3D tumor spheroids: an overview on the tools and techniques used for their analysis

Detalhes bibliográficos
Autor(a) principal: Costa, Elisabete C.
Data de Publicação: 2016
Outros Autores: Moreira, André, Diogo, Duarte Miguel de Melo, Gaspar, V. M., Carvalho, Marco António Paulo de, Correia, I.J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.6/4690
Resumo: In comparison with 2D cell culture models, 3D spheroids are able to accurately mimic some features of solid tumors, such as their spatial architecture, physiological responses, secretion of soluble mediators, gene expression patterns and drug resistance mechanisms. These unique characteristics highlight the potential of 3D cellular aggregates to be used as in vitro models for screening new anticancer therapeutics, both at a small and large scale. Nevertheless, few reports have focused on describing the tools and techniques currently available to extract significant biological data from these models. Such information will be fundamental to drug and therapeutic discovery process using 3D cell culture models. The present review provides an overview of the techniques that can be employed to characterize and evaluate the efficacy of anticancer therapeutics in 3D tumor spheroids.
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spelling 3D tumor spheroids: an overview on the tools and techniques used for their analysis3D tumor spheroidsCancer therapyIn vitro modelsMethodologiesTherapeutic screeningIn comparison with 2D cell culture models, 3D spheroids are able to accurately mimic some features of solid tumors, such as their spatial architecture, physiological responses, secretion of soluble mediators, gene expression patterns and drug resistance mechanisms. These unique characteristics highlight the potential of 3D cellular aggregates to be used as in vitro models for screening new anticancer therapeutics, both at a small and large scale. Nevertheless, few reports have focused on describing the tools and techniques currently available to extract significant biological data from these models. Such information will be fundamental to drug and therapeutic discovery process using 3D cell culture models. The present review provides an overview of the techniques that can be employed to characterize and evaluate the efficacy of anticancer therapeutics in 3D tumor spheroids.ElsevieruBibliorumCosta, Elisabete C.Moreira, AndréDiogo, Duarte Miguel de MeloGaspar, V. M.Carvalho, Marco António Paulo deCorreia, I.J.2018-03-21T15:40:51Z2016-11-112016-11-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4690eng10.1016/j.biotechadv.2016.11.002metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-27T12:15:11Zoai:ubibliorum.ubi.pt:10400.6/4690Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-27T12:15:11Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv 3D tumor spheroids: an overview on the tools and techniques used for their analysis
title 3D tumor spheroids: an overview on the tools and techniques used for their analysis
spellingShingle 3D tumor spheroids: an overview on the tools and techniques used for their analysis
Costa, Elisabete C.
3D tumor spheroids
Cancer therapy
In vitro models
Methodologies
Therapeutic screening
title_short 3D tumor spheroids: an overview on the tools and techniques used for their analysis
title_full 3D tumor spheroids: an overview on the tools and techniques used for their analysis
title_fullStr 3D tumor spheroids: an overview on the tools and techniques used for their analysis
title_full_unstemmed 3D tumor spheroids: an overview on the tools and techniques used for their analysis
title_sort 3D tumor spheroids: an overview on the tools and techniques used for their analysis
author Costa, Elisabete C.
author_facet Costa, Elisabete C.
Moreira, André
Diogo, Duarte Miguel de Melo
Gaspar, V. M.
Carvalho, Marco António Paulo de
Correia, I.J.
author_role author
author2 Moreira, André
Diogo, Duarte Miguel de Melo
Gaspar, V. M.
Carvalho, Marco António Paulo de
Correia, I.J.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv uBibliorum
dc.contributor.author.fl_str_mv Costa, Elisabete C.
Moreira, André
Diogo, Duarte Miguel de Melo
Gaspar, V. M.
Carvalho, Marco António Paulo de
Correia, I.J.
dc.subject.por.fl_str_mv 3D tumor spheroids
Cancer therapy
In vitro models
Methodologies
Therapeutic screening
topic 3D tumor spheroids
Cancer therapy
In vitro models
Methodologies
Therapeutic screening
description In comparison with 2D cell culture models, 3D spheroids are able to accurately mimic some features of solid tumors, such as their spatial architecture, physiological responses, secretion of soluble mediators, gene expression patterns and drug resistance mechanisms. These unique characteristics highlight the potential of 3D cellular aggregates to be used as in vitro models for screening new anticancer therapeutics, both at a small and large scale. Nevertheless, few reports have focused on describing the tools and techniques currently available to extract significant biological data from these models. Such information will be fundamental to drug and therapeutic discovery process using 3D cell culture models. The present review provides an overview of the techniques that can be employed to characterize and evaluate the efficacy of anticancer therapeutics in 3D tumor spheroids.
publishDate 2016
dc.date.none.fl_str_mv 2016-11-11
2016-11-11T00:00:00Z
2018-03-21T15:40:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.6/4690
url http://hdl.handle.net/10400.6/4690
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.biotechadv.2016.11.002
dc.rights.driver.fl_str_mv metadata only access
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eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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