Multi-layered nanosystems for the controlled release of anticancer drugs
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/25644 |
Resumo: | Cancer is one of the leading causes of death worldwide, and current therapeutic options present numerous drawbacks. Chemotherapy, specifically, is associated with widely known side effects. However, and due to the scientific and technologic advances of the past years, a new approach to this dilemma appears in nanomedicine: the use of nanosystems capable of releasing the anticancer drugs in a controlled way and only at the designated cells. These nanosystems may be obtained from numerous materials, including biopolymers, and by various techniques. Specifically, the work described here consists in the synthesis and characterization of nanoparticles obtained by layer-by-layer assembly from amino-modified spherical SiO2 templates (diameter: 234 ± 19 nm). The alternate deposition of biopolymers - alginate (ALG) and chitosan (CH) or lysozyme nanofibrils (LNFs) - on templates pre-loaded with curcumin allowed the creation of nanoparticles covered in ALG/CH (with a diameter of 243 ± 8 nm) or ALG/LNFs (with 242 ± 8 nm). The systematic reversion the zeta-potential and the observation of the particles by scanning electron microscopy (SEM) confirms the deposition of each biopolymeric layer. The present work also includes the evaluation of the release profile of this drug from the multi-layered nanoparticles, demonstrating a more controlled release than with the bare counterparts. The evaluation of their cytotoxic capacity in human liver cancer cell line (HepG2) was posteriorly evaluated through an MTT assay. The cytotoxic effect of layered particles containing CUR was similar to the unlayered counterparts’, demonstrating that the two layers of biopolymers (ALG/CH or ALG/LNFs) do not compromise their cytotoxic potential. |
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Multi-layered nanosystems for the controlled release of anticancer drugsMulti-layered systemsLayer-by-layer assemblyNanoparticlesNanocarriersBiopolymersChitosanAlginate,Controlled releaseAnticancer drugsCurcuminLysozyme nanofibrilsCancer is one of the leading causes of death worldwide, and current therapeutic options present numerous drawbacks. Chemotherapy, specifically, is associated with widely known side effects. However, and due to the scientific and technologic advances of the past years, a new approach to this dilemma appears in nanomedicine: the use of nanosystems capable of releasing the anticancer drugs in a controlled way and only at the designated cells. These nanosystems may be obtained from numerous materials, including biopolymers, and by various techniques. Specifically, the work described here consists in the synthesis and characterization of nanoparticles obtained by layer-by-layer assembly from amino-modified spherical SiO2 templates (diameter: 234 ± 19 nm). The alternate deposition of biopolymers - alginate (ALG) and chitosan (CH) or lysozyme nanofibrils (LNFs) - on templates pre-loaded with curcumin allowed the creation of nanoparticles covered in ALG/CH (with a diameter of 243 ± 8 nm) or ALG/LNFs (with 242 ± 8 nm). The systematic reversion the zeta-potential and the observation of the particles by scanning electron microscopy (SEM) confirms the deposition of each biopolymeric layer. The present work also includes the evaluation of the release profile of this drug from the multi-layered nanoparticles, demonstrating a more controlled release than with the bare counterparts. The evaluation of their cytotoxic capacity in human liver cancer cell line (HepG2) was posteriorly evaluated through an MTT assay. The cytotoxic effect of layered particles containing CUR was similar to the unlayered counterparts’, demonstrating that the two layers of biopolymers (ALG/CH or ALG/LNFs) do not compromise their cytotoxic potential.O cancro é uma das principais causas de morte no mundo, e as opções terapêuticas atuais apresentam inúmeros inconvenientes. A quimioterapia, especificamente, possui efeitos secundários reconhecidos. No entanto, e graças ao desenvolvimento tecnológico e científico dos últimos anos, surge na nanomedicina uma nova abordagem para este dilema: a utilização de nanossistemas capazes de libertar fármacos anticancerígenos de forma controlada e apenas em células designadas. Estes nanossistemas podem ser obtidos a partir de inúmeros materiais, incluindo biopolímeros, e por variadas técnicas. Neste contexto, o trabalho desenvolvido nesta dissertação consiste na síntese e caracterização de nanopartículas obtidas por montagem camada-a-camada a partir de templates esféricos de SiO2 amino-modificados (diâmetro: 234 ± 19 nm). A deposição alternada de biopolímeros – alginato (ALG) e quitosano (CH) ou nanofibrilas de lisozima (LNFs) - em templates com curcumina previamente incorporada deu origem a nanopartículas cobertas por ALG/CH (com 243 ± 8 nm de diâmetro) ou por ALG/LNFs (com 242 ± 8 nm). A sistemática reversão do potencial-zeta e a observação das partículas por microscopia eletrónica de varrimento (SEM) confirma a deposição de cada camada de biopolímero. O presente trabalho inclui também a avaliação do perfil de libertação do fármaco a partir destas nanopartículas multicamada, demonstrando-se uma libertação mais controlada do que em partículas sem camadas de biopolímeros. A avaliação da capacidade citotóxica em células humanas de cancro do fígado (HepG2) foi posteriormente avaliada através de um ensaio com MTT. O efeito citotóxico das partículas contendo CUR e cobertas de polímeros revelou-se semelhante ao das partículas análogas sem camadas, demonstrando que as duas camadas de biopolímeros (ALG/CH ou ALG/LNFs) não comprometem o potencial citotóxico das mesmas.2021-01-08T00:00:00Z2018-12-20T00:00:00Z2018-12-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/25644TID:202238873engCarvalho, João Pedro Fernandesinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:49:43Zoai:ria.ua.pt:10773/25644Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:58:49.863394Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Multi-layered nanosystems for the controlled release of anticancer drugs |
title |
Multi-layered nanosystems for the controlled release of anticancer drugs |
spellingShingle |
Multi-layered nanosystems for the controlled release of anticancer drugs Carvalho, João Pedro Fernandes Multi-layered systems Layer-by-layer assembly Nanoparticles Nanocarriers Biopolymers Chitosan Alginate, Controlled release Anticancer drugs Curcumin Lysozyme nanofibrils |
title_short |
Multi-layered nanosystems for the controlled release of anticancer drugs |
title_full |
Multi-layered nanosystems for the controlled release of anticancer drugs |
title_fullStr |
Multi-layered nanosystems for the controlled release of anticancer drugs |
title_full_unstemmed |
Multi-layered nanosystems for the controlled release of anticancer drugs |
title_sort |
Multi-layered nanosystems for the controlled release of anticancer drugs |
author |
Carvalho, João Pedro Fernandes |
author_facet |
Carvalho, João Pedro Fernandes |
author_role |
author |
dc.contributor.author.fl_str_mv |
Carvalho, João Pedro Fernandes |
dc.subject.por.fl_str_mv |
Multi-layered systems Layer-by-layer assembly Nanoparticles Nanocarriers Biopolymers Chitosan Alginate, Controlled release Anticancer drugs Curcumin Lysozyme nanofibrils |
topic |
Multi-layered systems Layer-by-layer assembly Nanoparticles Nanocarriers Biopolymers Chitosan Alginate, Controlled release Anticancer drugs Curcumin Lysozyme nanofibrils |
description |
Cancer is one of the leading causes of death worldwide, and current therapeutic options present numerous drawbacks. Chemotherapy, specifically, is associated with widely known side effects. However, and due to the scientific and technologic advances of the past years, a new approach to this dilemma appears in nanomedicine: the use of nanosystems capable of releasing the anticancer drugs in a controlled way and only at the designated cells. These nanosystems may be obtained from numerous materials, including biopolymers, and by various techniques. Specifically, the work described here consists in the synthesis and characterization of nanoparticles obtained by layer-by-layer assembly from amino-modified spherical SiO2 templates (diameter: 234 ± 19 nm). The alternate deposition of biopolymers - alginate (ALG) and chitosan (CH) or lysozyme nanofibrils (LNFs) - on templates pre-loaded with curcumin allowed the creation of nanoparticles covered in ALG/CH (with a diameter of 243 ± 8 nm) or ALG/LNFs (with 242 ± 8 nm). The systematic reversion the zeta-potential and the observation of the particles by scanning electron microscopy (SEM) confirms the deposition of each biopolymeric layer. The present work also includes the evaluation of the release profile of this drug from the multi-layered nanoparticles, demonstrating a more controlled release than with the bare counterparts. The evaluation of their cytotoxic capacity in human liver cancer cell line (HepG2) was posteriorly evaluated through an MTT assay. The cytotoxic effect of layered particles containing CUR was similar to the unlayered counterparts’, demonstrating that the two layers of biopolymers (ALG/CH or ALG/LNFs) do not compromise their cytotoxic potential. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-20T00:00:00Z 2018-12-20 2021-01-08T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/25644 TID:202238873 |
url |
http://hdl.handle.net/10773/25644 |
identifier_str_mv |
TID:202238873 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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