Molinate quantification in environmental water by a glutathione-S-transferase based biosensor

• Autor(a) principal: Oliveira, Túlio I. S.
• Data de Publicação: 2013
• Outros Autores: Oliveira, Marcela, Viswanathan, Subramanian, Barroso, M. Fátima, Barreiros, Luísa, Nunes, O. C., Rodrigues, José A., Lima-Neto, Pedro de, Mazzetto, Selma E., Morais, Simone, Delerue-Matos, Cristina
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.22/3346
• topic: Glutathione-S-transferase; Enzymatic biosensor; Voltammetry; Molinate; Rice paddy field floodwater

A glutathione-S-transferase (GST)based biosensor was developed to quantify the thiocarbamate herbicide molinate in environmental water.The biosensor construction was based on GST immobilization onto a glassy carbon electrode via aminosilane–glutaraldehyde covalent attachment. The principle supporting the use of this biosensor consists of the GST inhibition process promoted by molinate. Differential pulse voltammetry was used to obtain a calibration curve for molinate concentration, ranging from 0.19 to 7.9 mgL -1 and presenting a detection limit of 0.064 mgL- 1. The developed biosensor is stable,and reusable during 15 days.The GST-based biosensor was successfully applied to quantify molinate in rice paddy field floodwater samples. The results achieved with the developed biosensor were in accordance with those obtained by high performance liquid chromatography. The proposed device is suitable for screening environmental water analysis and, since no sample preparation is required, it can be used in situ and in real-time measurements.