Antimicrobial resistance determinants in Staphylococcus spp. recovered from birds of prey in Portugal
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/2956 |
Resumo: | Antibiotic resistance among wild animals represent an emerging public health concern. The objective of this study was to analyze the staphylococcal nasal microbiota in birds of prey and their content in antimicrobial resistance determinants. Nasal samples from 16 birds of prey were collected, swabs were dipped and incubated into BHI broth [6.5% NaCl] and later seeded on manitol salt agar and oxacillin-resistance screening agar base media. Staphylococcal colonies were isolated from both media and were identified by biochemical and molecular methods. Susceptibility testing to 18 antimicrobial agents was performed by disk-diffusion method. Six of the 16 tested animals carried staphylococci (37.5%) and 7 isolates of the following species were recovered: Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Staphylococcus sciuri rodentium, Staphylococcus cohnii urealitycum, and Staphylococcus gallinarum. The S. aureus isolate was penicillin-resistant (with blaZ gene) but methicillin-susceptible and was ascribed to spa-type t012, sequence-type ST30 and agr-type III. The S. epidermidis isolate carried blaZ, mecA, mrs(A/B), mphC, tet(K), drfA, and fusC genes, ica operon, and was typed as ST35. The genes ant6′-Ia, tet(K), tet(L), dfrG, cat221, cat194, and cat223 were detected in S. saprophyticus or S. gallinarum isolates. Birds of prey seem to be a natural reservoir of S. aureus and coagulase-negative staphylococci resistant to multiple antibiotics. Due to the convergence between habitats, the contact between wildlife, other animals and humans is now more common and this involves an increased possibility of interchange of these microorganisms in the different ecosystems. |
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Antimicrobial resistance determinants in Staphylococcus spp. recovered from birds of prey in PortugalResistência aos AntibióticosCoagulase-negative staphylococciBirds of PreyIca OperonS. aureusS. epidermidisPortugalAntibiotic resistance among wild animals represent an emerging public health concern. The objective of this study was to analyze the staphylococcal nasal microbiota in birds of prey and their content in antimicrobial resistance determinants. Nasal samples from 16 birds of prey were collected, swabs were dipped and incubated into BHI broth [6.5% NaCl] and later seeded on manitol salt agar and oxacillin-resistance screening agar base media. Staphylococcal colonies were isolated from both media and were identified by biochemical and molecular methods. Susceptibility testing to 18 antimicrobial agents was performed by disk-diffusion method. Six of the 16 tested animals carried staphylococci (37.5%) and 7 isolates of the following species were recovered: Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Staphylococcus sciuri rodentium, Staphylococcus cohnii urealitycum, and Staphylococcus gallinarum. The S. aureus isolate was penicillin-resistant (with blaZ gene) but methicillin-susceptible and was ascribed to spa-type t012, sequence-type ST30 and agr-type III. The S. epidermidis isolate carried blaZ, mecA, mrs(A/B), mphC, tet(K), drfA, and fusC genes, ica operon, and was typed as ST35. The genes ant6′-Ia, tet(K), tet(L), dfrG, cat221, cat194, and cat223 were detected in S. saprophyticus or S. gallinarum isolates. Birds of prey seem to be a natural reservoir of S. aureus and coagulase-negative staphylococci resistant to multiple antibiotics. Due to the convergence between habitats, the contact between wildlife, other animals and humans is now more common and this involves an increased possibility of interchange of these microorganisms in the different ecosystems.Margarida Andrade Sousa has her Ph.D. fellowship granted by FCT (Fundac¸a˜o para a Cieˆncia e Tecnologia, Portugal) with the reference SFRH/BD/87302/2012 co- financed by the Social European Union Found and the Operational Program for Human Potential and National Board for Reference and Strategic Programs (POPH/QREN), Portugal. Nuno Silva was supported by ‘‘Programa Cieˆncia 2008’’ co-financed by POPH/QREN Type 4.2: Employment Promotion Scientific subsidized by the European Social Fund and National Funds of the Ministry of Science and Technology for Higher Education (MCTES). Part of this work was supported by Project SAF2012- 35474 from the Ministerio de Economía y Competitividad of Spain and the Fondo Europeo de Desarrollo Regional (FEDER).ElsevierRepositório Científico do Instituto Nacional de SaúdeSousa, MargaridaSilva, NunoIgrejas, GilbertoSilva, FilipeSargo, RobertoAlegria, NunoBenito, DanielGómez, PaulaLozano, CarmenGómez-Sanz, ElenaTorres, CarmenCaniça, ManuelaPoeta, Patrícia2015-02-25T17:00:59Z2014-062014-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/2956engVet Microbiol. 2014 Jul 16;171(3-4):436-40. doi: 10.1016/j.vetmic.2014.02.034. Epub 2014 Mar 30378-113510.1016/j.vetmic.2014.02.034info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:32Zoai:repositorio.insa.pt:10400.18/2956Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:37:54.535327Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antimicrobial resistance determinants in Staphylococcus spp. recovered from birds of prey in Portugal |
title |
Antimicrobial resistance determinants in Staphylococcus spp. recovered from birds of prey in Portugal |
spellingShingle |
Antimicrobial resistance determinants in Staphylococcus spp. recovered from birds of prey in Portugal Sousa, Margarida Resistência aos Antibióticos Coagulase-negative staphylococci Birds of Prey Ica Operon S. aureus S. epidermidis Portugal |
title_short |
Antimicrobial resistance determinants in Staphylococcus spp. recovered from birds of prey in Portugal |
title_full |
Antimicrobial resistance determinants in Staphylococcus spp. recovered from birds of prey in Portugal |
title_fullStr |
Antimicrobial resistance determinants in Staphylococcus spp. recovered from birds of prey in Portugal |
title_full_unstemmed |
Antimicrobial resistance determinants in Staphylococcus spp. recovered from birds of prey in Portugal |
title_sort |
Antimicrobial resistance determinants in Staphylococcus spp. recovered from birds of prey in Portugal |
author |
Sousa, Margarida |
author_facet |
Sousa, Margarida Silva, Nuno Igrejas, Gilberto Silva, Filipe Sargo, Roberto Alegria, Nuno Benito, Daniel Gómez, Paula Lozano, Carmen Gómez-Sanz, Elena Torres, Carmen Caniça, Manuela Poeta, Patrícia |
author_role |
author |
author2 |
Silva, Nuno Igrejas, Gilberto Silva, Filipe Sargo, Roberto Alegria, Nuno Benito, Daniel Gómez, Paula Lozano, Carmen Gómez-Sanz, Elena Torres, Carmen Caniça, Manuela Poeta, Patrícia |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Sousa, Margarida Silva, Nuno Igrejas, Gilberto Silva, Filipe Sargo, Roberto Alegria, Nuno Benito, Daniel Gómez, Paula Lozano, Carmen Gómez-Sanz, Elena Torres, Carmen Caniça, Manuela Poeta, Patrícia |
dc.subject.por.fl_str_mv |
Resistência aos Antibióticos Coagulase-negative staphylococci Birds of Prey Ica Operon S. aureus S. epidermidis Portugal |
topic |
Resistência aos Antibióticos Coagulase-negative staphylococci Birds of Prey Ica Operon S. aureus S. epidermidis Portugal |
description |
Antibiotic resistance among wild animals represent an emerging public health concern. The objective of this study was to analyze the staphylococcal nasal microbiota in birds of prey and their content in antimicrobial resistance determinants. Nasal samples from 16 birds of prey were collected, swabs were dipped and incubated into BHI broth [6.5% NaCl] and later seeded on manitol salt agar and oxacillin-resistance screening agar base media. Staphylococcal colonies were isolated from both media and were identified by biochemical and molecular methods. Susceptibility testing to 18 antimicrobial agents was performed by disk-diffusion method. Six of the 16 tested animals carried staphylococci (37.5%) and 7 isolates of the following species were recovered: Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Staphylococcus sciuri rodentium, Staphylococcus cohnii urealitycum, and Staphylococcus gallinarum. The S. aureus isolate was penicillin-resistant (with blaZ gene) but methicillin-susceptible and was ascribed to spa-type t012, sequence-type ST30 and agr-type III. The S. epidermidis isolate carried blaZ, mecA, mrs(A/B), mphC, tet(K), drfA, and fusC genes, ica operon, and was typed as ST35. The genes ant6′-Ia, tet(K), tet(L), dfrG, cat221, cat194, and cat223 were detected in S. saprophyticus or S. gallinarum isolates. Birds of prey seem to be a natural reservoir of S. aureus and coagulase-negative staphylococci resistant to multiple antibiotics. Due to the convergence between habitats, the contact between wildlife, other animals and humans is now more common and this involves an increased possibility of interchange of these microorganisms in the different ecosystems. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06 2014-06-01T00:00:00Z 2015-02-25T17:00:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/2956 |
url |
http://hdl.handle.net/10400.18/2956 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Vet Microbiol. 2014 Jul 16;171(3-4):436-40. doi: 10.1016/j.vetmic.2014.02.034. Epub 2014 Mar 3 0378-1135 10.1016/j.vetmic.2014.02.034 |
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info:eu-repo/semantics/embargoedAccess |
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embargoedAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
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Elsevier |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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