A novel hanging spherical drop system for the generation of cellular spheroids and high throughput combinatorial drug screening
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/40673 |
Resumo: | We propose a novel hanging spherical drop system for anchoring arrays of droplets of cell suspension based on the use of biomimetic superhydrophobic flat substrates, with controlled positional adhesion and minimum contact with a solid substrate. By facing down the platform, it was possible to generate independent spheroid bodies in a high throughput manner, in order to mimic in vivo tumour models on the lab-on-chip scale. To validate this system for drug screening purposes, the toxicity of the anti-cancer drug doxorubicin in cell spheroids was tested and compared to cells in 2D culture. The advantages presented by this platform, such as feasibility of the system and the ability to control the size uniformity of the spheroid, emphasize its potential to be used as a new low cost toolbox for high-throughput drug screening and in cell or tissue engineering. |
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A novel hanging spherical drop system for the generation of cellular spheroids and high throughput combinatorial drug screeningHigh-throughput screeningSpheroidsScience & TechnologyWe propose a novel hanging spherical drop system for anchoring arrays of droplets of cell suspension based on the use of biomimetic superhydrophobic flat substrates, with controlled positional adhesion and minimum contact with a solid substrate. By facing down the platform, it was possible to generate independent spheroid bodies in a high throughput manner, in order to mimic in vivo tumour models on the lab-on-chip scale. To validate this system for drug screening purposes, the toxicity of the anti-cancer drug doxorubicin in cell spheroids was tested and compared to cells in 2D culture. The advantages presented by this platform, such as feasibility of the system and the ability to control the size uniformity of the spheroid, emphasize its potential to be used as a new low cost toolbox for high-throughput drug screening and in cell or tissue engineering.The authors thank the precious help of Alessandra Zonari and Rui Domingues for capturing the confocal images. The authors acknowledge the financial support from the FCT-Fundacao para a Ciencia e para a Tecnologia through the Ph.D. grants with the references SFRH/BD/73119/2010, SFRH/BD/69529/2010 and SFRH/BD/71396/2010. We acknowledge the financial support of FEDER through the program Operacional Factores de Competitividade - COMPETE and from FCT - the Fundacao para a Ciencia e a Tecnologia under the project PTDC/CTM-BIO/1814/2012. We also thank the support by the European Research Council grant agreement ERC-2012-ADG 20120216-321266 for the project ComplexiTE.Royal Society of ChemistryUniversidade do MinhoNeto, A. I.Correia, C. R.Oliveira, M. B.Rial-Hermida, M. I.Álvarez-Lorenzo, C.Reis, R. L.Mano, J. F.2015-012015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/40673engNeto, A. I., Correia, C. R., Oliveira, M. B., Rial-Hermida, M. I., Alvarez-Lorenzo, C., Reis, R. L., & Mano, J. F. (2015). A novel hanging spherical drop system for the generation of cellular spheroids and high throughput combinatorial drug screening. Biomaterials Science, 3(4), 581-585. doi: 10.1039/c4bm00411f2047-483010.1039/C4BM00411F26222417http://dx.doi.org/10.1039/c4bm00411finfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:39:40Zoai:repositorium.sdum.uminho.pt:1822/40673Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:36:19.700661Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A novel hanging spherical drop system for the generation of cellular spheroids and high throughput combinatorial drug screening |
title |
A novel hanging spherical drop system for the generation of cellular spheroids and high throughput combinatorial drug screening |
spellingShingle |
A novel hanging spherical drop system for the generation of cellular spheroids and high throughput combinatorial drug screening Neto, A. I. High-throughput screening Spheroids Science & Technology |
title_short |
A novel hanging spherical drop system for the generation of cellular spheroids and high throughput combinatorial drug screening |
title_full |
A novel hanging spherical drop system for the generation of cellular spheroids and high throughput combinatorial drug screening |
title_fullStr |
A novel hanging spherical drop system for the generation of cellular spheroids and high throughput combinatorial drug screening |
title_full_unstemmed |
A novel hanging spherical drop system for the generation of cellular spheroids and high throughput combinatorial drug screening |
title_sort |
A novel hanging spherical drop system for the generation of cellular spheroids and high throughput combinatorial drug screening |
author |
Neto, A. I. |
author_facet |
Neto, A. I. Correia, C. R. Oliveira, M. B. Rial-Hermida, M. I. Álvarez-Lorenzo, C. Reis, R. L. Mano, J. F. |
author_role |
author |
author2 |
Correia, C. R. Oliveira, M. B. Rial-Hermida, M. I. Álvarez-Lorenzo, C. Reis, R. L. Mano, J. F. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Neto, A. I. Correia, C. R. Oliveira, M. B. Rial-Hermida, M. I. Álvarez-Lorenzo, C. Reis, R. L. Mano, J. F. |
dc.subject.por.fl_str_mv |
High-throughput screening Spheroids Science & Technology |
topic |
High-throughput screening Spheroids Science & Technology |
description |
We propose a novel hanging spherical drop system for anchoring arrays of droplets of cell suspension based on the use of biomimetic superhydrophobic flat substrates, with controlled positional adhesion and minimum contact with a solid substrate. By facing down the platform, it was possible to generate independent spheroid bodies in a high throughput manner, in order to mimic in vivo tumour models on the lab-on-chip scale. To validate this system for drug screening purposes, the toxicity of the anti-cancer drug doxorubicin in cell spheroids was tested and compared to cells in 2D culture. The advantages presented by this platform, such as feasibility of the system and the ability to control the size uniformity of the spheroid, emphasize its potential to be used as a new low cost toolbox for high-throughput drug screening and in cell or tissue engineering. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01 2015-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/40673 |
url |
http://hdl.handle.net/1822/40673 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Neto, A. I., Correia, C. R., Oliveira, M. B., Rial-Hermida, M. I., Alvarez-Lorenzo, C., Reis, R. L., & Mano, J. F. (2015). A novel hanging spherical drop system for the generation of cellular spheroids and high throughput combinatorial drug screening. Biomaterials Science, 3(4), 581-585. doi: 10.1039/c4bm00411f 2047-4830 10.1039/C4BM00411F 26222417 http://dx.doi.org/10.1039/c4bm00411f |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132891889795072 |