Optimization of xylanase production by filamentous fungi in solid state fermentation and scale-up to horizontal tube bioreactor
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/31641 |
Resumo: | Five microorganisms, namely Aspergillus niger CECT 2700, A. niger CECT 2915, A. niger CECT 2088, Aspergillus terreus CECT 2808, and Rhizopus stolonifer CECT 2344, were grown on corncob to produce cell wall polysaccharide-degrading enzymes, mainly xylanases, by solid-state fermentation (SSF). A. niger CECT 2700 produced the highest amount of xylanases of 504±7 U/g dry corncob (dcc) after 3 days of fermentation. The optimization of the culture broth (5.0 g/L NaNO3, 1.3 g/L (NH4)2SO4, 4.5 g/L KH2PO4, and 3 g/L yeast extract) and operational conditions (5 g of bed loading, using an initial substrate to moistening medium of 1:3.6 (w/v)) allowed increasing the predicted maximal xylanase activity up to 2,452.7 U/g dcc. However, different pretreatments of materials, including destarching, autoclaving, microwave, and alkaline treatments, were detrimental. Finally, the process was successfully established in a laboratory-scale horizontal tube biore- actor, achieving the highest xylanase activity (2,926 U/g dcc) at a flow rate of 0.2 L/min. The result showed an overall 5.8-fold increase in xylanase activity after optimization of culture media, operational conditions, and scale-up. |
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Optimization of xylanase production by filamentous fungi in solid state fermentation and scale-up to horizontal tube bioreactorCorncobXylanasesSolid-state fermentationAspergillus nigerScience & TechnologyFive microorganisms, namely Aspergillus niger CECT 2700, A. niger CECT 2915, A. niger CECT 2088, Aspergillus terreus CECT 2808, and Rhizopus stolonifer CECT 2344, were grown on corncob to produce cell wall polysaccharide-degrading enzymes, mainly xylanases, by solid-state fermentation (SSF). A. niger CECT 2700 produced the highest amount of xylanases of 504±7 U/g dry corncob (dcc) after 3 days of fermentation. The optimization of the culture broth (5.0 g/L NaNO3, 1.3 g/L (NH4)2SO4, 4.5 g/L KH2PO4, and 3 g/L yeast extract) and operational conditions (5 g of bed loading, using an initial substrate to moistening medium of 1:3.6 (w/v)) allowed increasing the predicted maximal xylanase activity up to 2,452.7 U/g dcc. However, different pretreatments of materials, including destarching, autoclaving, microwave, and alkaline treatments, were detrimental. Finally, the process was successfully established in a laboratory-scale horizontal tube biore- actor, achieving the highest xylanase activity (2,926 U/g dcc) at a flow rate of 0.2 L/min. The result showed an overall 5.8-fold increase in xylanase activity after optimization of culture media, operational conditions, and scale-up.We are grateful to the Spanish Ministry of Science and Innovation for the financial support of this work (project CTQ2011-28967), which has partial financial support from the FEDER funds of the European Union; to the Leonardo da Vinci Programme for founding the stay of Felisbela Oliveira in Vigo University; to MAEC-AECID (Spanish Government) for the financial support for Perez-Bibbins, B. and to Spanish Ministry of Education, Culture and Sports for Perez-Rodriguez's FPU; and to Solla E. and Mendez J. (CACTI-University of Vigo) for their excellent technical assistance in microscopy.SpringerUniversidade do MinhoPérez-Rodríguez, N.Oliveira, FelisbelaPérez-Bibbins, B.Belo, IsabelTorrado Agrasar, A.Domínguez, José Manuel20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31641engPérez-Rodríguez, N.; Oliveira, Felisbela; Pérez-Bibbins, B.; Belo, Isabel; Torrado Agrasar, A.; Domínguez, J. M. , Optimization of xylanase production by filamentous fungi in solid state fermentation and scale-up to horizontal tube bioreactor. Applied Biochemistry and Biotechnology, 173, 803-825, 20140273-22890273-228910.1007/s12010-014-0895-124728763http://link.springer.com/journal/12010info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:42:45Zoai:repositorium.sdum.uminho.pt:1822/31641Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:40:04.666582Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Optimization of xylanase production by filamentous fungi in solid state fermentation and scale-up to horizontal tube bioreactor |
title |
Optimization of xylanase production by filamentous fungi in solid state fermentation and scale-up to horizontal tube bioreactor |
spellingShingle |
Optimization of xylanase production by filamentous fungi in solid state fermentation and scale-up to horizontal tube bioreactor Pérez-Rodríguez, N. Corncob Xylanases Solid-state fermentation Aspergillus niger Science & Technology |
title_short |
Optimization of xylanase production by filamentous fungi in solid state fermentation and scale-up to horizontal tube bioreactor |
title_full |
Optimization of xylanase production by filamentous fungi in solid state fermentation and scale-up to horizontal tube bioreactor |
title_fullStr |
Optimization of xylanase production by filamentous fungi in solid state fermentation and scale-up to horizontal tube bioreactor |
title_full_unstemmed |
Optimization of xylanase production by filamentous fungi in solid state fermentation and scale-up to horizontal tube bioreactor |
title_sort |
Optimization of xylanase production by filamentous fungi in solid state fermentation and scale-up to horizontal tube bioreactor |
author |
Pérez-Rodríguez, N. |
author_facet |
Pérez-Rodríguez, N. Oliveira, Felisbela Pérez-Bibbins, B. Belo, Isabel Torrado Agrasar, A. Domínguez, José Manuel |
author_role |
author |
author2 |
Oliveira, Felisbela Pérez-Bibbins, B. Belo, Isabel Torrado Agrasar, A. Domínguez, José Manuel |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pérez-Rodríguez, N. Oliveira, Felisbela Pérez-Bibbins, B. Belo, Isabel Torrado Agrasar, A. Domínguez, José Manuel |
dc.subject.por.fl_str_mv |
Corncob Xylanases Solid-state fermentation Aspergillus niger Science & Technology |
topic |
Corncob Xylanases Solid-state fermentation Aspergillus niger Science & Technology |
description |
Five microorganisms, namely Aspergillus niger CECT 2700, A. niger CECT 2915, A. niger CECT 2088, Aspergillus terreus CECT 2808, and Rhizopus stolonifer CECT 2344, were grown on corncob to produce cell wall polysaccharide-degrading enzymes, mainly xylanases, by solid-state fermentation (SSF). A. niger CECT 2700 produced the highest amount of xylanases of 504±7 U/g dry corncob (dcc) after 3 days of fermentation. The optimization of the culture broth (5.0 g/L NaNO3, 1.3 g/L (NH4)2SO4, 4.5 g/L KH2PO4, and 3 g/L yeast extract) and operational conditions (5 g of bed loading, using an initial substrate to moistening medium of 1:3.6 (w/v)) allowed increasing the predicted maximal xylanase activity up to 2,452.7 U/g dcc. However, different pretreatments of materials, including destarching, autoclaving, microwave, and alkaline treatments, were detrimental. Finally, the process was successfully established in a laboratory-scale horizontal tube biore- actor, achieving the highest xylanase activity (2,926 U/g dcc) at a flow rate of 0.2 L/min. The result showed an overall 5.8-fold increase in xylanase activity after optimization of culture media, operational conditions, and scale-up. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31641 |
url |
http://hdl.handle.net/1822/31641 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pérez-Rodríguez, N.; Oliveira, Felisbela; Pérez-Bibbins, B.; Belo, Isabel; Torrado Agrasar, A.; Domínguez, J. M. , Optimization of xylanase production by filamentous fungi in solid state fermentation and scale-up to horizontal tube bioreactor. Applied Biochemistry and Biotechnology, 173, 803-825, 2014 0273-2289 0273-2289 10.1007/s12010-014-0895-1 24728763 http://link.springer.com/journal/12010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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