Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/31112 |
Resumo: | The effectiveness of polyhydroxyalkanoates (PHAs) production process from a waste stream is determined by the selection of a suitable mixed microbial culture (MMC). In this work, a feedstock from the paper industry, hardwood spent sulfite liquor (HSSL), supplemented with short-chain organic acids (SCOAs) to simulate a fermented effluent, was used as substrate to enrich a MMC in PHA-storing microorganisms. A stable culture was quickly established, and during the accumulation step the selected MMC reached a maximum PHA content of 34.6% (3HB:3HV-76:24). The bacterial community was analyzed through FISH analysis. Bacteria belonging to the four main classes were identified: Betaproteobacteria (44.7 ± 2.7%), Alphaproteobacteria (13.6 ± 1.3%) and Gammaproteobacteria (2.40 ± 1.1%) and Bacteroidetes (9.20 ± 3.8%). Inside the Betaproteobacteria class, Acidovorax (71%) was the dominant genus. |
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Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organismsPolyhydroxyalkanoatesMixed microbial culturesHardwood spent sulfite liquorShort-chain organic acidsFluorescence in situ Hybridization (FISH)The effectiveness of polyhydroxyalkanoates (PHAs) production process from a waste stream is determined by the selection of a suitable mixed microbial culture (MMC). In this work, a feedstock from the paper industry, hardwood spent sulfite liquor (HSSL), supplemented with short-chain organic acids (SCOAs) to simulate a fermented effluent, was used as substrate to enrich a MMC in PHA-storing microorganisms. A stable culture was quickly established, and during the accumulation step the selected MMC reached a maximum PHA content of 34.6% (3HB:3HV-76:24). The bacterial community was analyzed through FISH analysis. Bacteria belonging to the four main classes were identified: Betaproteobacteria (44.7 ± 2.7%), Alphaproteobacteria (13.6 ± 1.3%) and Gammaproteobacteria (2.40 ± 1.1%) and Bacteroidetes (9.20 ± 3.8%). Inside the Betaproteobacteria class, Acidovorax (71%) was the dominant genus.MDPI2021-04-06T15:40:36Z2018-07-24T00:00:00Z2018-07-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/31112eng10.3390/fermentation4030058Queirós, DiogoRangel, CatarinaLemos, Paulo CostaRossetti, SimonaSerafim, Luísa Seuanesinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:00:05Zoai:ria.ua.pt:10773/31112Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:03:04.507175Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms |
title |
Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms |
spellingShingle |
Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms Queirós, Diogo Polyhydroxyalkanoates Mixed microbial cultures Hardwood spent sulfite liquor Short-chain organic acids Fluorescence in situ Hybridization (FISH) |
title_short |
Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms |
title_full |
Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms |
title_fullStr |
Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms |
title_full_unstemmed |
Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms |
title_sort |
Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms |
author |
Queirós, Diogo |
author_facet |
Queirós, Diogo Rangel, Catarina Lemos, Paulo Costa Rossetti, Simona Serafim, Luísa Seuanes |
author_role |
author |
author2 |
Rangel, Catarina Lemos, Paulo Costa Rossetti, Simona Serafim, Luísa Seuanes |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Queirós, Diogo Rangel, Catarina Lemos, Paulo Costa Rossetti, Simona Serafim, Luísa Seuanes |
dc.subject.por.fl_str_mv |
Polyhydroxyalkanoates Mixed microbial cultures Hardwood spent sulfite liquor Short-chain organic acids Fluorescence in situ Hybridization (FISH) |
topic |
Polyhydroxyalkanoates Mixed microbial cultures Hardwood spent sulfite liquor Short-chain organic acids Fluorescence in situ Hybridization (FISH) |
description |
The effectiveness of polyhydroxyalkanoates (PHAs) production process from a waste stream is determined by the selection of a suitable mixed microbial culture (MMC). In this work, a feedstock from the paper industry, hardwood spent sulfite liquor (HSSL), supplemented with short-chain organic acids (SCOAs) to simulate a fermented effluent, was used as substrate to enrich a MMC in PHA-storing microorganisms. A stable culture was quickly established, and during the accumulation step the selected MMC reached a maximum PHA content of 34.6% (3HB:3HV-76:24). The bacterial community was analyzed through FISH analysis. Bacteria belonging to the four main classes were identified: Betaproteobacteria (44.7 ± 2.7%), Alphaproteobacteria (13.6 ± 1.3%) and Gammaproteobacteria (2.40 ± 1.1%) and Bacteroidetes (9.20 ± 3.8%). Inside the Betaproteobacteria class, Acidovorax (71%) was the dominant genus. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-07-24T00:00:00Z 2018-07-24 2021-04-06T15:40:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/31112 |
url |
http://hdl.handle.net/10773/31112 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.3390/fermentation4030058 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799137686324248576 |