Substrate Topography Modulates Cell Aging on a Progeria Cell Model
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/92391 https://doi.org/10.1021/acsbiomaterials.8b00224 |
Resumo: | Aging is characterized by a progressive accumulation of cellular damage, which leads to impaired function. Little is known whether substrates can influence cell aging. This is of utmost importance in the development of medical devices that are in contact with human tissue for long periods of time. To address this question, we have used an accelerated aging cell model derived from Hutchinson-Gilford Progeria Syndrome (HGPS) induced pluripotent stem cells (iPSCs). Our results show that HGPS-iPSC smooth muscle cells (SMCs) have an increased aging profile in substrates with specific micropatterns than in flat ones. This is characterized by an up-regulation in the expression of progerin, β-galactosidase, annexin 3 and 5, and caspase 9. Signs of cell aging are also observed in SMCs without HGPS cultured in substrates with specific microtopographies. It is further showed that specific micropatterned substrates induce cell aging by triggering a DNA damage program likely by the disruption between cyto- and nucleoskeleton. |
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Substrate Topography Modulates Cell Aging on a Progeria Cell ModelAging is characterized by a progressive accumulation of cellular damage, which leads to impaired function. Little is known whether substrates can influence cell aging. This is of utmost importance in the development of medical devices that are in contact with human tissue for long periods of time. To address this question, we have used an accelerated aging cell model derived from Hutchinson-Gilford Progeria Syndrome (HGPS) induced pluripotent stem cells (iPSCs). Our results show that HGPS-iPSC smooth muscle cells (SMCs) have an increased aging profile in substrates with specific micropatterns than in flat ones. This is characterized by an up-regulation in the expression of progerin, β-galactosidase, annexin 3 and 5, and caspase 9. Signs of cell aging are also observed in SMCs without HGPS cultured in substrates with specific microtopographies. It is further showed that specific micropatterned substrates induce cell aging by triggering a DNA damage program likely by the disruption between cyto- and nucleoskeleton.American Chemical Society2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/92391http://hdl.handle.net/10316/92391https://doi.org/10.1021/acsbiomaterials.8b00224eng2373-98782373-9878Pitrez, Patrícia R.Estronca, Luís Miguel Beicinha BrancoVazão, HelenaEgesipe, Anne-LaureLe Corf, AmélieNavarro, ClaireLévy, NicolasDe Sandre-Giovannoli, AnnachiaraNissan, XavierFerreira, Linoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-05-25T05:49:29Zoai:estudogeral.uc.pt:10316/92391Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:11:29.934434Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Substrate Topography Modulates Cell Aging on a Progeria Cell Model |
title |
Substrate Topography Modulates Cell Aging on a Progeria Cell Model |
spellingShingle |
Substrate Topography Modulates Cell Aging on a Progeria Cell Model Pitrez, Patrícia R. |
title_short |
Substrate Topography Modulates Cell Aging on a Progeria Cell Model |
title_full |
Substrate Topography Modulates Cell Aging on a Progeria Cell Model |
title_fullStr |
Substrate Topography Modulates Cell Aging on a Progeria Cell Model |
title_full_unstemmed |
Substrate Topography Modulates Cell Aging on a Progeria Cell Model |
title_sort |
Substrate Topography Modulates Cell Aging on a Progeria Cell Model |
author |
Pitrez, Patrícia R. |
author_facet |
Pitrez, Patrícia R. Estronca, Luís Miguel Beicinha Branco Vazão, Helena Egesipe, Anne-Laure Le Corf, Amélie Navarro, Claire Lévy, Nicolas De Sandre-Giovannoli, Annachiara Nissan, Xavier Ferreira, Lino |
author_role |
author |
author2 |
Estronca, Luís Miguel Beicinha Branco Vazão, Helena Egesipe, Anne-Laure Le Corf, Amélie Navarro, Claire Lévy, Nicolas De Sandre-Giovannoli, Annachiara Nissan, Xavier Ferreira, Lino |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Pitrez, Patrícia R. Estronca, Luís Miguel Beicinha Branco Vazão, Helena Egesipe, Anne-Laure Le Corf, Amélie Navarro, Claire Lévy, Nicolas De Sandre-Giovannoli, Annachiara Nissan, Xavier Ferreira, Lino |
description |
Aging is characterized by a progressive accumulation of cellular damage, which leads to impaired function. Little is known whether substrates can influence cell aging. This is of utmost importance in the development of medical devices that are in contact with human tissue for long periods of time. To address this question, we have used an accelerated aging cell model derived from Hutchinson-Gilford Progeria Syndrome (HGPS) induced pluripotent stem cells (iPSCs). Our results show that HGPS-iPSC smooth muscle cells (SMCs) have an increased aging profile in substrates with specific micropatterns than in flat ones. This is characterized by an up-regulation in the expression of progerin, β-galactosidase, annexin 3 and 5, and caspase 9. Signs of cell aging are also observed in SMCs without HGPS cultured in substrates with specific microtopographies. It is further showed that specific micropatterned substrates induce cell aging by triggering a DNA damage program likely by the disruption between cyto- and nucleoskeleton. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/92391 http://hdl.handle.net/10316/92391 https://doi.org/10.1021/acsbiomaterials.8b00224 |
url |
http://hdl.handle.net/10316/92391 https://doi.org/10.1021/acsbiomaterials.8b00224 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2373-9878 2373-9878 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134012047884288 |