Distinct microbial populations are tightly linked to the profile of dissolved iron in the methanic sediments of the Helgoland mud area, North Sea
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/109151 https://doi.org/10.3389/fmicb.2015.00365 |
Resumo: | Iron reduction in subseafloor sulfate-depleted and methane-rich marine sediments is currently a subject of interest in subsurface geomicrobiology. While iron reduction and microorganisms involved have been well studied in marine surface sediments, little is known about microorganisms responsible for iron reduction in deep methanic sediments. Here, we used quantitative PCR-based 16S rRNA gene copy numbers and pyrosequencing-based relative abundances of bacteria and archaea to investigate covariance between distinct microbial populations and specific geochemical profiles in the top 5 m of sediment cores from the Helgoland mud area, North Sea. We found that gene copy numbers of bacteria and archaea were specifically higher around the peak of dissolved iron in the methanic zone (250-350 cm). The higher copy numbers at these depths were also reflected by the relative sequence abundances of members of the candidate division JS1, methanogenic and Methanohalobium/ANME-3 related archaea. The distribution of these populations was strongly correlated to the profile of pore-water Fe(2+) while that of Desulfobacteraceae corresponded to the pore-water sulfate profile. Furthermore, specific JS1 populations also strongly co-varied with the distribution of Methanosaetaceae in the methanic zone. Our data suggest that the interplay among JS1 bacteria, methanogenic archaea and Methanohalobium/ANME-3-related archaea may be important for iron reduction and methane cycling in deep methanic sediments of the Helgoland mud area and perhaps in other methane-rich depositional environments. |
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Distinct microbial populations are tightly linked to the profile of dissolved iron in the methanic sediments of the Helgoland mud area, North Seacandidate division JS1iron reductionmethanogensANMEsubsurface sedimentsNorthSeaSMTanaerobic oxidation of methaneIron reduction in subseafloor sulfate-depleted and methane-rich marine sediments is currently a subject of interest in subsurface geomicrobiology. While iron reduction and microorganisms involved have been well studied in marine surface sediments, little is known about microorganisms responsible for iron reduction in deep methanic sediments. Here, we used quantitative PCR-based 16S rRNA gene copy numbers and pyrosequencing-based relative abundances of bacteria and archaea to investigate covariance between distinct microbial populations and specific geochemical profiles in the top 5 m of sediment cores from the Helgoland mud area, North Sea. We found that gene copy numbers of bacteria and archaea were specifically higher around the peak of dissolved iron in the methanic zone (250-350 cm). The higher copy numbers at these depths were also reflected by the relative sequence abundances of members of the candidate division JS1, methanogenic and Methanohalobium/ANME-3 related archaea. The distribution of these populations was strongly correlated to the profile of pore-water Fe(2+) while that of Desulfobacteraceae corresponded to the pore-water sulfate profile. Furthermore, specific JS1 populations also strongly co-varied with the distribution of Methanosaetaceae in the methanic zone. Our data suggest that the interplay among JS1 bacteria, methanogenic archaea and Methanohalobium/ANME-3-related archaea may be important for iron reduction and methane cycling in deep methanic sediments of the Helgoland mud area and perhaps in other methane-rich depositional environments.ThisstudywassupportedbytheResearchCenter/Clusterof Excellence‘TheOceanintheEarthSystem’(MARUM)funded bytheDeutscheForschungsgemeinschaft(DFG)andbythe UniversityofBremen.Theauthorsthankthecaptain,crew,and scientistsofR/VHEINCKEexpeditionsHE376,HE406,and HE421. WealsothankthecaptainandcrewofRVUTHÖRN fortheirhelpduringUT-2012sampling.Inaddition,wethank BenjaminLöfflerandRoiMartinezfortheirhelpwithon- boardgeochemicalmeasurementsandcreationofthemapof theHelgolandmudarea,respectively.Weacknowledgeaddi- tionalfundingbytheHelmholtzAssociation(AlfredWegener InstituteHelmholtzCentreforPolarandMarineResearch) in theframeworkoftheresearchprogramsPACESIand PACESII.BCgratefullyacknowledgestheFCT/COMPETEPEst- C/FIS/UI0036/2011project.Frontiers Media S.A.2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/109151http://hdl.handle.net/10316/109151https://doi.org/10.3389/fmicb.2015.00365eng1664-302X25983723Oni, OluwatobiMiyatake, TetsuroKasten, SabineRichter-Heitmann, TimFischer, DavidWagenknecht, LauraKulkarni, AjinkyaBlumers, MathiasShylin, Sergii I.Ksenofontov, VadimCosta, Benilde F. O.Klingelhöfer, GöstarFriedrich, Michael W.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-09-29T08:27:49Zoai:estudogeral.uc.pt:10316/109151Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:25:20.975010Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Distinct microbial populations are tightly linked to the profile of dissolved iron in the methanic sediments of the Helgoland mud area, North Sea |
title |
Distinct microbial populations are tightly linked to the profile of dissolved iron in the methanic sediments of the Helgoland mud area, North Sea |
spellingShingle |
Distinct microbial populations are tightly linked to the profile of dissolved iron in the methanic sediments of the Helgoland mud area, North Sea Oni, Oluwatobi candidate division JS1 iron reduction methanogens ANME subsurface sediments NorthSea SMT anaerobic oxidation of methane |
title_short |
Distinct microbial populations are tightly linked to the profile of dissolved iron in the methanic sediments of the Helgoland mud area, North Sea |
title_full |
Distinct microbial populations are tightly linked to the profile of dissolved iron in the methanic sediments of the Helgoland mud area, North Sea |
title_fullStr |
Distinct microbial populations are tightly linked to the profile of dissolved iron in the methanic sediments of the Helgoland mud area, North Sea |
title_full_unstemmed |
Distinct microbial populations are tightly linked to the profile of dissolved iron in the methanic sediments of the Helgoland mud area, North Sea |
title_sort |
Distinct microbial populations are tightly linked to the profile of dissolved iron in the methanic sediments of the Helgoland mud area, North Sea |
author |
Oni, Oluwatobi |
author_facet |
Oni, Oluwatobi Miyatake, Tetsuro Kasten, Sabine Richter-Heitmann, Tim Fischer, David Wagenknecht, Laura Kulkarni, Ajinkya Blumers, Mathias Shylin, Sergii I. Ksenofontov, Vadim Costa, Benilde F. O. Klingelhöfer, Göstar Friedrich, Michael W. |
author_role |
author |
author2 |
Miyatake, Tetsuro Kasten, Sabine Richter-Heitmann, Tim Fischer, David Wagenknecht, Laura Kulkarni, Ajinkya Blumers, Mathias Shylin, Sergii I. Ksenofontov, Vadim Costa, Benilde F. O. Klingelhöfer, Göstar Friedrich, Michael W. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oni, Oluwatobi Miyatake, Tetsuro Kasten, Sabine Richter-Heitmann, Tim Fischer, David Wagenknecht, Laura Kulkarni, Ajinkya Blumers, Mathias Shylin, Sergii I. Ksenofontov, Vadim Costa, Benilde F. O. Klingelhöfer, Göstar Friedrich, Michael W. |
dc.subject.por.fl_str_mv |
candidate division JS1 iron reduction methanogens ANME subsurface sediments NorthSea SMT anaerobic oxidation of methane |
topic |
candidate division JS1 iron reduction methanogens ANME subsurface sediments NorthSea SMT anaerobic oxidation of methane |
description |
Iron reduction in subseafloor sulfate-depleted and methane-rich marine sediments is currently a subject of interest in subsurface geomicrobiology. While iron reduction and microorganisms involved have been well studied in marine surface sediments, little is known about microorganisms responsible for iron reduction in deep methanic sediments. Here, we used quantitative PCR-based 16S rRNA gene copy numbers and pyrosequencing-based relative abundances of bacteria and archaea to investigate covariance between distinct microbial populations and specific geochemical profiles in the top 5 m of sediment cores from the Helgoland mud area, North Sea. We found that gene copy numbers of bacteria and archaea were specifically higher around the peak of dissolved iron in the methanic zone (250-350 cm). The higher copy numbers at these depths were also reflected by the relative sequence abundances of members of the candidate division JS1, methanogenic and Methanohalobium/ANME-3 related archaea. The distribution of these populations was strongly correlated to the profile of pore-water Fe(2+) while that of Desulfobacteraceae corresponded to the pore-water sulfate profile. Furthermore, specific JS1 populations also strongly co-varied with the distribution of Methanosaetaceae in the methanic zone. Our data suggest that the interplay among JS1 bacteria, methanogenic archaea and Methanohalobium/ANME-3-related archaea may be important for iron reduction and methane cycling in deep methanic sediments of the Helgoland mud area and perhaps in other methane-rich depositional environments. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/109151 http://hdl.handle.net/10316/109151 https://doi.org/10.3389/fmicb.2015.00365 |
url |
http://hdl.handle.net/10316/109151 https://doi.org/10.3389/fmicb.2015.00365 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1664-302X 25983723 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Frontiers Media S.A. |
publisher.none.fl_str_mv |
Frontiers Media S.A. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799134136434163712 |