Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk

Detalhes bibliográficos
Autor(a) principal: Marques, Ana
Data de Publicação: 2019
Outros Autores: Veigas, Bruno, Araújo, Andreia, Pagará, Beatriz, Baptista, Pedro Viana, Águas, Hugo, Martins, Rodrigo, Fortunato, Elvira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/146963
Resumo: PTDC/CTM-NAN/2912/2014. PTDC/AAG-TEC/5400/2014. POCI-01-0145-FEDER-016637. POCI-01-0145-FEDER-007688. UID/CTM/50025/2019. FCT/MCTES SFRH/BD/115173/2016 and SFRH/BPD/124311/2016 .
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spelling Paper-Based SERS Platform for One-Step Screening of Tetracycline in MilkGeneralPTDC/CTM-NAN/2912/2014. PTDC/AAG-TEC/5400/2014. POCI-01-0145-FEDER-016637. POCI-01-0145-FEDER-007688. UID/CTM/50025/2019. FCT/MCTES SFRH/BD/115173/2016 and SFRH/BPD/124311/2016 .Throughout the last decade, the expansion of food testing has been gradually moving towards ordinary high throughput screening methods performed on-site. The demand for point-of-care testing, able to distinguish molecular signatures with high accuracy, sensitivity and specificity has been significantly increasing. This new requirement relies on the on-site detection and monitorization of molecular signatures suitable for the surveillance of food production and processing. The widespread use of antibiotics has contributed to disease control of livestock but has also created problems for the dairy industry and consumers. Its therapeutic and subtherapeutic use has increased the risk of contamination in milk in enough concentrations to cause economic losses to the dairy industry and have a health impact in highly sensitive individuals. This study focuses on the development of a simple Surface-Enhanced Raman Spectroscopy (SERS) method for fast high throughput screening of tetracycline (TET) in milk. For this, we integrate a paper-based low-cost, fully recyclable and highly stable SERS platform, with a minimal sample preparation protocol. A two-microliter sample of milk solutions spiked with TET (from 0.01 to 1000 ppm) is dried on a silver nanoparticle coated cardboard substrate and measured via a Raman spectrophotometer. The SERS substrate showed to be extremely stable with a shelf life of several months. A global spectrum principal component analysis approach was used to test all the detected vibrational modes and their correlation with TET concentration. Peak intensity ratios (455 cm−1/1280 cm−1 and 874 cm−1/1397 cm−1) were found to be correlated with TET concentrations in milk, achieving a sensitivity as low as 0.1 ppm. Results indicate that this SERS method combined with portable Raman spectrometer is a potential tool that can be used on-site for the monitoring of TET residues and other antibiotics.CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DCM - Departamento de Ciência dos MateriaisUCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNMarques, AnaVeigas, BrunoAraújo, AndreiaPagará, BeatrizBaptista, Pedro VianaÁguas, HugoMartins, RodrigoFortunato, Elvira2023-01-04T22:14:16Z2019-12-012019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/146963eng2045-2322PURE: 17189406https://doi.org/10.1038/s41598-019-54380-yinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:27:56Zoai:run.unl.pt:10362/146963Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:43.424660Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk
title Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk
spellingShingle Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk
Marques, Ana
General
title_short Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk
title_full Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk
title_fullStr Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk
title_full_unstemmed Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk
title_sort Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk
author Marques, Ana
author_facet Marques, Ana
Veigas, Bruno
Araújo, Andreia
Pagará, Beatriz
Baptista, Pedro Viana
Águas, Hugo
Martins, Rodrigo
Fortunato, Elvira
author_role author
author2 Veigas, Bruno
Araújo, Andreia
Pagará, Beatriz
Baptista, Pedro Viana
Águas, Hugo
Martins, Rodrigo
Fortunato, Elvira
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
DCM - Departamento de Ciência dos Materiais
UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Marques, Ana
Veigas, Bruno
Araújo, Andreia
Pagará, Beatriz
Baptista, Pedro Viana
Águas, Hugo
Martins, Rodrigo
Fortunato, Elvira
dc.subject.por.fl_str_mv General
topic General
description PTDC/CTM-NAN/2912/2014. PTDC/AAG-TEC/5400/2014. POCI-01-0145-FEDER-016637. POCI-01-0145-FEDER-007688. UID/CTM/50025/2019. FCT/MCTES SFRH/BD/115173/2016 and SFRH/BPD/124311/2016 .
publishDate 2019
dc.date.none.fl_str_mv 2019-12-01
2019-12-01T00:00:00Z
2023-01-04T22:14:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/146963
url http://hdl.handle.net/10362/146963
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2045-2322
PURE: 17189406
https://doi.org/10.1038/s41598-019-54380-y
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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