Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides

Detalhes bibliográficos
Autor(a) principal: Maranha, Ana
Data de Publicação: 2023
Outros Autores: Costa, Mafalda, Ripoll-Rozada, Jorge, Manso, José A., Miranda, Vanessa, Mendes, Vera M., Manadas, Bruno, Macedo-Ribeiro, Sandra, Ventura, M. Rita, Pereira, Pedro José Barbosa, Empadinhas, Nuno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/165714
Resumo: Funding Information: This work was funded by Portuguese national funds via FCT—Fundação para a Ciência e a Tecnologia through projects PTDC/BIA-MIC/0122/2021, UIDB/04539/2020, UIDP/04539/2020 and LA/P/0058/2020; through PhD Fellowship SFRH/BD/101191/2014 (to M.C.); through contract POCI-01-0145-FEDER-029221 (to A. M.); through contract DL 57/2016/CP1355/CT0011 (to J.R.-R.) and by the European Social Fund through Programa Operacional Capital Humano in the form of Postdoctoral Fellowship SFRH/BPD/108004/2015 (to J.R.-R.) and by the European Regional Development Fund (FEDER) through the COMPETE 2020-Operational Programme for Competitiveness and Internationalization (POCI), Portugal 2020 in the form of grant POCI-01-0145-FEDER-029221, and the National Mass Spectrometry Network (RNEM) under the contract POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013). M.R.V. acknowledges MostMicro Research Unit, financially supported by LISBOA-01-0145-FEDER-007660 funded by FEDER funds through COMPETE2020 (POCI) and by national funds through FCT. The NMR data was acquired at CERMAX, ITQB-NOVA, Oeiras, Portugal with equipment funded by FCT, project AAC 01/SAICT/2016. We thank Igor Tiago (Centre for Functional Ecology, University of Coimbra) for support with computation and bioinformatic analyses. We thank ALBA Synchrotron (Cerdanyola del Vallès, Spain) and the European Synchrotron Radiation Facility (Grenoble, France) for provision of synchrotron radiation facilities, and their staff for help with data collection. The support of the X-ray Crystallography scientific platform of i3S (Porto, Portugal) is also acknowledged. Funding Information: This work was funded by Portuguese national funds via FCT—Fundação para a Ciência e a Tecnologia through projects PTDC/BIA-MIC/0122/2021, UIDB/04539/2020, UIDP/04539/2020 and LA/P/0058/2020; through PhD Fellowship SFRH/BD/101191/2014 (to M.C.); through contract POCI-01-0145-FEDER-029221 (to A. M.); through contract DL 57/2016/CP1355/CT0011 (to J.R.-R.) and by the European Social Fund through Programa Operacional Capital Humano in the form of Postdoctoral Fellowship SFRH/BPD/108004/2015 (to J.R.-R.) and by the European Regional Development Fund (FEDER) through the COMPETE 2020-Operational Programme for Competitiveness and Internationalization (POCI), Portugal 2020 in the form of grant POCI-01-0145-FEDER-029221, and the National Mass Spectrometry Network (RNEM) under the contract POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013). M.R.V. acknowledges MostMicro Research Unit, financially supported by LISBOA-01-0145-FEDER-007660 funded by FEDER funds through COMPETE2020 (POCI) and by national funds through FCT. The NMR data was acquired at CERMAX, ITQB-NOVA, Oeiras, Portugal with equipment funded by FCT, project AAC 01/SAICT/2016. We thank Igor Tiago (Centre for Functional Ecology, University of Coimbra) for support with computation and bioinformatic analyses. We thank ALBA Synchrotron (Cerdanyola del Vallès, Spain) and the European Synchrotron Radiation Facility (Grenoble, France) for provision of synchrotron radiation facilities, and their staff for help with data collection. The support of the X-ray Crystallography scientific platform of i3S (Porto, Portugal) is also acknowledged. Publisher Copyright: © 2023, The Author(s).
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network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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spelling Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharidesMedicine (miscellaneous)Biochemistry, Genetics and Molecular Biology(all)Agricultural and Biological Sciences(all)Funding Information: This work was funded by Portuguese national funds via FCT—Fundação para a Ciência e a Tecnologia through projects PTDC/BIA-MIC/0122/2021, UIDB/04539/2020, UIDP/04539/2020 and LA/P/0058/2020; through PhD Fellowship SFRH/BD/101191/2014 (to M.C.); through contract POCI-01-0145-FEDER-029221 (to A. M.); through contract DL 57/2016/CP1355/CT0011 (to J.R.-R.) and by the European Social Fund through Programa Operacional Capital Humano in the form of Postdoctoral Fellowship SFRH/BPD/108004/2015 (to J.R.-R.) and by the European Regional Development Fund (FEDER) through the COMPETE 2020-Operational Programme for Competitiveness and Internationalization (POCI), Portugal 2020 in the form of grant POCI-01-0145-FEDER-029221, and the National Mass Spectrometry Network (RNEM) under the contract POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013). M.R.V. acknowledges MostMicro Research Unit, financially supported by LISBOA-01-0145-FEDER-007660 funded by FEDER funds through COMPETE2020 (POCI) and by national funds through FCT. The NMR data was acquired at CERMAX, ITQB-NOVA, Oeiras, Portugal with equipment funded by FCT, project AAC 01/SAICT/2016. We thank Igor Tiago (Centre for Functional Ecology, University of Coimbra) for support with computation and bioinformatic analyses. We thank ALBA Synchrotron (Cerdanyola del Vallès, Spain) and the European Synchrotron Radiation Facility (Grenoble, France) for provision of synchrotron radiation facilities, and their staff for help with data collection. The support of the X-ray Crystallography scientific platform of i3S (Porto, Portugal) is also acknowledged. Funding Information: This work was funded by Portuguese national funds via FCT—Fundação para a Ciência e a Tecnologia through projects PTDC/BIA-MIC/0122/2021, UIDB/04539/2020, UIDP/04539/2020 and LA/P/0058/2020; through PhD Fellowship SFRH/BD/101191/2014 (to M.C.); through contract POCI-01-0145-FEDER-029221 (to A. M.); through contract DL 57/2016/CP1355/CT0011 (to J.R.-R.) and by the European Social Fund through Programa Operacional Capital Humano in the form of Postdoctoral Fellowship SFRH/BPD/108004/2015 (to J.R.-R.) and by the European Regional Development Fund (FEDER) through the COMPETE 2020-Operational Programme for Competitiveness and Internationalization (POCI), Portugal 2020 in the form of grant POCI-01-0145-FEDER-029221, and the National Mass Spectrometry Network (RNEM) under the contract POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013). M.R.V. acknowledges MostMicro Research Unit, financially supported by LISBOA-01-0145-FEDER-007660 funded by FEDER funds through COMPETE2020 (POCI) and by national funds through FCT. The NMR data was acquired at CERMAX, ITQB-NOVA, Oeiras, Portugal with equipment funded by FCT, project AAC 01/SAICT/2016. We thank Igor Tiago (Centre for Functional Ecology, University of Coimbra) for support with computation and bioinformatic analyses. We thank ALBA Synchrotron (Cerdanyola del Vallès, Spain) and the European Synchrotron Radiation Facility (Grenoble, France) for provision of synchrotron radiation facilities, and their staff for help with data collection. The support of the X-ray Crystallography scientific platform of i3S (Porto, Portugal) is also acknowledged. Publisher Copyright: © 2023, The Author(s).The steep increase in nontuberculous mycobacteria (NTM) infections makes understanding their unique physiology an urgent health priority. NTM synthesize two polysaccharides proposed to modulate fatty acid metabolism: the ubiquitous 6-O-methylglucose lipopolysaccharide, and the 3-O-methylmannose polysaccharide (MMP) so far detected in rapidly growing mycobacteria. The recent identification of a unique MMP methyltransferase implicated the adjacent genes in MMP biosynthesis. We report a wide distribution of this gene cluster in NTM, including slowly growing mycobacteria such as Mycobacterium avium, which we reveal to produce MMP. Using a combination of MMP purification and chemoenzymatic syntheses of intermediates, we identified the biosynthetic mechanism of MMP, relying on two enzymes that we characterized biochemically and structurally: a previously undescribed α–endomannosidase that hydrolyses MMP into defined-sized mannoligosaccharides that prime the elongation of new daughter MMP chains by a rare α-(1→4)-mannosyltransferase. Therefore, MMP biogenesis occurs through a partially conservative replication mechanism, whose disruption affected mycobacterial growth rate at low temperature.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNMaranha, AnaCosta, MafaldaRipoll-Rozada, JorgeManso, José A.Miranda, VanessaMendes, Vera M.Manadas, BrunoMacedo-Ribeiro, SandraVentura, M. RitaPereira, Pedro José BarbosaEmpadinhas, Nuno2024-04-02T23:18:22Z2023-122023-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/165714engPURE: 72012992https://doi.org/10.1038/s42003-023-04448-3info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-08-05T01:33:46Zoai:run.unl.pt:10362/165714Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-08-05T01:33:46Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides
title Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides
spellingShingle Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides
Maranha, Ana
Medicine (miscellaneous)
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
title_short Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides
title_full Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides
title_fullStr Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides
title_full_unstemmed Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides
title_sort Self-recycling and partially conservative replication of mycobacterial methylmannose polysaccharides
author Maranha, Ana
author_facet Maranha, Ana
Costa, Mafalda
Ripoll-Rozada, Jorge
Manso, José A.
Miranda, Vanessa
Mendes, Vera M.
Manadas, Bruno
Macedo-Ribeiro, Sandra
Ventura, M. Rita
Pereira, Pedro José Barbosa
Empadinhas, Nuno
author_role author
author2 Costa, Mafalda
Ripoll-Rozada, Jorge
Manso, José A.
Miranda, Vanessa
Mendes, Vera M.
Manadas, Bruno
Macedo-Ribeiro, Sandra
Ventura, M. Rita
Pereira, Pedro José Barbosa
Empadinhas, Nuno
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Maranha, Ana
Costa, Mafalda
Ripoll-Rozada, Jorge
Manso, José A.
Miranda, Vanessa
Mendes, Vera M.
Manadas, Bruno
Macedo-Ribeiro, Sandra
Ventura, M. Rita
Pereira, Pedro José Barbosa
Empadinhas, Nuno
dc.subject.por.fl_str_mv Medicine (miscellaneous)
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
topic Medicine (miscellaneous)
Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)
description Funding Information: This work was funded by Portuguese national funds via FCT—Fundação para a Ciência e a Tecnologia through projects PTDC/BIA-MIC/0122/2021, UIDB/04539/2020, UIDP/04539/2020 and LA/P/0058/2020; through PhD Fellowship SFRH/BD/101191/2014 (to M.C.); through contract POCI-01-0145-FEDER-029221 (to A. M.); through contract DL 57/2016/CP1355/CT0011 (to J.R.-R.) and by the European Social Fund through Programa Operacional Capital Humano in the form of Postdoctoral Fellowship SFRH/BPD/108004/2015 (to J.R.-R.) and by the European Regional Development Fund (FEDER) through the COMPETE 2020-Operational Programme for Competitiveness and Internationalization (POCI), Portugal 2020 in the form of grant POCI-01-0145-FEDER-029221, and the National Mass Spectrometry Network (RNEM) under the contract POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013). M.R.V. acknowledges MostMicro Research Unit, financially supported by LISBOA-01-0145-FEDER-007660 funded by FEDER funds through COMPETE2020 (POCI) and by national funds through FCT. The NMR data was acquired at CERMAX, ITQB-NOVA, Oeiras, Portugal with equipment funded by FCT, project AAC 01/SAICT/2016. We thank Igor Tiago (Centre for Functional Ecology, University of Coimbra) for support with computation and bioinformatic analyses. We thank ALBA Synchrotron (Cerdanyola del Vallès, Spain) and the European Synchrotron Radiation Facility (Grenoble, France) for provision of synchrotron radiation facilities, and their staff for help with data collection. The support of the X-ray Crystallography scientific platform of i3S (Porto, Portugal) is also acknowledged. Funding Information: This work was funded by Portuguese national funds via FCT—Fundação para a Ciência e a Tecnologia through projects PTDC/BIA-MIC/0122/2021, UIDB/04539/2020, UIDP/04539/2020 and LA/P/0058/2020; through PhD Fellowship SFRH/BD/101191/2014 (to M.C.); through contract POCI-01-0145-FEDER-029221 (to A. M.); through contract DL 57/2016/CP1355/CT0011 (to J.R.-R.) and by the European Social Fund through Programa Operacional Capital Humano in the form of Postdoctoral Fellowship SFRH/BPD/108004/2015 (to J.R.-R.) and by the European Regional Development Fund (FEDER) through the COMPETE 2020-Operational Programme for Competitiveness and Internationalization (POCI), Portugal 2020 in the form of grant POCI-01-0145-FEDER-029221, and the National Mass Spectrometry Network (RNEM) under the contract POCI-01-0145-FEDER-402-022125 (ref.: ROTEIRO/0028/2013). M.R.V. acknowledges MostMicro Research Unit, financially supported by LISBOA-01-0145-FEDER-007660 funded by FEDER funds through COMPETE2020 (POCI) and by national funds through FCT. The NMR data was acquired at CERMAX, ITQB-NOVA, Oeiras, Portugal with equipment funded by FCT, project AAC 01/SAICT/2016. We thank Igor Tiago (Centre for Functional Ecology, University of Coimbra) for support with computation and bioinformatic analyses. We thank ALBA Synchrotron (Cerdanyola del Vallès, Spain) and the European Synchrotron Radiation Facility (Grenoble, France) for provision of synchrotron radiation facilities, and their staff for help with data collection. The support of the X-ray Crystallography scientific platform of i3S (Porto, Portugal) is also acknowledged. Publisher Copyright: © 2023, The Author(s).
publishDate 2023
dc.date.none.fl_str_mv 2023-12
2023-12-01T00:00:00Z
2024-04-02T23:18:22Z
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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