Determination of tetracycline and its major degradation products by liquid chromatography with fluorescence detection
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/5822 https://doi.org/10.1016/S0003-2670(99)00668-6 |
Resumo: | A liquid chromatographic method of tetracycline and its major degradation products on a C8-reversed phase column with acidic mobile phase and fluorescence detection is described. The quantification limit, measured as the amount of sample that gave a signal ten times the peak-to-peak noise of the baseline, was: 0.25 ng for tetracycline (TC) and epitetracycline (ETC), 25 ng for and 4-epianhydrotetracycline (EATC) and 50 ng for anhydrotetracycline (ATC) of injected standard. By means of this liquid chromatography (LC) assay TC, ETC, EATC and ATC as main degradation products of tetracycline, can be separated and determined with good sensitivity and specificity within 15 min. |
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Determination of tetracycline and its major degradation products by liquid chromatography with fluorescence detectionTetracyclineEpitetracyclineEpianydrotetracyclineAnydrotetracyclineLiquid chromatographyFluorescence detectionA liquid chromatographic method of tetracycline and its major degradation products on a C8-reversed phase column with acidic mobile phase and fluorescence detection is described. The quantification limit, measured as the amount of sample that gave a signal ten times the peak-to-peak noise of the baseline, was: 0.25 ng for tetracycline (TC) and epitetracycline (ETC), 25 ng for and 4-epianhydrotetracycline (EATC) and 50 ng for anhydrotetracycline (ATC) of injected standard. By means of this liquid chromatography (LC) assay TC, ETC, EATC and ATC as main degradation products of tetracycline, can be separated and determined with good sensitivity and specificity within 15 min.http://www.sciencedirect.com/science/article/B6TGX-43HVHWC-1D/1/9763379e028400de01242a673bd4528a1998info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/5822http://hdl.handle.net/10316/5822https://doi.org/10.1016/S0003-2670(99)00668-6engJournal of Pharmaceutical and Biomedical Analysis. 18:4-5 (1998) 839-845Pena, A.Carmona, A.Barbosa, A.Lino, C.Silveira, I.Castillo, B.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-10-28T10:13:50Zoai:estudogeral.uc.pt:10316/5822Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:47:19.227795Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Determination of tetracycline and its major degradation products by liquid chromatography with fluorescence detection |
title |
Determination of tetracycline and its major degradation products by liquid chromatography with fluorescence detection |
spellingShingle |
Determination of tetracycline and its major degradation products by liquid chromatography with fluorescence detection Pena, A. Tetracycline Epitetracycline Epianydrotetracycline Anydrotetracycline Liquid chromatography Fluorescence detection |
title_short |
Determination of tetracycline and its major degradation products by liquid chromatography with fluorescence detection |
title_full |
Determination of tetracycline and its major degradation products by liquid chromatography with fluorescence detection |
title_fullStr |
Determination of tetracycline and its major degradation products by liquid chromatography with fluorescence detection |
title_full_unstemmed |
Determination of tetracycline and its major degradation products by liquid chromatography with fluorescence detection |
title_sort |
Determination of tetracycline and its major degradation products by liquid chromatography with fluorescence detection |
author |
Pena, A. |
author_facet |
Pena, A. Carmona, A. Barbosa, A. Lino, C. Silveira, I. Castillo, B. |
author_role |
author |
author2 |
Carmona, A. Barbosa, A. Lino, C. Silveira, I. Castillo, B. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pena, A. Carmona, A. Barbosa, A. Lino, C. Silveira, I. Castillo, B. |
dc.subject.por.fl_str_mv |
Tetracycline Epitetracycline Epianydrotetracycline Anydrotetracycline Liquid chromatography Fluorescence detection |
topic |
Tetracycline Epitetracycline Epianydrotetracycline Anydrotetracycline Liquid chromatography Fluorescence detection |
description |
A liquid chromatographic method of tetracycline and its major degradation products on a C8-reversed phase column with acidic mobile phase and fluorescence detection is described. The quantification limit, measured as the amount of sample that gave a signal ten times the peak-to-peak noise of the baseline, was: 0.25 ng for tetracycline (TC) and epitetracycline (ETC), 25 ng for and 4-epianhydrotetracycline (EATC) and 50 ng for anhydrotetracycline (ATC) of injected standard. By means of this liquid chromatography (LC) assay TC, ETC, EATC and ATC as main degradation products of tetracycline, can be separated and determined with good sensitivity and specificity within 15 min. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/5822 http://hdl.handle.net/10316/5822 https://doi.org/10.1016/S0003-2670(99)00668-6 |
url |
http://hdl.handle.net/10316/5822 https://doi.org/10.1016/S0003-2670(99)00668-6 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Pharmaceutical and Biomedical Analysis. 18:4-5 (1998) 839-845 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
aplication/PDF |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133750567632896 |