Fucoxanthin’s optimization from undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studies

Detalhes bibliográficos
Autor(a) principal: Lourenço-Lopes, Catarina
Data de Publicação: 2010
Outros Autores: Fraga-Corral, Maria, Soria-Lopez, Anton, Núñez Estévez, Bernabé, Barral Martínez, Marta, Silva, Aurora, Li, Ningyang, Liu, Chao, Simal-Gandara, Jesus, Prieto Lage, Miguel A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/1977
Resumo: Brown macroalgae are a potential source of natural pigments. Among them, Undaria pinnatifida is recognized for its high concentration of fucoxanthin (Fx), which is a pigment with a wide range of bioactivities. In this study, three independent parameters were optimized for conventional heat extraction (CHE) to maximize the recovery of Fx from Undaria pinnatifida. Optimal conditions (temperature = 45 °C, solvent = 70%, and time = 61 min) extracted 5.1 mg Fx/g dw. Later, the bioactivities of the Fx-rich extracts (antioxidant, antimicrobial, and neuroprotective) were assessed using in vitro and in silico approaches. In vitro assays indicated that Fx has a strong antioxidant capacity and even stronger antimicrobial activity against gram-positive bacteria. This data was supported in silico where Fx established a high binding affinity to DR, a Staphylococcus aureus protein, through aa ALA-8, LEU-21, and other alkane interactions. Finally, the in vitro enzymatic inhibition of AChE using Fx, was further supported using docking models that displayed Fx as having a high affinity for aa TYR72 and THR 75; therefore, the Fx extraction behavior explored in this work may reduce the costs associated with energy and solvent consumption. Moreover, this paper demonstrates the efficiency of CHE when recovering high amounts of Fx from Undaria pinnatifida. Furthermore, these findings can be applied in different industries.
id RCAP_9f160520db139303ecc29e70976636f7
oai_identifier_str oai:bibliotecadigital.ipb.pt:10198/1977
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Fucoxanthin’s optimization from undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studiesFucoxanthinConventional heat extractionUndaria pinnatifidaKineticsAntioxidant activityNeuroprotective activityAntimicrobial activityIn silico studiesDockingBrown macroalgae are a potential source of natural pigments. Among them, Undaria pinnatifida is recognized for its high concentration of fucoxanthin (Fx), which is a pigment with a wide range of bioactivities. In this study, three independent parameters were optimized for conventional heat extraction (CHE) to maximize the recovery of Fx from Undaria pinnatifida. Optimal conditions (temperature = 45 °C, solvent = 70%, and time = 61 min) extracted 5.1 mg Fx/g dw. Later, the bioactivities of the Fx-rich extracts (antioxidant, antimicrobial, and neuroprotective) were assessed using in vitro and in silico approaches. In vitro assays indicated that Fx has a strong antioxidant capacity and even stronger antimicrobial activity against gram-positive bacteria. This data was supported in silico where Fx established a high binding affinity to DR, a Staphylococcus aureus protein, through aa ALA-8, LEU-21, and other alkane interactions. Finally, the in vitro enzymatic inhibition of AChE using Fx, was further supported using docking models that displayed Fx as having a high affinity for aa TYR72 and THR 75; therefore, the Fx extraction behavior explored in this work may reduce the costs associated with energy and solvent consumption. Moreover, this paper demonstrates the efficiency of CHE when recovering high amounts of Fx from Undaria pinnatifida. Furthermore, these findings can be applied in different industries.MDPIBiblioteca Digital do IPBLourenço-Lopes, CatarinaFraga-Corral, MariaSoria-Lopez, AntonNúñez Estévez, BernabéBarral Martínez, MartaSilva, AuroraLi, NingyangLiu, ChaoSimal-Gandara, JesusPrieto Lage, Miguel A.2010-02-17T17:57:17Z20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/1977engLourenço-Lopes, Catarina; Fraga-Corral, Maria; Soria-Lopez, Anton; Núñez Estévez, Bernabé; Barral-Martinez, Marta; Silva, Aurora; Li, Ningyang; Liu, Chao; Simal-Gandara, Jesus; Prieto, Miguel A. (2022). Fucoxanthin’s optimization from undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studies. Antioxidants. EISSN 2076-3921. 11:7, p. 1-252076-3921info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-10-23T01:20:09Zoai:bibliotecadigital.ipb.pt:10198/1977Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-10-23T01:20:09Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Fucoxanthin’s optimization from undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studies
title Fucoxanthin’s optimization from undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studies
spellingShingle Fucoxanthin’s optimization from undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studies
Lourenço-Lopes, Catarina
Fucoxanthin
Conventional heat extraction
Undaria pinnatifida
Kinetics
Antioxidant activity
Neuroprotective activity
Antimicrobial activity
In silico studies
Docking
title_short Fucoxanthin’s optimization from undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studies
title_full Fucoxanthin’s optimization from undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studies
title_fullStr Fucoxanthin’s optimization from undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studies
title_full_unstemmed Fucoxanthin’s optimization from undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studies
title_sort Fucoxanthin’s optimization from undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studies
author Lourenço-Lopes, Catarina
author_facet Lourenço-Lopes, Catarina
Fraga-Corral, Maria
Soria-Lopez, Anton
Núñez Estévez, Bernabé
Barral Martínez, Marta
Silva, Aurora
Li, Ningyang
Liu, Chao
Simal-Gandara, Jesus
Prieto Lage, Miguel A.
author_role author
author2 Fraga-Corral, Maria
Soria-Lopez, Anton
Núñez Estévez, Bernabé
Barral Martínez, Marta
Silva, Aurora
Li, Ningyang
Liu, Chao
Simal-Gandara, Jesus
Prieto Lage, Miguel A.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Lourenço-Lopes, Catarina
Fraga-Corral, Maria
Soria-Lopez, Anton
Núñez Estévez, Bernabé
Barral Martínez, Marta
Silva, Aurora
Li, Ningyang
Liu, Chao
Simal-Gandara, Jesus
Prieto Lage, Miguel A.
dc.subject.por.fl_str_mv Fucoxanthin
Conventional heat extraction
Undaria pinnatifida
Kinetics
Antioxidant activity
Neuroprotective activity
Antimicrobial activity
In silico studies
Docking
topic Fucoxanthin
Conventional heat extraction
Undaria pinnatifida
Kinetics
Antioxidant activity
Neuroprotective activity
Antimicrobial activity
In silico studies
Docking
description Brown macroalgae are a potential source of natural pigments. Among them, Undaria pinnatifida is recognized for its high concentration of fucoxanthin (Fx), which is a pigment with a wide range of bioactivities. In this study, three independent parameters were optimized for conventional heat extraction (CHE) to maximize the recovery of Fx from Undaria pinnatifida. Optimal conditions (temperature = 45 °C, solvent = 70%, and time = 61 min) extracted 5.1 mg Fx/g dw. Later, the bioactivities of the Fx-rich extracts (antioxidant, antimicrobial, and neuroprotective) were assessed using in vitro and in silico approaches. In vitro assays indicated that Fx has a strong antioxidant capacity and even stronger antimicrobial activity against gram-positive bacteria. This data was supported in silico where Fx established a high binding affinity to DR, a Staphylococcus aureus protein, through aa ALA-8, LEU-21, and other alkane interactions. Finally, the in vitro enzymatic inhibition of AChE using Fx, was further supported using docking models that displayed Fx as having a high affinity for aa TYR72 and THR 75; therefore, the Fx extraction behavior explored in this work may reduce the costs associated with energy and solvent consumption. Moreover, this paper demonstrates the efficiency of CHE when recovering high amounts of Fx from Undaria pinnatifida. Furthermore, these findings can be applied in different industries.
publishDate 2010
dc.date.none.fl_str_mv 2010-02-17T17:57:17Z
2022
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/1977
url http://hdl.handle.net/10198/1977
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lourenço-Lopes, Catarina; Fraga-Corral, Maria; Soria-Lopez, Anton; Núñez Estévez, Bernabé; Barral-Martinez, Marta; Silva, Aurora; Li, Ningyang; Liu, Chao; Simal-Gandara, Jesus; Prieto, Miguel A. (2022). Fucoxanthin’s optimization from undaria pinnatifida using conventional heat extraction, bioactivity assays and in silico studies. Antioxidants. EISSN 2076-3921. 11:7, p. 1-25
2076-3921
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817543032578244608