Starch determination in Chlorella vulgaris-a comparison between acid and enzymatic methods

Detalhes bibliográficos
Autor(a) principal: Fernandes, Bruno Daniel
Data de Publicação: 2012
Outros Autores: Dragone, Giuliano, Abreu, Ana Paula, Geada, Pedro Miguel Macedo, Teixeira, J. A., Vicente, A. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/22461
Resumo: Different methods for estimating starch in Chlorella vulgaris were compared with the view of establishing a procedure suitable for rapid and accurate determination of starch content in this microalgal species. A close agreement was observed between methods that use perchloric acid and enzymatic methods that use α-amylase and amyloglucosidase to hydrolyze the starch of microalgae grown under different nitrogen culture conditions. Starch values obtained by these methods were significantly higher than those estimated by using hydrochloric acid as solubilizing and hydrolyzing agent. The enzymatic method (EM1) proved to be the most rapid and precise method for microalgal starch quantification. Furthermore, the evaluation of resistant starch by enzymatic methods assayed in nitrogen-sufficient and nitrogen-starved cells showed that no formation of this type of starch occurred in microalgae, meaning that this should not interfere with starch content determinations.
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spelling Starch determination in Chlorella vulgaris-a comparison between acid and enzymatic methodsAmylolytic enzymesBiofuelsChlorella vulgarisMicroalgaePerchloric acidStarch hydrolysisScience & TechnologyDifferent methods for estimating starch in Chlorella vulgaris were compared with the view of establishing a procedure suitable for rapid and accurate determination of starch content in this microalgal species. A close agreement was observed between methods that use perchloric acid and enzymatic methods that use α-amylase and amyloglucosidase to hydrolyze the starch of microalgae grown under different nitrogen culture conditions. Starch values obtained by these methods were significantly higher than those estimated by using hydrochloric acid as solubilizing and hydrolyzing agent. The enzymatic method (EM1) proved to be the most rapid and precise method for microalgal starch quantification. Furthermore, the evaluation of resistant starch by enzymatic methods assayed in nitrogen-sufficient and nitrogen-starved cells showed that no formation of this type of starch occurred in microalgae, meaning that this should not interfere with starch content determinations.This research work was supported by the grants SFRH/BD/44724/2008 (Bruno Fernandes) from Fundacao para a Ciencia e a Tecnologia (Portugal) and SFRH/BPD/44935/2008 (Giuliano Dragone). The authors also acknowledge the financial support received through the projects INNOVALGAE (FCT PTDC/AAC-AMB/108511/2008) and ALGANOL.KluwerUniversidade do MinhoFernandes, Bruno DanielDragone, GiulianoAbreu, Ana PaulaGeada, Pedro Miguel MacedoTeixeira, J. A.Vicente, A. A.20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/22461eng0921-897110.1007/s10811-011-9761-5info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:34:11Zoai:repositorium.sdum.uminho.pt:1822/22461Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:29:49.249076Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Starch determination in Chlorella vulgaris-a comparison between acid and enzymatic methods
title Starch determination in Chlorella vulgaris-a comparison between acid and enzymatic methods
spellingShingle Starch determination in Chlorella vulgaris-a comparison between acid and enzymatic methods
Fernandes, Bruno Daniel
Amylolytic enzymes
Biofuels
Chlorella vulgaris
Microalgae
Perchloric acid
Starch hydrolysis
Science & Technology
title_short Starch determination in Chlorella vulgaris-a comparison between acid and enzymatic methods
title_full Starch determination in Chlorella vulgaris-a comparison between acid and enzymatic methods
title_fullStr Starch determination in Chlorella vulgaris-a comparison between acid and enzymatic methods
title_full_unstemmed Starch determination in Chlorella vulgaris-a comparison between acid and enzymatic methods
title_sort Starch determination in Chlorella vulgaris-a comparison between acid and enzymatic methods
author Fernandes, Bruno Daniel
author_facet Fernandes, Bruno Daniel
Dragone, Giuliano
Abreu, Ana Paula
Geada, Pedro Miguel Macedo
Teixeira, J. A.
Vicente, A. A.
author_role author
author2 Dragone, Giuliano
Abreu, Ana Paula
Geada, Pedro Miguel Macedo
Teixeira, J. A.
Vicente, A. A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Fernandes, Bruno Daniel
Dragone, Giuliano
Abreu, Ana Paula
Geada, Pedro Miguel Macedo
Teixeira, J. A.
Vicente, A. A.
dc.subject.por.fl_str_mv Amylolytic enzymes
Biofuels
Chlorella vulgaris
Microalgae
Perchloric acid
Starch hydrolysis
Science & Technology
topic Amylolytic enzymes
Biofuels
Chlorella vulgaris
Microalgae
Perchloric acid
Starch hydrolysis
Science & Technology
description Different methods for estimating starch in Chlorella vulgaris were compared with the view of establishing a procedure suitable for rapid and accurate determination of starch content in this microalgal species. A close agreement was observed between methods that use perchloric acid and enzymatic methods that use α-amylase and amyloglucosidase to hydrolyze the starch of microalgae grown under different nitrogen culture conditions. Starch values obtained by these methods were significantly higher than those estimated by using hydrochloric acid as solubilizing and hydrolyzing agent. The enzymatic method (EM1) proved to be the most rapid and precise method for microalgal starch quantification. Furthermore, the evaluation of resistant starch by enzymatic methods assayed in nitrogen-sufficient and nitrogen-starved cells showed that no formation of this type of starch occurred in microalgae, meaning that this should not interfere with starch content determinations.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/22461
url http://hdl.handle.net/1822/22461
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0921-8971
10.1007/s10811-011-9761-5
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Kluwer
publisher.none.fl_str_mv Kluwer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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