3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.22/7253 |
Resumo: | In the present work, the development of a genosensor for the event-specific detection of MON810 transgenic maize is proposed. Taking advantage of nanostructuration, a cost-effective three dimensional electrode was fabricated and a ternary monolayer containing a dithiol, a monothiol and the thiolated capture probe was optimized to minimize the unspecific signals. A sandwich format assay was selected as a way of precluding inefficient hybridization associated with stable secondary target structures. A comparison between the analytical performance of the Au nanostructured electrodes and commercially available screen-printed electrodes highlighted the superior performance of the nanostructured ones. Finally, the genosensor was effectively applied to detect the transgenic sequence in real samples, showing its potential for future quantitative analysis. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
spelling |
3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maizeGMOGenosensorNanostructured 3D Au electrodesScreen-printed electrodesMON810 maizeIn the present work, the development of a genosensor for the event-specific detection of MON810 transgenic maize is proposed. Taking advantage of nanostructuration, a cost-effective three dimensional electrode was fabricated and a ternary monolayer containing a dithiol, a monothiol and the thiolated capture probe was optimized to minimize the unspecific signals. A sandwich format assay was selected as a way of precluding inefficient hybridization associated with stable secondary target structures. A comparison between the analytical performance of the Au nanostructured electrodes and commercially available screen-printed electrodes highlighted the superior performance of the nanostructured ones. Finally, the genosensor was effectively applied to detect the transgenic sequence in real samples, showing its potential for future quantitative analysis.ElsevierRepositório Científico do Instituto Politécnico do PortoBarroso, M. FátimaFreitas, MariaOliveira, M. Beatriz P.P.de-los-Santos-Álvarez, NoemíLobo-Castañón, María JesúsDelerue-Matos, Cristina2015-12-23T16:00:52Z2015-032015-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.22/7253eng10.1016/j.talanta.2014.10.017info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-13T12:47:38Zoai:recipp.ipp.pt:10400.22/7253Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:27:45.352308Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize |
title |
3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize |
spellingShingle |
3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize Barroso, M. Fátima GMO Genosensor Nanostructured 3D Au electrodes Screen-printed electrodes MON810 maize |
title_short |
3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize |
title_full |
3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize |
title_fullStr |
3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize |
title_full_unstemmed |
3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize |
title_sort |
3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize |
author |
Barroso, M. Fátima |
author_facet |
Barroso, M. Fátima Freitas, Maria Oliveira, M. Beatriz P.P. de-los-Santos-Álvarez, Noemí Lobo-Castañón, María Jesús Delerue-Matos, Cristina |
author_role |
author |
author2 |
Freitas, Maria Oliveira, M. Beatriz P.P. de-los-Santos-Álvarez, Noemí Lobo-Castañón, María Jesús Delerue-Matos, Cristina |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico do Porto |
dc.contributor.author.fl_str_mv |
Barroso, M. Fátima Freitas, Maria Oliveira, M. Beatriz P.P. de-los-Santos-Álvarez, Noemí Lobo-Castañón, María Jesús Delerue-Matos, Cristina |
dc.subject.por.fl_str_mv |
GMO Genosensor Nanostructured 3D Au electrodes Screen-printed electrodes MON810 maize |
topic |
GMO Genosensor Nanostructured 3D Au electrodes Screen-printed electrodes MON810 maize |
description |
In the present work, the development of a genosensor for the event-specific detection of MON810 transgenic maize is proposed. Taking advantage of nanostructuration, a cost-effective three dimensional electrode was fabricated and a ternary monolayer containing a dithiol, a monothiol and the thiolated capture probe was optimized to minimize the unspecific signals. A sandwich format assay was selected as a way of precluding inefficient hybridization associated with stable secondary target structures. A comparison between the analytical performance of the Au nanostructured electrodes and commercially available screen-printed electrodes highlighted the superior performance of the nanostructured ones. Finally, the genosensor was effectively applied to detect the transgenic sequence in real samples, showing its potential for future quantitative analysis. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-23T16:00:52Z 2015-03 2015-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.22/7253 |
url |
http://hdl.handle.net/10400.22/7253 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.talanta.2014.10.017 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131373448986624 |