Quality control of the micro-ELISA technic applied to the diagnosis of human and canine visceral leishmaniasis.
Autor(a) principal: | |
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Data de Publicação: | 1995 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/2762 |
Resumo: | Visceral leishmaniasis (VL) or kala-azar is, in Portugal, a zoonosis with the dog as reservoir. A quality control of the technique of micro-ELISA was carried out, using as reference the technique of IFI, the most commonly used for the diagnosis of this protozoosis, both for human and canine sera. Three different methods were used to estimate the cut-off point: X + 2sd (average for negative sera plus two standard deviation), P/N and J index. As quality parameteres were used sensitivity, specificity, efficacy and positive predictive value. The cut-off point for human sera was established at 0.100 A, with 100% sensitivity, 90.5% specificity, 95.3% efficacy and 91.4% positive predictive value, and for canine sera in 0.200 A, with 80.0% sensitivity, 94.3% specificity, 87.7% efficacy and 96.6% positive predictive value. Reproducibility was not fully satisfactory and two different ways of improving it are proposed: P/N and a correction factor. A statistically significant correlation was observed between micro-ELISA's absorbances and IFI titres regarding human sera, though it was not possible to do the same for dog sera. |
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Quality control of the micro-ELISA technic applied to the diagnosis of human and canine visceral leishmaniasis.Controlo de qualidade da technica de micro-ELISA aplicada ao diagnostico da leishmaniose visceral humana e canina.Visceral leishmaniasis (VL) or kala-azar is, in Portugal, a zoonosis with the dog as reservoir. A quality control of the technique of micro-ELISA was carried out, using as reference the technique of IFI, the most commonly used for the diagnosis of this protozoosis, both for human and canine sera. Three different methods were used to estimate the cut-off point: X + 2sd (average for negative sera plus two standard deviation), P/N and J index. As quality parameteres were used sensitivity, specificity, efficacy and positive predictive value. The cut-off point for human sera was established at 0.100 A, with 100% sensitivity, 90.5% specificity, 95.3% efficacy and 91.4% positive predictive value, and for canine sera in 0.200 A, with 80.0% sensitivity, 94.3% specificity, 87.7% efficacy and 96.6% positive predictive value. Reproducibility was not fully satisfactory and two different ways of improving it are proposed: P/N and a correction factor. A statistically significant correlation was observed between micro-ELISA's absorbances and IFI titres regarding human sera, though it was not possible to do the same for dog sera.Visceral leishmaniasis (VL) or kala-azar is, in Portugal, a zoonosis with the dog as reservoir. A quality control of the technique of micro-ELISA was carried out, using as reference the technique of IFI, the most commonly used for the diagnosis of this protozoosis, both for human and canine sera. Three different methods were used to estimate the cut-off point: X + 2sd (average for negative sera plus two standard deviation), P/N and J index. As quality parameteres were used sensitivity, specificity, efficacy and positive predictive value. The cut-off point for human sera was established at 0.100 A, with 100% sensitivity, 90.5% specificity, 95.3% efficacy and 91.4% positive predictive value, and for canine sera in 0.200 A, with 80.0% sensitivity, 94.3% specificity, 87.7% efficacy and 96.6% positive predictive value. Reproducibility was not fully satisfactory and two different ways of improving it are proposed: P/N and a correction factor. A statistically significant correlation was observed between micro-ELISA's absorbances and IFI titres regarding human sera, though it was not possible to do the same for dog sera.Ordem dos Médicos1995-11-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/2762oai:ojs.www.actamedicaportuguesa.com:article/2762Acta Médica Portuguesa; Vol. 8 No. 11 (1995): Novembro; 607-11Acta Médica Portuguesa; Vol. 8 N.º 11 (1995): Novembro; 607-111646-07580870-399Xreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPporhttps://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/2762https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/2762/2152Mauricio, ICampino, LAbranches, Pinfo:eu-repo/semantics/openAccess2022-12-20T11:01:09Zoai:ojs.www.actamedicaportuguesa.com:article/2762Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:17:57.452957Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Quality control of the micro-ELISA technic applied to the diagnosis of human and canine visceral leishmaniasis. Controlo de qualidade da technica de micro-ELISA aplicada ao diagnostico da leishmaniose visceral humana e canina. |
title |
Quality control of the micro-ELISA technic applied to the diagnosis of human and canine visceral leishmaniasis. |
spellingShingle |
Quality control of the micro-ELISA technic applied to the diagnosis of human and canine visceral leishmaniasis. Mauricio, I |
title_short |
Quality control of the micro-ELISA technic applied to the diagnosis of human and canine visceral leishmaniasis. |
title_full |
Quality control of the micro-ELISA technic applied to the diagnosis of human and canine visceral leishmaniasis. |
title_fullStr |
Quality control of the micro-ELISA technic applied to the diagnosis of human and canine visceral leishmaniasis. |
title_full_unstemmed |
Quality control of the micro-ELISA technic applied to the diagnosis of human and canine visceral leishmaniasis. |
title_sort |
Quality control of the micro-ELISA technic applied to the diagnosis of human and canine visceral leishmaniasis. |
author |
Mauricio, I |
author_facet |
Mauricio, I Campino, L Abranches, P |
author_role |
author |
author2 |
Campino, L Abranches, P |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Mauricio, I Campino, L Abranches, P |
description |
Visceral leishmaniasis (VL) or kala-azar is, in Portugal, a zoonosis with the dog as reservoir. A quality control of the technique of micro-ELISA was carried out, using as reference the technique of IFI, the most commonly used for the diagnosis of this protozoosis, both for human and canine sera. Three different methods were used to estimate the cut-off point: X + 2sd (average for negative sera plus two standard deviation), P/N and J index. As quality parameteres were used sensitivity, specificity, efficacy and positive predictive value. The cut-off point for human sera was established at 0.100 A, with 100% sensitivity, 90.5% specificity, 95.3% efficacy and 91.4% positive predictive value, and for canine sera in 0.200 A, with 80.0% sensitivity, 94.3% specificity, 87.7% efficacy and 96.6% positive predictive value. Reproducibility was not fully satisfactory and two different ways of improving it are proposed: P/N and a correction factor. A statistically significant correlation was observed between micro-ELISA's absorbances and IFI titres regarding human sera, though it was not possible to do the same for dog sera. |
publishDate |
1995 |
dc.date.none.fl_str_mv |
1995-11-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/2762 oai:ojs.www.actamedicaportuguesa.com:article/2762 |
url |
https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/2762 |
identifier_str_mv |
oai:ojs.www.actamedicaportuguesa.com:article/2762 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/2762 https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/2762/2152 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Ordem dos Médicos |
publisher.none.fl_str_mv |
Ordem dos Médicos |
dc.source.none.fl_str_mv |
Acta Médica Portuguesa; Vol. 8 No. 11 (1995): Novembro; 607-11 Acta Médica Portuguesa; Vol. 8 N.º 11 (1995): Novembro; 607-11 1646-0758 0870-399X reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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