3D tumor spheroids: an overview on the tools and techniques used for their analysis
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/4690 |
Resumo: | In comparison with 2D cell culture models, 3D spheroids are able to accurately mimic some features of solid tumors, such as their spatial architecture, physiological responses, secretion of soluble mediators, gene expression patterns and drug resistance mechanisms. These unique characteristics highlight the potential of 3D cellular aggregates to be used as in vitro models for screening new anticancer therapeutics, both at a small and large scale. Nevertheless, few reports have focused on describing the tools and techniques currently available to extract significant biological data from these models. Such information will be fundamental to drug and therapeutic discovery process using 3D cell culture models. The present review provides an overview of the techniques that can be employed to characterize and evaluate the efficacy of anticancer therapeutics in 3D tumor spheroids. |
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3D tumor spheroids: an overview on the tools and techniques used for their analysis3D tumor spheroidsCancer therapyIn vitro modelsMethodologiesTherapeutic screeningIn comparison with 2D cell culture models, 3D spheroids are able to accurately mimic some features of solid tumors, such as their spatial architecture, physiological responses, secretion of soluble mediators, gene expression patterns and drug resistance mechanisms. These unique characteristics highlight the potential of 3D cellular aggregates to be used as in vitro models for screening new anticancer therapeutics, both at a small and large scale. Nevertheless, few reports have focused on describing the tools and techniques currently available to extract significant biological data from these models. Such information will be fundamental to drug and therapeutic discovery process using 3D cell culture models. The present review provides an overview of the techniques that can be employed to characterize and evaluate the efficacy of anticancer therapeutics in 3D tumor spheroids.ElsevieruBibliorumCosta, Elisabete C.Moreira, AndréDiogo, Duarte Miguel de MeloGaspar, V. M.Carvalho, Marco António Paulo deCorreia, I.J.2018-03-21T15:40:51Z2016-11-112016-11-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4690eng10.1016/j.biotechadv.2016.11.002metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-27T12:15:11Zoai:ubibliorum.ubi.pt:10400.6/4690Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-27T12:15:11Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
3D tumor spheroids: an overview on the tools and techniques used for their analysis |
title |
3D tumor spheroids: an overview on the tools and techniques used for their analysis |
spellingShingle |
3D tumor spheroids: an overview on the tools and techniques used for their analysis Costa, Elisabete C. 3D tumor spheroids Cancer therapy In vitro models Methodologies Therapeutic screening |
title_short |
3D tumor spheroids: an overview on the tools and techniques used for their analysis |
title_full |
3D tumor spheroids: an overview on the tools and techniques used for their analysis |
title_fullStr |
3D tumor spheroids: an overview on the tools and techniques used for their analysis |
title_full_unstemmed |
3D tumor spheroids: an overview on the tools and techniques used for their analysis |
title_sort |
3D tumor spheroids: an overview on the tools and techniques used for their analysis |
author |
Costa, Elisabete C. |
author_facet |
Costa, Elisabete C. Moreira, André Diogo, Duarte Miguel de Melo Gaspar, V. M. Carvalho, Marco António Paulo de Correia, I.J. |
author_role |
author |
author2 |
Moreira, André Diogo, Duarte Miguel de Melo Gaspar, V. M. Carvalho, Marco António Paulo de Correia, I.J. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
uBibliorum |
dc.contributor.author.fl_str_mv |
Costa, Elisabete C. Moreira, André Diogo, Duarte Miguel de Melo Gaspar, V. M. Carvalho, Marco António Paulo de Correia, I.J. |
dc.subject.por.fl_str_mv |
3D tumor spheroids Cancer therapy In vitro models Methodologies Therapeutic screening |
topic |
3D tumor spheroids Cancer therapy In vitro models Methodologies Therapeutic screening |
description |
In comparison with 2D cell culture models, 3D spheroids are able to accurately mimic some features of solid tumors, such as their spatial architecture, physiological responses, secretion of soluble mediators, gene expression patterns and drug resistance mechanisms. These unique characteristics highlight the potential of 3D cellular aggregates to be used as in vitro models for screening new anticancer therapeutics, both at a small and large scale. Nevertheless, few reports have focused on describing the tools and techniques currently available to extract significant biological data from these models. Such information will be fundamental to drug and therapeutic discovery process using 3D cell culture models. The present review provides an overview of the techniques that can be employed to characterize and evaluate the efficacy of anticancer therapeutics in 3D tumor spheroids. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-11 2016-11-11T00:00:00Z 2018-03-21T15:40:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/4690 |
url |
http://hdl.handle.net/10400.6/4690 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.biotechadv.2016.11.002 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817549604324900864 |