The effect of the shape of gold core-mesoporous silica shell nanoparticles on the cellular behavior and tumor spheroid penetration
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/4689 |
Resumo: | Size, surface charge, and shape have a huge influence on the behavior, cellular uptake, and cytotoxic profile of nanoparticles. Herein, gold core and silica shell based nanoparticles (Au–MSSs) with spherical or rod-like shape were produced, in order to disclose the effect of the shape of nanomaterials on the cellular uptake, reactive oxygen species (ROS) production, 3D tumor spheroid penetration and cytotoxicity towards cancer cells. The Au–MSS spheres induced greater reduction of the ROS content of cancer cells and also displayed a more homogeneous distribution and penetration in 3D tumor spheroids. However, the Au–MSS rods show enhanced cellular migration and uptake in 2D cell cultures, which results in a higher drug delivering capacity. Furthermore, the Au–MSS rods displayed an enhanced cellular cytotoxicity upon laser irradiation (808 nm, 1.7 W cm−2, 10 min), where less than 10% of cells remained viable. In addition, both Au–MSSs have the potential to be used as imaging agents, which further expands their applicability as theranostic agents in the biomedical area. In summary, the obtained results show that the shape of Au–MSSs is crucial for their biological performance, which will have a great influence on their therapeutic outcome. Therefore, our findings may provide useful information for the development and design of new drug delivery systems towards a more effective therapy. |
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The effect of the shape of gold core-mesoporous silica shell nanoparticles on the cellular behavior and tumor spheroid penetrationNanoparticlesGoldMesoporous silicaSpheroidSize, surface charge, and shape have a huge influence on the behavior, cellular uptake, and cytotoxic profile of nanoparticles. Herein, gold core and silica shell based nanoparticles (Au–MSSs) with spherical or rod-like shape were produced, in order to disclose the effect of the shape of nanomaterials on the cellular uptake, reactive oxygen species (ROS) production, 3D tumor spheroid penetration and cytotoxicity towards cancer cells. The Au–MSS spheres induced greater reduction of the ROS content of cancer cells and also displayed a more homogeneous distribution and penetration in 3D tumor spheroids. However, the Au–MSS rods show enhanced cellular migration and uptake in 2D cell cultures, which results in a higher drug delivering capacity. Furthermore, the Au–MSS rods displayed an enhanced cellular cytotoxicity upon laser irradiation (808 nm, 1.7 W cm−2, 10 min), where less than 10% of cells remained viable. In addition, both Au–MSSs have the potential to be used as imaging agents, which further expands their applicability as theranostic agents in the biomedical area. In summary, the obtained results show that the shape of Au–MSSs is crucial for their biological performance, which will have a great influence on their therapeutic outcome. Therefore, our findings may provide useful information for the development and design of new drug delivery systems towards a more effective therapy.Royal Society of ChemistryuBibliorumDias, Diana RodriguesMoreira, AndréCorreia, Ilídio Joaquim Sobreira2018-03-21T15:30:21Z2016-11-012016-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4689eng10.1039/C6TB02668Kmetadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-15T09:41:57Zoai:ubibliorum.ubi.pt:10400.6/4689Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:45:44.558911Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The effect of the shape of gold core-mesoporous silica shell nanoparticles on the cellular behavior and tumor spheroid penetration |
title |
The effect of the shape of gold core-mesoporous silica shell nanoparticles on the cellular behavior and tumor spheroid penetration |
spellingShingle |
The effect of the shape of gold core-mesoporous silica shell nanoparticles on the cellular behavior and tumor spheroid penetration Dias, Diana Rodrigues Nanoparticles Gold Mesoporous silica Spheroid |
title_short |
The effect of the shape of gold core-mesoporous silica shell nanoparticles on the cellular behavior and tumor spheroid penetration |
title_full |
The effect of the shape of gold core-mesoporous silica shell nanoparticles on the cellular behavior and tumor spheroid penetration |
title_fullStr |
The effect of the shape of gold core-mesoporous silica shell nanoparticles on the cellular behavior and tumor spheroid penetration |
title_full_unstemmed |
The effect of the shape of gold core-mesoporous silica shell nanoparticles on the cellular behavior and tumor spheroid penetration |
title_sort |
The effect of the shape of gold core-mesoporous silica shell nanoparticles on the cellular behavior and tumor spheroid penetration |
author |
Dias, Diana Rodrigues |
author_facet |
Dias, Diana Rodrigues Moreira, André Correia, Ilídio Joaquim Sobreira |
author_role |
author |
author2 |
Moreira, André Correia, Ilídio Joaquim Sobreira |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
uBibliorum |
dc.contributor.author.fl_str_mv |
Dias, Diana Rodrigues Moreira, André Correia, Ilídio Joaquim Sobreira |
dc.subject.por.fl_str_mv |
Nanoparticles Gold Mesoporous silica Spheroid |
topic |
Nanoparticles Gold Mesoporous silica Spheroid |
description |
Size, surface charge, and shape have a huge influence on the behavior, cellular uptake, and cytotoxic profile of nanoparticles. Herein, gold core and silica shell based nanoparticles (Au–MSSs) with spherical or rod-like shape were produced, in order to disclose the effect of the shape of nanomaterials on the cellular uptake, reactive oxygen species (ROS) production, 3D tumor spheroid penetration and cytotoxicity towards cancer cells. The Au–MSS spheres induced greater reduction of the ROS content of cancer cells and also displayed a more homogeneous distribution and penetration in 3D tumor spheroids. However, the Au–MSS rods show enhanced cellular migration and uptake in 2D cell cultures, which results in a higher drug delivering capacity. Furthermore, the Au–MSS rods displayed an enhanced cellular cytotoxicity upon laser irradiation (808 nm, 1.7 W cm−2, 10 min), where less than 10% of cells remained viable. In addition, both Au–MSSs have the potential to be used as imaging agents, which further expands their applicability as theranostic agents in the biomedical area. In summary, the obtained results show that the shape of Au–MSSs is crucial for their biological performance, which will have a great influence on their therapeutic outcome. Therefore, our findings may provide useful information for the development and design of new drug delivery systems towards a more effective therapy. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 2016-11-01T00:00:00Z 2018-03-21T15:30:21Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/4689 |
url |
http://hdl.handle.net/10400.6/4689 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1039/C6TB02668K |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136354524725248 |