Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/23602 |
Resumo: | © 2015 The Society for Applied Microbiology |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydrationAtomic force microscopyFreeze-dryingLactobacillus plantarumWineZeta potential© 2015 The Society for Applied MicrobiologyAims: In this work, we evaluated freeze-drying damage at the surface level of oenological strain Lactobacillus plantarum UNQLp155, as well as its ability to grow in a synthetic wine with and without pre-acclimation. Methods and Results: Damage on cell surface was studied by flow cytometry, zeta potential and atomic force microscopy, and cell survival was analysed by plate count. Results showed that beside cells acclimated at lower ethanol concentration (6% v/v) became more susceptible to drying than nonacclimated ones, after rehydration they maintain their increased ability to grow in a synthetic wine. Acclimation at a higher ethanol concentration (10% v/v) produces several damages on the cell surface losing its ability to grow in a synthetic wine. Conclusions: In this work, we showed for the first time that sublethal alterations on bacterial surface induced by a pre-acclimation with a low ethanol concentration (6%), upon a freeze-drying process, result in a better bacterial adaptation to the stress conditions of wine-like medium, as well as to the preservation process. Significance and Impact of the Study: Understanding the adaptation to ethanol of oenological strains and their effects on the preservation process has a strong impact on winemaking process and allows to define the most appropriate conditions to obtain malolactic starters cultures.This work has been funded by grants from Universidad Nacional de Quilmes (Programa Microbiología Molecular Básica y Aplicaciones Biotecnológicas, Argentina), Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT – MINCyT, Argentina; PICTO-UNQ 2006 N-36474 and PICT SU 2012 N° 2804), Comisión Nacional de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-BA, Argentina) and Fundação para a Ciência e Tecnologia – Ministério da Educação e Ciência (FCTMEC, Portugal). Bravo-Ferrada, B.M. has a fellowship from the Consejo Nacional de Investigaciones Científicasy Técnicas (CONICET). AH and EET are members of the Carrera del Investigador Científico y Tecnológico (CONICET, Argentina). LS is member of the Research Career of Comisión de Investigaciones Científicas de la Provinciade Buenos Aires (CIC-BA).WileyRepositório da Universidade de LisboaBravo-Ferrada, B. M.Gonçalves, S.Semorile, L.Santos, N. C.Tymczyszyn, E. E.Hollmann, A.2016-05-04T15:32:41Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/23602engJournal of Applied Microbiology 118, 1409-14171364-507210.1111/jam.12796metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:11:43Zoai:repositorio.ul.pt:10451/23602Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:40:55.278622Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration |
title |
Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration |
spellingShingle |
Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration Bravo-Ferrada, B. M. Atomic force microscopy Freeze-drying Lactobacillus plantarum Wine Zeta potential |
title_short |
Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration |
title_full |
Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration |
title_fullStr |
Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration |
title_full_unstemmed |
Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration |
title_sort |
Study of surface damage on cell envelope assessed by AFM and flow cytometry of Lactobacillus plantarum exposed to ethanol and dehydration |
author |
Bravo-Ferrada, B. M. |
author_facet |
Bravo-Ferrada, B. M. Gonçalves, S. Semorile, L. Santos, N. C. Tymczyszyn, E. E. Hollmann, A. |
author_role |
author |
author2 |
Gonçalves, S. Semorile, L. Santos, N. C. Tymczyszyn, E. E. Hollmann, A. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Bravo-Ferrada, B. M. Gonçalves, S. Semorile, L. Santos, N. C. Tymczyszyn, E. E. Hollmann, A. |
dc.subject.por.fl_str_mv |
Atomic force microscopy Freeze-drying Lactobacillus plantarum Wine Zeta potential |
topic |
Atomic force microscopy Freeze-drying Lactobacillus plantarum Wine Zeta potential |
description |
© 2015 The Society for Applied Microbiology |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z 2016-05-04T15:32:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/23602 |
url |
http://hdl.handle.net/10451/23602 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Microbiology 118, 1409-1417 1364-5072 10.1111/jam.12796 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
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metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134320007315456 |