Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/12207 |
Resumo: | Dissertação para obtenção do Grau de Mestre em Engenharia Química e Bioquímica |
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Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technologySupercritical fluid extractionLavandula sppMentha sppTerpenesAntiproliferative activityColorectal cancerDissertação para obtenção do Grau de Mestre em Engenharia Química e BioquímicaThe knowledge of the healing power of some species of plants is ancient. Nowadays, the preference for natural products has increased, and with it, the interest in recover and isolate bioactive phytochemicals with potential health benefits. The main goal of this thesis was to isolate anticancer bioactive compounds, namely terpenes and fatty acids, from Portuguese Lavandula (L. angustifolia and L. stoechas) and Mentha species (M. spicata, M. piperita var. citrata and M. pulegium), using supercritical fluid extraction (SFE). All extractions were performed at 50ºC and 250 bar in a two step methodology: a first step with supercritical CO2, during 60 minutes, followed by a second step, with a mixture of CO2 and ethanol (90:10 %v/v), for 180 minutes. Different fractions of the extracts were recovered every 30 minutes, and the resulting samples were characterized in terms of global yield (% g/g), screened for the presence of terpenes, by thin layer chromatography (TLC) and analyzed by gas chromatography coupled with mass spectrometry detection (GC-MS). Furthermore, ORAC, HORAC and HOSC assays were conducted for the determination of antioxidant activity. Cell-based assays in human HT-29 colorectal adenocarcinoma cells were also performed, in order to determine the potential of the extracts as cancer cell-growth inhibitors. The fractions of the extract of M. piperita var. citrata presented the highest antioxidant activity, whereas the fractions of L. angustifolia, L. stoechas and M. spicata extracts revealed to be the most promising colorectal cancer cell-growth inhibitors. The bioactive responses were correlated with the phytochemical composition of the extracts, and the main compounds, such as camphor, fenchone, carvone, linoleic acid, among others, were highlighted. However, it is possible that the bioactivities were due to synergistic effects between several compounds. Results of this thesis demonstrated that SFE is a reliable tool to extract anticancer bioactive components from some Lavandula and Mentha plants.Faculdade de Ciências e TecnologiaDuarte, CatarinaSerra, AnaRUNRodrigues, Liliana Andreia Amaro2014-06-20T15:58:24Z20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/12207enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:47:05Zoai:run.unl.pt:10362/12207Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:20:48.375381Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology |
title |
Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology |
spellingShingle |
Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology Rodrigues, Liliana Andreia Amaro Supercritical fluid extraction Lavandula spp Mentha spp Terpenes Antiproliferative activity Colorectal cancer |
title_short |
Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology |
title_full |
Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology |
title_fullStr |
Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology |
title_full_unstemmed |
Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology |
title_sort |
Development of a "green process" for the isolation of natural functional extracts with anti-cancer activity - Application of high-pressure technology |
author |
Rodrigues, Liliana Andreia Amaro |
author_facet |
Rodrigues, Liliana Andreia Amaro |
author_role |
author |
dc.contributor.none.fl_str_mv |
Duarte, Catarina Serra, Ana RUN |
dc.contributor.author.fl_str_mv |
Rodrigues, Liliana Andreia Amaro |
dc.subject.por.fl_str_mv |
Supercritical fluid extraction Lavandula spp Mentha spp Terpenes Antiproliferative activity Colorectal cancer |
topic |
Supercritical fluid extraction Lavandula spp Mentha spp Terpenes Antiproliferative activity Colorectal cancer |
description |
Dissertação para obtenção do Grau de Mestre em Engenharia Química e Bioquímica |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-20T15:58:24Z 2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/12207 |
url |
http://hdl.handle.net/10362/12207 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Faculdade de Ciências e Tecnologia |
publisher.none.fl_str_mv |
Faculdade de Ciências e Tecnologia |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137849158664192 |