PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applications
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/11110/611 |
Resumo: | Fiber meshes of poly(hydroxybutyrate) (PHB) and poly(hydroxybutyrate)/ poly(ethylene oxide) (PHB/PEO) with different concentrations of chlorhexidine (CHX) were prepared by electrospinning, for assessment as a polymer based drug delivery system. The electrospun fibers were characterized at morphological, molecular and mechanical levels. The bactericidal potential of PHB and PHB/PEO electrospun fibers with and without CHX was investigated against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by disk diffusion susceptibility tests. Electrospun fibers containing CHX exhibited bactericidal activity. PHB/PEO-1%CHX displayed higher CHX release levels and equivalent antibacterial activity when compared to PHB/PEO with 5 and 10 wt% CHX. Bactericidal performance of samples with 1 wt% CHX was assessed by Colony Forming Units (CFU), where a reduction of 100 % and 99.69 % against E. coli and S. aureus were achieved, respectively. |
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PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applicationsElectrospun fibersDrug release kineticsAntibacterial activityBiopolymersChlorhexidineFiber meshes of poly(hydroxybutyrate) (PHB) and poly(hydroxybutyrate)/ poly(ethylene oxide) (PHB/PEO) with different concentrations of chlorhexidine (CHX) were prepared by electrospinning, for assessment as a polymer based drug delivery system. The electrospun fibers were characterized at morphological, molecular and mechanical levels. The bactericidal potential of PHB and PHB/PEO electrospun fibers with and without CHX was investigated against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by disk diffusion susceptibility tests. Electrospun fibers containing CHX exhibited bactericidal activity. PHB/PEO-1%CHX displayed higher CHX release levels and equivalent antibacterial activity when compared to PHB/PEO with 5 and 10 wt% CHX. Bactericidal performance of samples with 1 wt% CHX was assessed by Colony Forming Units (CFU), where a reduction of 100 % and 99.69 % against E. coli and S. aureus were achieved, respectively.This work was supported by FEDER through the COMPETE Program and by the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Project PEST-C/FIS/UI607/2011 and PEST-C/QUI/UIO686/2011. The authors also thank funding from Matepro –Optimizing Materials and Processes”, ref. NORTE-07-0124-FEDER-000037”, co-funded by the “Programa Operacional Regional do Norte” (ON.2 – O Novo Norte), under the “Quadro de Referência Estratégico Nacional” (QREN), through the “Fundo Europeu de Desenvolvimento Regional” (FEDER). D.M.C, JP and VS thanks the FCT for the, SFRH/BD/ 82411/2011, SFRH/BD/64901/2009 and SFRH/BPD/63148/2009 grants respectively. The authors also thank support from the COST Action MP1003, 2010 ‘European Scientific Network for Artificial Muscles’ and to the COST Action MP1206 ‘Electrospun Nano-fibres for Bio inspired Composite Materials and Innovative Industrial Applications’. The authors also thank prof. José Luis Gomez Ribelles from the Unversidad Politécnica de Valencia, Spain, for interesting discussions on these issues.2014-02-04T19:05:46Z2014-02-04T19:05:46Z2013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/11110/611oai:ciencipca.ipca.pt:11110/611eng0142-9418http://hdl.handle.net/11110/611Fernandes, J. G.Correia, D. M.Botelho, G.Padrão, J.Ribeiro, C.Lanceros-Mendez, S.Sencadas, V.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-05T12:52:11Zoai:ciencipca.ipca.pt:11110/611Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:01:04.434868Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applications |
title |
PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applications |
spellingShingle |
PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applications Fernandes, J. G. Electrospun fibers Drug release kinetics Antibacterial activity Biopolymers Chlorhexidine |
title_short |
PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applications |
title_full |
PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applications |
title_fullStr |
PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applications |
title_full_unstemmed |
PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applications |
title_sort |
PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applications |
author |
Fernandes, J. G. |
author_facet |
Fernandes, J. G. Correia, D. M. Botelho, G. Padrão, J. Ribeiro, C. Lanceros-Mendez, S. Sencadas, V. |
author_role |
author |
author2 |
Correia, D. M. Botelho, G. Padrão, J. Ribeiro, C. Lanceros-Mendez, S. Sencadas, V. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Fernandes, J. G. Correia, D. M. Botelho, G. Padrão, J. Ribeiro, C. Lanceros-Mendez, S. Sencadas, V. |
dc.subject.por.fl_str_mv |
Electrospun fibers Drug release kinetics Antibacterial activity Biopolymers Chlorhexidine |
topic |
Electrospun fibers Drug release kinetics Antibacterial activity Biopolymers Chlorhexidine |
description |
Fiber meshes of poly(hydroxybutyrate) (PHB) and poly(hydroxybutyrate)/ poly(ethylene oxide) (PHB/PEO) with different concentrations of chlorhexidine (CHX) were prepared by electrospinning, for assessment as a polymer based drug delivery system. The electrospun fibers were characterized at morphological, molecular and mechanical levels. The bactericidal potential of PHB and PHB/PEO electrospun fibers with and without CHX was investigated against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by disk diffusion susceptibility tests. Electrospun fibers containing CHX exhibited bactericidal activity. PHB/PEO-1%CHX displayed higher CHX release levels and equivalent antibacterial activity when compared to PHB/PEO with 5 and 10 wt% CHX. Bactericidal performance of samples with 1 wt% CHX was assessed by Colony Forming Units (CFU), where a reduction of 100 % and 99.69 % against E. coli and S. aureus were achieved, respectively. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01T00:00:00Z 2014-02-04T19:05:46Z 2014-02-04T19:05:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/11110/611 oai:ciencipca.ipca.pt:11110/611 |
url |
http://hdl.handle.net/11110/611 |
identifier_str_mv |
oai:ciencipca.ipca.pt:11110/611 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0142-9418 http://hdl.handle.net/11110/611 |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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