3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize

Detalhes bibliográficos
Autor(a) principal: Barroso, M. Fátima
Data de Publicação: 2015
Outros Autores: Freitas, Maria, Oliveira, M. Beatriz P.P., de-los-Santos-Álvarez, Noemí, Lobo-Castañón, María Jesús, Delerue-Matos, Cristina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.22/7253
Resumo: In the present work, the development of a genosensor for the event-specific detection of MON810 transgenic maize is proposed. Taking advantage of nanostructuration, a cost-effective three dimensional electrode was fabricated and a ternary monolayer containing a dithiol, a monothiol and the thiolated capture probe was optimized to minimize the unspecific signals. A sandwich format assay was selected as a way of precluding inefficient hybridization associated with stable secondary target structures. A comparison between the analytical performance of the Au nanostructured electrodes and commercially available screen-printed electrodes highlighted the superior performance of the nanostructured ones. Finally, the genosensor was effectively applied to detect the transgenic sequence in real samples, showing its potential for future quantitative analysis.
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spelling 3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maizeGMOGenosensorNanostructured 3D Au electrodesScreen-printed electrodesMON810 maizeIn the present work, the development of a genosensor for the event-specific detection of MON810 transgenic maize is proposed. Taking advantage of nanostructuration, a cost-effective three dimensional electrode was fabricated and a ternary monolayer containing a dithiol, a monothiol and the thiolated capture probe was optimized to minimize the unspecific signals. A sandwich format assay was selected as a way of precluding inefficient hybridization associated with stable secondary target structures. A comparison between the analytical performance of the Au nanostructured electrodes and commercially available screen-printed electrodes highlighted the superior performance of the nanostructured ones. Finally, the genosensor was effectively applied to detect the transgenic sequence in real samples, showing its potential for future quantitative analysis.ElsevierRepositório Científico do Instituto Politécnico do PortoBarroso, M. FátimaFreitas, MariaOliveira, M. Beatriz P.P.de-los-Santos-Álvarez, NoemíLobo-Castañón, María JesúsDelerue-Matos, Cristina2015-12-23T16:00:52Z2015-032015-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/7253eng10.1016/j.talanta.2014.10.017info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:47:38Zoai:recipp.ipp.pt:10400.22/7253Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:27:45.352308Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv 3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize
title 3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize
spellingShingle 3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize
Barroso, M. Fátima
GMO
Genosensor
Nanostructured 3D Au electrodes
Screen-printed electrodes
MON810 maize
title_short 3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize
title_full 3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize
title_fullStr 3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize
title_full_unstemmed 3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize
title_sort 3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize
author Barroso, M. Fátima
author_facet Barroso, M. Fátima
Freitas, Maria
Oliveira, M. Beatriz P.P.
de-los-Santos-Álvarez, Noemí
Lobo-Castañón, María Jesús
Delerue-Matos, Cristina
author_role author
author2 Freitas, Maria
Oliveira, M. Beatriz P.P.
de-los-Santos-Álvarez, Noemí
Lobo-Castañón, María Jesús
Delerue-Matos, Cristina
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico do Porto
dc.contributor.author.fl_str_mv Barroso, M. Fátima
Freitas, Maria
Oliveira, M. Beatriz P.P.
de-los-Santos-Álvarez, Noemí
Lobo-Castañón, María Jesús
Delerue-Matos, Cristina
dc.subject.por.fl_str_mv GMO
Genosensor
Nanostructured 3D Au electrodes
Screen-printed electrodes
MON810 maize
topic GMO
Genosensor
Nanostructured 3D Au electrodes
Screen-printed electrodes
MON810 maize
description In the present work, the development of a genosensor for the event-specific detection of MON810 transgenic maize is proposed. Taking advantage of nanostructuration, a cost-effective three dimensional electrode was fabricated and a ternary monolayer containing a dithiol, a monothiol and the thiolated capture probe was optimized to minimize the unspecific signals. A sandwich format assay was selected as a way of precluding inefficient hybridization associated with stable secondary target structures. A comparison between the analytical performance of the Au nanostructured electrodes and commercially available screen-printed electrodes highlighted the superior performance of the nanostructured ones. Finally, the genosensor was effectively applied to detect the transgenic sequence in real samples, showing its potential for future quantitative analysis.
publishDate 2015
dc.date.none.fl_str_mv 2015-12-23T16:00:52Z
2015-03
2015-03-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.22/7253
url http://hdl.handle.net/10400.22/7253
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.talanta.2014.10.017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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