Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/146963 |
Resumo: | PTDC/CTM-NAN/2912/2014. PTDC/AAG-TEC/5400/2014. POCI-01-0145-FEDER-016637. POCI-01-0145-FEDER-007688. UID/CTM/50025/2019. FCT/MCTES SFRH/BD/115173/2016 and SFRH/BPD/124311/2016 . |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Paper-Based SERS Platform for One-Step Screening of Tetracycline in MilkGeneralPTDC/CTM-NAN/2912/2014. PTDC/AAG-TEC/5400/2014. POCI-01-0145-FEDER-016637. POCI-01-0145-FEDER-007688. UID/CTM/50025/2019. FCT/MCTES SFRH/BD/115173/2016 and SFRH/BPD/124311/2016 .Throughout the last decade, the expansion of food testing has been gradually moving towards ordinary high throughput screening methods performed on-site. The demand for point-of-care testing, able to distinguish molecular signatures with high accuracy, sensitivity and specificity has been significantly increasing. This new requirement relies on the on-site detection and monitorization of molecular signatures suitable for the surveillance of food production and processing. The widespread use of antibiotics has contributed to disease control of livestock but has also created problems for the dairy industry and consumers. Its therapeutic and subtherapeutic use has increased the risk of contamination in milk in enough concentrations to cause economic losses to the dairy industry and have a health impact in highly sensitive individuals. This study focuses on the development of a simple Surface-Enhanced Raman Spectroscopy (SERS) method for fast high throughput screening of tetracycline (TET) in milk. For this, we integrate a paper-based low-cost, fully recyclable and highly stable SERS platform, with a minimal sample preparation protocol. A two-microliter sample of milk solutions spiked with TET (from 0.01 to 1000 ppm) is dried on a silver nanoparticle coated cardboard substrate and measured via a Raman spectrophotometer. The SERS substrate showed to be extremely stable with a shelf life of several months. A global spectrum principal component analysis approach was used to test all the detected vibrational modes and their correlation with TET concentration. Peak intensity ratios (455 cm−1/1280 cm−1 and 874 cm−1/1397 cm−1) were found to be correlated with TET concentrations in milk, achieving a sensitivity as low as 0.1 ppm. Results indicate that this SERS method combined with portable Raman spectrometer is a potential tool that can be used on-site for the monitoring of TET residues and other antibiotics.CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)DCM - Departamento de Ciência dos MateriaisUCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNMarques, AnaVeigas, BrunoAraújo, AndreiaPagará, BeatrizBaptista, Pedro VianaÁguas, HugoMartins, RodrigoFortunato, Elvira2023-01-04T22:14:16Z2019-12-012019-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/146963eng2045-2322PURE: 17189406https://doi.org/10.1038/s41598-019-54380-yinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:27:56Zoai:run.unl.pt:10362/146963Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:52:43.424660Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk |
title |
Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk |
spellingShingle |
Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk Marques, Ana General |
title_short |
Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk |
title_full |
Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk |
title_fullStr |
Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk |
title_full_unstemmed |
Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk |
title_sort |
Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk |
author |
Marques, Ana |
author_facet |
Marques, Ana Veigas, Bruno Araújo, Andreia Pagará, Beatriz Baptista, Pedro Viana Águas, Hugo Martins, Rodrigo Fortunato, Elvira |
author_role |
author |
author2 |
Veigas, Bruno Araújo, Andreia Pagará, Beatriz Baptista, Pedro Viana Águas, Hugo Martins, Rodrigo Fortunato, Elvira |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) DCM - Departamento de Ciência dos Materiais UCIBIO - Applied Molecular Biosciences Unit DCV - Departamento de Ciências da Vida RUN |
dc.contributor.author.fl_str_mv |
Marques, Ana Veigas, Bruno Araújo, Andreia Pagará, Beatriz Baptista, Pedro Viana Águas, Hugo Martins, Rodrigo Fortunato, Elvira |
dc.subject.por.fl_str_mv |
General |
topic |
General |
description |
PTDC/CTM-NAN/2912/2014. PTDC/AAG-TEC/5400/2014. POCI-01-0145-FEDER-016637. POCI-01-0145-FEDER-007688. UID/CTM/50025/2019. FCT/MCTES SFRH/BD/115173/2016 and SFRH/BPD/124311/2016 . |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-01 2019-12-01T00:00:00Z 2023-01-04T22:14:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/146963 |
url |
http://hdl.handle.net/10362/146963 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2045-2322 PURE: 17189406 https://doi.org/10.1038/s41598-019-54380-y |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799138118343852032 |