membrane protrusions containing parasites are found within cytoplasmic vacuoles of the host cell. Neither the microfilament staining around invading parasites nor the plasma-lemma extension is inhibited by cytochalasin D, a drug that is unable to prevent trypomastigote entry into HeLa cells. The internalization of the membrane expansion, however, is blocked by the drug. These novel observations indicate that although the driving force for T. cruzi penetration comes from the parasite, the cortical target cytoskeleton of the target cell is concomitantly modified. The molecular characterization of this phenomenon may provide a new insight into the understanding of the mechanisms involved in the active penetration of T. cruzi into mammalian cells.
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spelling HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTESTRYPANOSOMA-CRUZIACTINMICROFILAMENTSHELA CELLSCYTOCHALASIN-DINVASIONPSEUDOPODIAMEMBRANEWe show here that HeLa cell microfilaments can be stained by phatloidin at the sites of invasion of Trypanosoma cruzi trypomastigotes, one of the infective stages of this protozoan parasite. Concurrently, a projection of the HeLa cell plasmalemma encircles invading parasites. This plasmalemma projection is further internalized and entire membrane protrusions containing parasites are found within cytoplasmic vacuoles of the host cell. Neither the microfilament staining around invading parasites nor the plasma-lemma extension is inhibited by cytochalasin D, a drug that is unable to prevent trypomastigote entry into HeLa cells. The internalization of the membrane expansion, however, is blocked by the drug. These novel observations indicate that although the driving force for T. cruzi penetration comes from the parasite, the cortical target cytoskeleton of the target cell is concomitantly modified. The molecular characterization of this phenomenon may provide a new insight into the understanding of the mechanisms involved in the active penetration of T. cruzi into mammalian cells.ESCOLA PAULISTA MED SCH,DEPT MICROBIOL IMMUNOL & PARASITOL,RUA BOTUCATU,862-6 ANDAR,BR-04023 SAO PAULO,BRAZILESCOLA PAULISTA MED SCH,DEPT MICROBIOL IMMUNOL & PARASITOL,RUA BOTUCATU,862-6 ANDAR,BR-04023 SAO PAULO,BRAZILWeb of ScienceCompany Of Biologists LtdUniversidade Federal de São Paulo (UNIFESP)Schenkman, Sergio [UNIFESP]Mortara, R. A.2018-06-15T17:53:09Z2018-06-15T17:53:09Z1992-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion895-905http://jcs.biologists.org/content/101/4/895Journal Of Cell Science. Cambridge: Company Of Biologists Ltd, v. 101, p. 895-905, 1992.0021-9533http://repositorio.unifesp.br/handle/11600/44231WOS:A1992HR16000016engJournal Of Cell Scienceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T11:58:49Zoai:repositorio.unifesp.br/:11600/44231Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T11:58:49Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES
title HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES
spellingShingle HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES
Schenkman, Sergio [UNIFESP]
TRYPANOSOMA-CRUZI
ACTIN
MICROFILAMENTS
HELA CELLS
CYTOCHALASIN-D
INVASION
PSEUDOPODIA
MEMBRANE
title_short HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES
title_full HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES
title_fullStr HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES
title_full_unstemmed HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES
title_sort HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES
author Schenkman, Sergio [UNIFESP]
author_facet Schenkman, Sergio [UNIFESP]
Mortara, R. A.
author_role author
author2 Mortara, R. A.
author2_role author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Schenkman, Sergio [UNIFESP]
Mortara, R. A.
dc.subject.por.fl_str_mv TRYPANOSOMA-CRUZI
ACTIN
MICROFILAMENTS
HELA CELLS
CYTOCHALASIN-D
INVASION
PSEUDOPODIA
MEMBRANE
topic TRYPANOSOMA-CRUZI
ACTIN
MICROFILAMENTS
HELA CELLS
CYTOCHALASIN-D
INVASION
PSEUDOPODIA
MEMBRANE
description We show here that HeLa cell microfilaments can be stained by phatloidin at the sites of invasion of Trypanosoma cruzi trypomastigotes, one of the infective stages of this protozoan parasite. Concurrently, a projection of the HeLa cell plasmalemma encircles invading parasites. This plasmalemma projection is further internalized and entire membrane protrusions containing parasites are found within cytoplasmic vacuoles of the host cell. Neither the microfilament staining around invading parasites nor the plasma-lemma extension is inhibited by cytochalasin D, a drug that is unable to prevent trypomastigote entry into HeLa cells. The internalization of the membrane expansion, however, is blocked by the drug. These novel observations indicate that although the driving force for T. cruzi penetration comes from the parasite, the cortical target cytoskeleton of the target cell is concomitantly modified. The molecular characterization of this phenomenon may provide a new insight into the understanding of the mechanisms involved in the active penetration of T. cruzi into mammalian cells.
publishDate 1992
dc.date.none.fl_str_mv 1992-04-01
2018-06-15T17:53:09Z
2018-06-15T17:53:09Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://jcs.biologists.org/content/101/4/895
Journal Of Cell Science. Cambridge: Company Of Biologists Ltd, v. 101, p. 895-905, 1992.
0021-9533
http://repositorio.unifesp.br/handle/11600/44231
WOS:A1992HR16000016
url http://jcs.biologists.org/content/101/4/895
http://repositorio.unifesp.br/handle/11600/44231
identifier_str_mv Journal Of Cell Science. Cambridge: Company Of Biologists Ltd, v. 101, p. 895-905, 1992.
0021-9533
WOS:A1992HR16000016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Cell Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 895-905
dc.publisher.none.fl_str_mv Company Of Biologists Ltd
publisher.none.fl_str_mv Company Of Biologists Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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