Application of direct immunofluorescence technic for the diagnosis of canine visceral leishmaniasis in lymph nodes aspirate

Detalhes bibliográficos
Autor(a) principal: Moreira, Marcio Antônio Batistela
Data de Publicação: 2002
Outros Autores: Luvizotto, Maria Cecília Rui, Nunes, Cáris Marone, Silva, Tereza Cristina Cardoso da, Laurenti, Márcia Dalastra, Corbett, Carlos Eduardo Pereira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/5989
Resumo: Recently, canine visceral leishmaniasis (CVL) was detected in the northwest of São Paulo State - Brazil. The Veterinary Hospital - UNESP - Araçatuba, in 2.000, carried out 60 cytopathological test of suspected cases of leishmaniasis using the fine-needle aspiration (FNA). The smears of the FNA popliteal lymph nodes were stained by the Romanowsky stain (Diff-Quik®) and observed using a light microscope. The positive cases showed typical amastigotes forms of Leishmania either free or in macrophage vacuoles. Cytopathological signs of reactivity of the lymph-hystiocitary system with the absence of parasites were also detected. In order to improve the diagnosis of CVL, looking for parasites and antigenic material detection in smears, we standardized the direct immunofluorescence assay (IFD) using mouse anti-Leishmania policlonal antibodies. We compared the IFD assay with the direct search of parasites in smears stained by Romanowsky stain. From the 60 dogs with clinical signs of the disease, the direct exam was positive in 50% (n=30), uncertain in 36,7% (n=22) and negative with lymph node reativity in 13,3% (n=8). When the lymph node smears were submitted to IFD assay we observed positive reaction in 93,3% (n=56) and negative reaction in 6,7% (n=4). Our results showed that IFD assay presented a high sensibility compared to parasite direct search by Romanowsky stain. The IFD assay could be useful method to confirm the uncertain cases of the disease, where amastigotes forms were not clearly identified.
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spelling Application of direct immunofluorescence technic for the diagnosis of canine visceral leishmaniasis in lymph nodes aspirateAplicação da técnica de imunofluorescência direta para o diagnóstico da leishmaniose visceral canina em aspirado de linfonodoImunofluorescênciaLeishmaniose visceralLinfonodos de animalCãesImmunofluorescenceVisceral leishmaniasisLymph nodesDogsRecently, canine visceral leishmaniasis (CVL) was detected in the northwest of São Paulo State - Brazil. The Veterinary Hospital - UNESP - Araçatuba, in 2.000, carried out 60 cytopathological test of suspected cases of leishmaniasis using the fine-needle aspiration (FNA). The smears of the FNA popliteal lymph nodes were stained by the Romanowsky stain (Diff-Quik®) and observed using a light microscope. The positive cases showed typical amastigotes forms of Leishmania either free or in macrophage vacuoles. Cytopathological signs of reactivity of the lymph-hystiocitary system with the absence of parasites were also detected. In order to improve the diagnosis of CVL, looking for parasites and antigenic material detection in smears, we standardized the direct immunofluorescence assay (IFD) using mouse anti-Leishmania policlonal antibodies. We compared the IFD assay with the direct search of parasites in smears stained by Romanowsky stain. From the 60 dogs with clinical signs of the disease, the direct exam was positive in 50% (n=30), uncertain in 36,7% (n=22) and negative with lymph node reativity in 13,3% (n=8). When the lymph node smears were submitted to IFD assay we observed positive reaction in 93,3% (n=56) and negative reaction in 6,7% (n=4). Our results showed that IFD assay presented a high sensibility compared to parasite direct search by Romanowsky stain. The IFD assay could be useful method to confirm the uncertain cases of the disease, where amastigotes forms were not clearly identified.Recentemente, foco de leishmaniose visceral canina (CVL) foi descrito na região noroeste do Estado de São Paulo - Brasil. O Hospital Veterinário - UNESP - Araçatuba, no ano de 2.000, desenvolveu 60 testes citopatológicos de casos suspeitos de leishmaniose usando aspirado por agulha fina (FNA). Os esfregaços de linfonodo foram corados pelo método de Romanowsky (Diff-Quik®) e observados em microscopia de luz. Os casos positivos mostraram formas amastigotas típicas de Leishmania livres ou em vacúolos de macrófagos. Sinais citopatológicos de reatividade do sistema linfo-histiocitário com ausência de parasitos foram também observados. Com o objetivo de implementar o diagnóstico da CVL, detectando parasitos e material antigênico nos esfregaços, aplicou-se a reação de imunofluorescência direta (IFD) usando anticorpo policlonal anti-Leishmania produzido em camundongo. Comparamos o método de IFD com a pesquisa direta do parasito em esfregaços corados pelo método de Romanowsky. Dos 60 cães com sinais clínicos da doença, o exame direto foi positivo em 50% (n=30), duvidoso em 36,7% (n=22) e negativo com reatividade do linfonodo em 13,3% (n=8). Quando os linfonodos foram submetidos a reação de IFD observamos reação positiva em 93,3% (n=56) e reação negativa em 6,7% (n=4). Nossos resultados mostraram que a reação de IFD apresentou alta sensibilidade quando comparada a pesquisa direta do parasito pela coloração de Romanowsky. A reação de IFD pode ser um método útil para confirmar os casos duvidosos da doença, onde as formas amastigotas não são identificadas com facilidade.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2002-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/598910.1590/S1413-95962002000200009Brazilian Journal of Veterinary Research and Animal Science; Vol. 39 Núm. 2 (2002); 103-106Brazilian Journal of Veterinary Research and Animal Science; Vol. 39 No. 2 (2002); 103-106Brazilian Journal of Veterinary Research and Animal Science; v. 39 n. 2 (2002); 103-106Brazilian Journal of Veterinary Research and Animal Science; V. 39 N. 2 (2002); 103-1061678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/5989/7520Moreira, Marcio Antônio BatistelaLuvizotto, Maria Cecília RuiNunes, Cáris MaroneSilva, Tereza Cristina Cardoso daLaurenti, Márcia DalastraCorbett, Carlos Eduardo Pereirainfo:eu-repo/semantics/openAccess2020-06-23T04:36:20Zoai:revistas.usp.br:article/5989Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:33.392647Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Application of direct immunofluorescence technic for the diagnosis of canine visceral leishmaniasis in lymph nodes aspirate
Aplicação da técnica de imunofluorescência direta para o diagnóstico da leishmaniose visceral canina em aspirado de linfonodo
title Application of direct immunofluorescence technic for the diagnosis of canine visceral leishmaniasis in lymph nodes aspirate
spellingShingle Application of direct immunofluorescence technic for the diagnosis of canine visceral leishmaniasis in lymph nodes aspirate
Moreira, Marcio Antônio Batistela
Imunofluorescência
Leishmaniose visceral
Linfonodos de animal
Cães
Immunofluorescence
Visceral leishmaniasis
Lymph nodes
Dogs
title_short Application of direct immunofluorescence technic for the diagnosis of canine visceral leishmaniasis in lymph nodes aspirate
title_full Application of direct immunofluorescence technic for the diagnosis of canine visceral leishmaniasis in lymph nodes aspirate
title_fullStr Application of direct immunofluorescence technic for the diagnosis of canine visceral leishmaniasis in lymph nodes aspirate
title_full_unstemmed Application of direct immunofluorescence technic for the diagnosis of canine visceral leishmaniasis in lymph nodes aspirate
title_sort Application of direct immunofluorescence technic for the diagnosis of canine visceral leishmaniasis in lymph nodes aspirate
author Moreira, Marcio Antônio Batistela
author_facet Moreira, Marcio Antônio Batistela
Luvizotto, Maria Cecília Rui
Nunes, Cáris Marone
Silva, Tereza Cristina Cardoso da
Laurenti, Márcia Dalastra
Corbett, Carlos Eduardo Pereira
author_role author
author2 Luvizotto, Maria Cecília Rui
Nunes, Cáris Marone
Silva, Tereza Cristina Cardoso da
Laurenti, Márcia Dalastra
Corbett, Carlos Eduardo Pereira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Moreira, Marcio Antônio Batistela
Luvizotto, Maria Cecília Rui
Nunes, Cáris Marone
Silva, Tereza Cristina Cardoso da
Laurenti, Márcia Dalastra
Corbett, Carlos Eduardo Pereira
dc.subject.por.fl_str_mv Imunofluorescência
Leishmaniose visceral
Linfonodos de animal
Cães
Immunofluorescence
Visceral leishmaniasis
Lymph nodes
Dogs
topic Imunofluorescência
Leishmaniose visceral
Linfonodos de animal
Cães
Immunofluorescence
Visceral leishmaniasis
Lymph nodes
Dogs
description Recently, canine visceral leishmaniasis (CVL) was detected in the northwest of São Paulo State - Brazil. The Veterinary Hospital - UNESP - Araçatuba, in 2.000, carried out 60 cytopathological test of suspected cases of leishmaniasis using the fine-needle aspiration (FNA). The smears of the FNA popliteal lymph nodes were stained by the Romanowsky stain (Diff-Quik®) and observed using a light microscope. The positive cases showed typical amastigotes forms of Leishmania either free or in macrophage vacuoles. Cytopathological signs of reactivity of the lymph-hystiocitary system with the absence of parasites were also detected. In order to improve the diagnosis of CVL, looking for parasites and antigenic material detection in smears, we standardized the direct immunofluorescence assay (IFD) using mouse anti-Leishmania policlonal antibodies. We compared the IFD assay with the direct search of parasites in smears stained by Romanowsky stain. From the 60 dogs with clinical signs of the disease, the direct exam was positive in 50% (n=30), uncertain in 36,7% (n=22) and negative with lymph node reativity in 13,3% (n=8). When the lymph node smears were submitted to IFD assay we observed positive reaction in 93,3% (n=56) and negative reaction in 6,7% (n=4). Our results showed that IFD assay presented a high sensibility compared to parasite direct search by Romanowsky stain. The IFD assay could be useful method to confirm the uncertain cases of the disease, where amastigotes forms were not clearly identified.
publishDate 2002
dc.date.none.fl_str_mv 2002-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/5989
10.1590/S1413-95962002000200009
url https://www.revistas.usp.br/bjvras/article/view/5989
identifier_str_mv 10.1590/S1413-95962002000200009
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/5989/7520
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 39 Núm. 2 (2002); 103-106
Brazilian Journal of Veterinary Research and Animal Science; Vol. 39 No. 2 (2002); 103-106
Brazilian Journal of Veterinary Research and Animal Science; v. 39 n. 2 (2002); 103-106
Brazilian Journal of Veterinary Research and Animal Science; V. 39 N. 2 (2002); 103-106
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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