Determination of Tetracycline Antibiotic Residues in Edible Swine Tissues by Liquid Chromatography with Spectrofluorometric Detection and Confirmation by Mass Spectrometry
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/10604 https://doi.org/10.1021/jf070398j |
Resumo: | A sensitive and specific method is described for the simultaneous determination of oxytetracycline, tetracycline (TC), and chlortetracycline residues in edible swine tissues, by combining liquid chromatography with spectrofluorometric and mass spectrometry detection. The procedure involved a preliminary extraction with EDTA-McIlvaine buffer acidified at pH 4.0, followed by solid-phase extraction cleanup using a polymeric sorbent. The liquid chromatography analysis was performed with spectrofluorometric detection after postcolumn derivatization with magnesium ions. The limits of quantification were 50 μg/kg for muscle and 100 μg/kg for kidney tissues. The recovery values were greater than 77.8% for muscle and 65.1% for kidney. The method has been successfully used for the quantification of tetracyclines in swine tissues samples. The selective liquid chromatography mass spectrometric analysis for confirmation of oxytetracycline in one positive swine muscle sample was made by atmospheric pressure chemical ionization (APCI). The APCI mass spectra of the TCs gave the protonated molecular ion and two typical fragment ions, required for their confirmation in single ion monitoring scan mode in animal tissues. |
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Determination of Tetracycline Antibiotic Residues in Edible Swine Tissues by Liquid Chromatography with Spectrofluorometric Detection and Confirmation by Mass SpectrometryTetracyclinesSwine tissuesLiquid chromatographySpectrofluorometric detectionMass spectrometric detectionA sensitive and specific method is described for the simultaneous determination of oxytetracycline, tetracycline (TC), and chlortetracycline residues in edible swine tissues, by combining liquid chromatography with spectrofluorometric and mass spectrometry detection. The procedure involved a preliminary extraction with EDTA-McIlvaine buffer acidified at pH 4.0, followed by solid-phase extraction cleanup using a polymeric sorbent. The liquid chromatography analysis was performed with spectrofluorometric detection after postcolumn derivatization with magnesium ions. The limits of quantification were 50 μg/kg for muscle and 100 μg/kg for kidney tissues. The recovery values were greater than 77.8% for muscle and 65.1% for kidney. The method has been successfully used for the quantification of tetracyclines in swine tissues samples. The selective liquid chromatography mass spectrometric analysis for confirmation of oxytetracycline in one positive swine muscle sample was made by atmospheric pressure chemical ionization (APCI). The APCI mass spectra of the TCs gave the protonated molecular ion and two typical fragment ions, required for their confirmation in single ion monitoring scan mode in animal tissues.Fundação para a Ciência e Tecnologia; POCTI/FEDERAmerican Chemical Society2007-06-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/10604http://hdl.handle.net/10316/10604https://doi.org/10.1021/jf070398jengJournal of Agricultural and Food Chemistry. 55:13 (2007) 4973-49790021-8561Pena, AngelinaLino, Celeste M.Alonso, RosaBarceló, Damiainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-09-20T08:12:35Zoai:estudogeral.uc.pt:10316/10604Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:47:23.409714Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Determination of Tetracycline Antibiotic Residues in Edible Swine Tissues by Liquid Chromatography with Spectrofluorometric Detection and Confirmation by Mass Spectrometry |
title |
Determination of Tetracycline Antibiotic Residues in Edible Swine Tissues by Liquid Chromatography with Spectrofluorometric Detection and Confirmation by Mass Spectrometry |
spellingShingle |
Determination of Tetracycline Antibiotic Residues in Edible Swine Tissues by Liquid Chromatography with Spectrofluorometric Detection and Confirmation by Mass Spectrometry Pena, Angelina Tetracyclines Swine tissues Liquid chromatography Spectrofluorometric detection Mass spectrometric detection |
title_short |
Determination of Tetracycline Antibiotic Residues in Edible Swine Tissues by Liquid Chromatography with Spectrofluorometric Detection and Confirmation by Mass Spectrometry |
title_full |
Determination of Tetracycline Antibiotic Residues in Edible Swine Tissues by Liquid Chromatography with Spectrofluorometric Detection and Confirmation by Mass Spectrometry |
title_fullStr |
Determination of Tetracycline Antibiotic Residues in Edible Swine Tissues by Liquid Chromatography with Spectrofluorometric Detection and Confirmation by Mass Spectrometry |
title_full_unstemmed |
Determination of Tetracycline Antibiotic Residues in Edible Swine Tissues by Liquid Chromatography with Spectrofluorometric Detection and Confirmation by Mass Spectrometry |
title_sort |
Determination of Tetracycline Antibiotic Residues in Edible Swine Tissues by Liquid Chromatography with Spectrofluorometric Detection and Confirmation by Mass Spectrometry |
author |
Pena, Angelina |
author_facet |
Pena, Angelina Lino, Celeste M. Alonso, Rosa Barceló, Damia |
author_role |
author |
author2 |
Lino, Celeste M. Alonso, Rosa Barceló, Damia |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Pena, Angelina Lino, Celeste M. Alonso, Rosa Barceló, Damia |
dc.subject.por.fl_str_mv |
Tetracyclines Swine tissues Liquid chromatography Spectrofluorometric detection Mass spectrometric detection |
topic |
Tetracyclines Swine tissues Liquid chromatography Spectrofluorometric detection Mass spectrometric detection |
description |
A sensitive and specific method is described for the simultaneous determination of oxytetracycline, tetracycline (TC), and chlortetracycline residues in edible swine tissues, by combining liquid chromatography with spectrofluorometric and mass spectrometry detection. The procedure involved a preliminary extraction with EDTA-McIlvaine buffer acidified at pH 4.0, followed by solid-phase extraction cleanup using a polymeric sorbent. The liquid chromatography analysis was performed with spectrofluorometric detection after postcolumn derivatization with magnesium ions. The limits of quantification were 50 μg/kg for muscle and 100 μg/kg for kidney tissues. The recovery values were greater than 77.8% for muscle and 65.1% for kidney. The method has been successfully used for the quantification of tetracyclines in swine tissues samples. The selective liquid chromatography mass spectrometric analysis for confirmation of oxytetracycline in one positive swine muscle sample was made by atmospheric pressure chemical ionization (APCI). The APCI mass spectra of the TCs gave the protonated molecular ion and two typical fragment ions, required for their confirmation in single ion monitoring scan mode in animal tissues. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-06-27 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/10604 http://hdl.handle.net/10316/10604 https://doi.org/10.1021/jf070398j |
url |
http://hdl.handle.net/10316/10604 https://doi.org/10.1021/jf070398j |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Agricultural and Food Chemistry. 55:13 (2007) 4973-4979 0021-8561 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799133751138058240 |