Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Liliana Andreia Amaro
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/12207
Resumo: Dissertação para obtenção do Grau de Mestre em Engenharia Química e Bioquímica
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spelling Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technologySupercritical fluid extractionLavandula sppMentha sppTerpenesAntiproliferative activityColorectal cancerDissertação para obtenção do Grau de Mestre em Engenharia Química e BioquímicaThe knowledge of the healing power of some species of plants is ancient. Nowadays, the preference for natural products has increased, and with it, the interest in recover and isolate bioactive phytochemicals with potential health benefits. The main goal of this thesis was to isolate anticancer bioactive compounds, namely terpenes and fatty acids, from Portuguese Lavandula (L. angustifolia and L. stoechas) and Mentha species (M. spicata, M. piperita var. citrata and M. pulegium), using supercritical fluid extraction (SFE). All extractions were performed at 50ºC and 250 bar in a two step methodology: a first step with supercritical CO2, during 60 minutes, followed by a second step, with a mixture of CO2 and ethanol (90:10 %v/v), for 180 minutes. Different fractions of the extracts were recovered every 30 minutes, and the resulting samples were characterized in terms of global yield (% g/g), screened for the presence of terpenes, by thin layer chromatography (TLC) and analyzed by gas chromatography coupled with mass spectrometry detection (GC-MS). Furthermore, ORAC, HORAC and HOSC assays were conducted for the determination of antioxidant activity. Cell-based assays in human HT-29 colorectal adenocarcinoma cells were also performed, in order to determine the potential of the extracts as cancer cell-growth inhibitors. The fractions of the extract of M. piperita var. citrata presented the highest antioxidant activity, whereas the fractions of L. angustifolia, L. stoechas and M. spicata extracts revealed to be the most promising colorectal cancer cell-growth inhibitors. The bioactive responses were correlated with the phytochemical composition of the extracts, and the main compounds, such as camphor, fenchone, carvone, linoleic acid, among others, were highlighted. However, it is possible that the bioactivities were due to synergistic effects between several compounds. Results of this thesis demonstrated that SFE is a reliable tool to extract anticancer bioactive components from some Lavandula and Mentha plants.Faculdade de Ciências e TecnologiaDuarte, CatarinaSerra, AnaRUNRodrigues, Liliana Andreia Amaro2014-06-20T15:58:24Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/12207enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:47:05Zoai:run.unl.pt:10362/12207Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:20:48.375381Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology
title Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology
spellingShingle Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology
Rodrigues, Liliana Andreia Amaro
Supercritical fluid extraction
Lavandula spp
Mentha spp
Terpenes
Antiproliferative activity
Colorectal cancer
title_short Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology
title_full Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology
title_fullStr Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology
title_full_unstemmed Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology
title_sort Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology
author Rodrigues, Liliana Andreia Amaro
author_facet Rodrigues, Liliana Andreia Amaro
author_role author
dc.contributor.none.fl_str_mv Duarte, Catarina
Serra, Ana
RUN
dc.contributor.author.fl_str_mv Rodrigues, Liliana Andreia Amaro
dc.subject.por.fl_str_mv Supercritical fluid extraction
Lavandula spp
Mentha spp
Terpenes
Antiproliferative activity
Colorectal cancer
topic Supercritical fluid extraction
Lavandula spp
Mentha spp
Terpenes
Antiproliferative activity
Colorectal cancer
description Dissertação para obtenção do Grau de Mestre em Engenharia Química e Bioquímica
publishDate 2014
dc.date.none.fl_str_mv 2014-06-20T15:58:24Z
2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/12207
url http://hdl.handle.net/10362/12207
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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