Imprinting fidelity in mouse iPSCs depends on sex of donor cell and medium formulation

Detalhes bibliográficos
Autor(a) principal: Arez, Maria
Data de Publicação: 2022
Outros Autores: Eckersley-Maslin, Melanie, Klobuar, Tajda, von Gilsa Lopes, João, Krueger, Felix, Mupo, Annalisa, Raposo, Ana Cláudia, Oxley, David, Mancino, Samantha, Gendrel, Anne-Valerie, Jesus, Bruno Bernardes De, da Rocha, Simão T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/54505
Resumo: © The Author(s) 2022 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
id RCAP_9c19cf29fa8422709d631a1b874670b6
oai_identifier_str oai:repositorio.ul.pt:10451/54505
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Imprinting fidelity in mouse iPSCs depends on sex of donor cell and medium formulation© The Author(s) 2022 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.Reprogramming of somatic cells into induced Pluripotent Stem Cells (iPSCs) is a major leap towards personalised approaches to disease modelling and cell-replacement therapies. However, we still lack the ability to fully control the epigenetic status of iPSCs, which is a major hurdle for their downstream applications. Epigenetic fidelity can be tracked by genomic imprinting, a phenomenon dependent on DNA methylation, which is frequently perturbed in iPSCs by yet unknown reasons. To try to understand the causes underlying these defects, we conducted a thorough imprinting analysis using IMPLICON, a high-throughput method measuring DNA methylation levels, in multiple female and male murine iPSC lines generated under different experimental conditions. Our results show that imprinting defects are remarkably common in iPSCs, but their nature depends on the sex of donor cells and their response to culture conditions. Imprints in female iPSCs resist the initial genome-wide DNA demethylation wave during reprogramming, but ultimately cells accumulate hypomethylation defects irrespective of culture medium formulations. In contrast, imprinting defects on male iPSCs depends on the experimental conditions and arise during reprogramming, being mitigated by the addition of vitamin C (VitC). Our findings are fundamental to further optimise reprogramming strategies and generate iPSCs with a stable epigenome.Work in S.T.d.R.’s team was supported by Fundação para a Ciência e Tecnologia (FCT) Ministério da Ciência, Tecnologia e Ensino Superior (MCTES), Portugal [IC&DT projects PTDC/BEX-BCM/2612/2014 and PTDC/BIA-MOL/29320/2017 as well as projects UIDB/04565/2020 and UIDP/04565/2020 of the Research Unit Institute from Bioengineering and Biosciences – iBB and LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy – i4HB]; S.T.d.R. and A.-V.G. are supported by assistant research contracts from FCT/MCTES (CEECIND/01234/2017 and CEECIND/02085/2018, respectively); M.A and A.C.R. are supported, respectively, by SFRH/BD/151251/2021 and SFRH/BD/137099/2018 PhD fellowships from FCT/MCTES. J.V.G.L is supported by COVID/BD/152624/2022 from FCT/MCTES. MAE-M was supported by a BBSRC Discovery Fellowship (BB/T009713/1) and is now supported by a Snow Medical Fellowship. F.K. is supported by the Babraham Institute Strategic Core Funding and A.M. by BBSRC BBS/E/B/000C0421. B.B.J. work was funded by Fundação para a Ciência e Tecnologia (FCT), and FEDER, LISBOA-01-0145-FEDER-028534, project co-funded by FEDER, through POR Lisboa 2020—Programa Operacional Regional de Lisboa. T.K. is supported by Janko Jamnik Doctoral Scholarship from National Institute of Chemistry.Springer NatureRepositório da Universidade de LisboaArez, MariaEckersley-Maslin, MelanieKlobuar, Tajdavon Gilsa Lopes, JoãoKrueger, FelixMupo, AnnalisaRaposo, Ana CláudiaOxley, DavidMancino, SamanthaGendrel, Anne-ValerieJesus, Bruno Bernardes Deda Rocha, Simão T.2022-09-19T15:14:17Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/54505engNat Commun. 2022 Sep 16;13(1):543210.1038/s41467-022-33013-52041-1723info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T17:00:55Zoai:repositorio.ul.pt:10451/54505Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:05:19.449285Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Imprinting fidelity in mouse iPSCs depends on sex of donor cell and medium formulation
title Imprinting fidelity in mouse iPSCs depends on sex of donor cell and medium formulation
spellingShingle Imprinting fidelity in mouse iPSCs depends on sex of donor cell and medium formulation
Arez, Maria
title_short Imprinting fidelity in mouse iPSCs depends on sex of donor cell and medium formulation
title_full Imprinting fidelity in mouse iPSCs depends on sex of donor cell and medium formulation
title_fullStr Imprinting fidelity in mouse iPSCs depends on sex of donor cell and medium formulation
title_full_unstemmed Imprinting fidelity in mouse iPSCs depends on sex of donor cell and medium formulation
title_sort Imprinting fidelity in mouse iPSCs depends on sex of donor cell and medium formulation
author Arez, Maria
author_facet Arez, Maria
Eckersley-Maslin, Melanie
Klobuar, Tajda
von Gilsa Lopes, João
Krueger, Felix
Mupo, Annalisa
Raposo, Ana Cláudia
Oxley, David
Mancino, Samantha
Gendrel, Anne-Valerie
Jesus, Bruno Bernardes De
da Rocha, Simão T.
author_role author
author2 Eckersley-Maslin, Melanie
Klobuar, Tajda
von Gilsa Lopes, João
Krueger, Felix
Mupo, Annalisa
Raposo, Ana Cláudia
Oxley, David
Mancino, Samantha
Gendrel, Anne-Valerie
Jesus, Bruno Bernardes De
da Rocha, Simão T.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Arez, Maria
Eckersley-Maslin, Melanie
Klobuar, Tajda
von Gilsa Lopes, João
Krueger, Felix
Mupo, Annalisa
Raposo, Ana Cláudia
Oxley, David
Mancino, Samantha
Gendrel, Anne-Valerie
Jesus, Bruno Bernardes De
da Rocha, Simão T.
description © The Author(s) 2022 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-19T15:14:17Z
2022
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/54505
url http://hdl.handle.net/10451/54505
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nat Commun. 2022 Sep 16;13(1):5432
10.1038/s41467-022-33013-5
2041-1723
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799134604468158464