HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES
Autor(a) principal: | |
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Data de Publicação: | 1992 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNIFESP |
Texto Completo: | http://jcs.biologists.org/content/101/4/895 http://repositorio.unifesp.br/handle/11600/44231 |
Resumo: | We show here that HeLa cell microfilaments can be stained by phatloidin at the sites of invasion of Trypanosoma cruzi trypomastigotes, one of the infective stages of this protozoan parasite. Concurrently, a projection of the HeLa cell plasmalemma encircles invading parasites. This plasmalemma projection is further internalized and entire membrane protrusions containing parasites are found within cytoplasmic vacuoles of the host cell. Neither the microfilament staining around invading parasites nor the plasma-lemma extension is inhibited by cytochalasin D, a drug that is unable to prevent trypomastigote entry into HeLa cells. The internalization of the membrane expansion, however, is blocked by the drug. These novel observations indicate that although the driving force for T. cruzi penetration comes from the parasite, the cortical target cytoskeleton of the target cell is concomitantly modified. The molecular characterization of this phenomenon may provide a new insight into the understanding of the mechanisms involved in the active penetration of T. cruzi into mammalian cells. |
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HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTESTRYPANOSOMA-CRUZIACTINMICROFILAMENTSHELA CELLSCYTOCHALASIN-DINVASIONPSEUDOPODIAMEMBRANEWe show here that HeLa cell microfilaments can be stained by phatloidin at the sites of invasion of Trypanosoma cruzi trypomastigotes, one of the infective stages of this protozoan parasite. Concurrently, a projection of the HeLa cell plasmalemma encircles invading parasites. This plasmalemma projection is further internalized and entire membrane protrusions containing parasites are found within cytoplasmic vacuoles of the host cell. Neither the microfilament staining around invading parasites nor the plasma-lemma extension is inhibited by cytochalasin D, a drug that is unable to prevent trypomastigote entry into HeLa cells. The internalization of the membrane expansion, however, is blocked by the drug. These novel observations indicate that although the driving force for T. cruzi penetration comes from the parasite, the cortical target cytoskeleton of the target cell is concomitantly modified. The molecular characterization of this phenomenon may provide a new insight into the understanding of the mechanisms involved in the active penetration of T. cruzi into mammalian cells.ESCOLA PAULISTA MED SCH,DEPT MICROBIOL IMMUNOL & PARASITOL,RUA BOTUCATU,862-6 ANDAR,BR-04023 SAO PAULO,BRAZILESCOLA PAULISTA MED SCH,DEPT MICROBIOL IMMUNOL & PARASITOL,RUA BOTUCATU,862-6 ANDAR,BR-04023 SAO PAULO,BRAZILWeb of ScienceCompany Of Biologists LtdUniversidade Federal de São Paulo (UNIFESP)Schenkman, Sergio [UNIFESP]Mortara, R. A.2018-06-15T17:53:09Z2018-06-15T17:53:09Z1992-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion895-905http://jcs.biologists.org/content/101/4/895Journal Of Cell Science. Cambridge: Company Of Biologists Ltd, v. 101, p. 895-905, 1992.0021-9533http://repositorio.unifesp.br/handle/11600/44231WOS:A1992HR16000016engJournal Of Cell Scienceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-05-02T11:58:49Zoai:repositorio.unifesp.br/:11600/44231Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-05-02T11:58:49Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
dc.title.none.fl_str_mv |
HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES |
title |
HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES |
spellingShingle |
HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES Schenkman, Sergio [UNIFESP] TRYPANOSOMA-CRUZI ACTIN MICROFILAMENTS HELA CELLS CYTOCHALASIN-D INVASION PSEUDOPODIA MEMBRANE |
title_short |
HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES |
title_full |
HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES |
title_fullStr |
HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES |
title_full_unstemmed |
HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES |
title_sort |
HELA-CELLS EXTEND AND INTERNALIZE PSEUDOPODIA DURING ACTIVE INVASION BY TRYPANOSOMA-CRUZI TRYPOMASTIGOTES |
author |
Schenkman, Sergio [UNIFESP] |
author_facet |
Schenkman, Sergio [UNIFESP] Mortara, R. A. |
author_role |
author |
author2 |
Mortara, R. A. |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) |
dc.contributor.author.fl_str_mv |
Schenkman, Sergio [UNIFESP] Mortara, R. A. |
dc.subject.por.fl_str_mv |
TRYPANOSOMA-CRUZI ACTIN MICROFILAMENTS HELA CELLS CYTOCHALASIN-D INVASION PSEUDOPODIA MEMBRANE |
topic |
TRYPANOSOMA-CRUZI ACTIN MICROFILAMENTS HELA CELLS CYTOCHALASIN-D INVASION PSEUDOPODIA MEMBRANE |
description |
We show here that HeLa cell microfilaments can be stained by phatloidin at the sites of invasion of Trypanosoma cruzi trypomastigotes, one of the infective stages of this protozoan parasite. Concurrently, a projection of the HeLa cell plasmalemma encircles invading parasites. This plasmalemma projection is further internalized and entire membrane protrusions containing parasites are found within cytoplasmic vacuoles of the host cell. Neither the microfilament staining around invading parasites nor the plasma-lemma extension is inhibited by cytochalasin D, a drug that is unable to prevent trypomastigote entry into HeLa cells. The internalization of the membrane expansion, however, is blocked by the drug. These novel observations indicate that although the driving force for T. cruzi penetration comes from the parasite, the cortical target cytoskeleton of the target cell is concomitantly modified. The molecular characterization of this phenomenon may provide a new insight into the understanding of the mechanisms involved in the active penetration of T. cruzi into mammalian cells. |
publishDate |
1992 |
dc.date.none.fl_str_mv |
1992-04-01 2018-06-15T17:53:09Z 2018-06-15T17:53:09Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://jcs.biologists.org/content/101/4/895 Journal Of Cell Science. Cambridge: Company Of Biologists Ltd, v. 101, p. 895-905, 1992. 0021-9533 http://repositorio.unifesp.br/handle/11600/44231 WOS:A1992HR16000016 |
url |
http://jcs.biologists.org/content/101/4/895 http://repositorio.unifesp.br/handle/11600/44231 |
identifier_str_mv |
Journal Of Cell Science. Cambridge: Company Of Biologists Ltd, v. 101, p. 895-905, 1992. 0021-9533 WOS:A1992HR16000016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal Of Cell Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
895-905 |
dc.publisher.none.fl_str_mv |
Company Of Biologists Ltd |
publisher.none.fl_str_mv |
Company Of Biologists Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
instname_str |
Universidade Federal de São Paulo (UNIFESP) |
instacron_str |
UNIFESP |
institution |
UNIFESP |
reponame_str |
Repositório Institucional da UNIFESP |
collection |
Repositório Institucional da UNIFESP |
repository.name.fl_str_mv |
Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
repository.mail.fl_str_mv |
biblioteca.csp@unifesp.br |
_version_ |
1814268463081848832 |