Ammonia impact on the selection of a phototrophic - chemotrophic consortium for polyhydroxyalkanoates production under light-feast / dark-aerated-famine conditions
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/162658 |
Resumo: | Research Unit on Applied Molecular Biosciences - UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB. Likewise, the INCOVER project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement n° 689242 ). J.R.A. also acknowledges the financial support of FCT - Fundação para a Ciência e a Tecnologia through the Ph.D. grant DFA/BD/8201/2020 . Publisher Copyright: © 2023 The Authors |
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Ammonia impact on the selection of a phototrophic - chemotrophic consortium for polyhydroxyalkanoates production under light-feast / dark-aerated-famine conditionsFeast and famine selectionOutdoor simulated-conditionsPhototrophic-chemotrophic consortiumPolyhydroxyalkanoatesUncoupled ammonia-carbon feedingWaste valorisationEnvironmental EngineeringCivil and Structural EngineeringEcological ModellingWater Science and TechnologyWaste Management and DisposalPollutionSDG 6 - Clean Water and SanitationResearch Unit on Applied Molecular Biosciences - UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB. Likewise, the INCOVER project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement n° 689242 ). J.R.A. also acknowledges the financial support of FCT - Fundação para a Ciência e a Tecnologia through the Ph.D. grant DFA/BD/8201/2020 . Publisher Copyright: © 2023 The AuthorsPhototrophic polyhydroxyalkanoate (PHA) production is an emerging technology for recovering carbon and nutrients from diverse wastewater streams. However, reliable selection methods for the enrichment of PHA accumulating purple phototrophic bacteria (PPB) in phototrophic mixed cultures (PMC) are needed. This research evaluates the impact of ammonia on the selection of a PHA accumulating phototrophic-chemotrophic consortium, towards the enrichment of PHA accumulating PPB. The culture was operated under light-feast/dark-aerated-famine and winter simulated-outdoor conditions (13.2 ± 0.9 °C, transient light, 143.5 W/m2), using real fermented domestic wastewater with molasses as feedstock. Three ammonia supply strategies were assessed: 1) ammonia available only in the light phase, 2) ammonia always present and 3) ammonia available only during the dark-aerated-famine phase. Results showed that the PMC selected under 1) ammonia only in the light and 3) dark-famine ammonia conditions, presented the lowest PHA accumulation capacity during the light period (11.1 % g PHA/g VSS and 10.4 % g PHA/g VSS, respectively). In case 1), the absence of ammonia during the dark-aerated-famine phase did not promote the selection of PHA storing PPB, whereas in case 3) the absence of ammonia during the light period favoured cyanobacteria growth as well as purple sulphur bacteria with increased non-PHA inclusions, resulting in an overall decrease of phototrophic PHA accumulation capacity. The best PHA accumulation performance was obtained with selection under permanent presence of ammonia (case 2), which attained a PHA content of 21.6 % g PHA/g VSS (10.2 Cmmol PHA/L), at a production rate of 0.57 g PHA/L·day, during the light period in the selection reactor. Results in case 2 also showed that feedstock composition impacts the PMC performance, with feedstocks richer in more reduced volatile fatty acids (butyric and valeric acids) decreasing phototrophic performance and leading to acids entering the dark-aerated phase. Nevertheless, the presence of organic carbon in the aerated phase was not detrimental to the system. In fact, it led to the establishment of a phototrophic-chemotrophic consortium that could photosynthetically accumulate a PHA content of 13.2 % g PHA/g VSS (6.7 Cmmol PHA/L) at a production rate of 0.20 g PHA/L·day in the light phase, and was able to further increase that storage up to 18.5 % g PHA/g VSS (11.0 Cmmol PHA/L) at a production rate of 1.35 g PHA/L·day in the dark-aerated period. Furthermore, the light-feast/dark-aerated-famine operation was able to maintain the performance of the selection reactor under winter conditions, unlike non-aerated PMC systems operated under summer conditions, suggesting that night-time aeration coupled with the constant presence of ammonia can contribute to overcoming the seasonal constraints of outdoor operation of PMCs for PHA production.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNAlmeida, J. R.León, E. SerranoCorona, E. LaraFradinho, J. C.Oehmen, A.Reis, M. A. M.2024-01-22T23:00:01Z2023-10-012023-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article13application/pdfhttp://hdl.handle.net/10362/162658eng0043-1354PURE: 82176469https://doi.org/10.1016/j.watres.2023.120450info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:45:36Zoai:run.unl.pt:10362/162658Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:59:00.576471Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Ammonia impact on the selection of a phototrophic - chemotrophic consortium for polyhydroxyalkanoates production under light-feast / dark-aerated-famine conditions |
| title |
Ammonia impact on the selection of a phototrophic - chemotrophic consortium for polyhydroxyalkanoates production under light-feast / dark-aerated-famine conditions |
| spellingShingle |
Ammonia impact on the selection of a phototrophic - chemotrophic consortium for polyhydroxyalkanoates production under light-feast / dark-aerated-famine conditions Almeida, J. R. Feast and famine selection Outdoor simulated-conditions Phototrophic-chemotrophic consortium Polyhydroxyalkanoates Uncoupled ammonia-carbon feeding Waste valorisation Environmental Engineering Civil and Structural Engineering Ecological Modelling Water Science and Technology Waste Management and Disposal Pollution SDG 6 - Clean Water and Sanitation |
| title_short |
Ammonia impact on the selection of a phototrophic - chemotrophic consortium for polyhydroxyalkanoates production under light-feast / dark-aerated-famine conditions |
| title_full |
Ammonia impact on the selection of a phototrophic - chemotrophic consortium for polyhydroxyalkanoates production under light-feast / dark-aerated-famine conditions |
| title_fullStr |
Ammonia impact on the selection of a phototrophic - chemotrophic consortium for polyhydroxyalkanoates production under light-feast / dark-aerated-famine conditions |
| title_full_unstemmed |
Ammonia impact on the selection of a phototrophic - chemotrophic consortium for polyhydroxyalkanoates production under light-feast / dark-aerated-famine conditions |
| title_sort |
Ammonia impact on the selection of a phototrophic - chemotrophic consortium for polyhydroxyalkanoates production under light-feast / dark-aerated-famine conditions |
| author |
Almeida, J. R. |
| author_facet |
Almeida, J. R. León, E. Serrano Corona, E. Lara Fradinho, J. C. Oehmen, A. Reis, M. A. M. |
| author_role |
author |
| author2 |
León, E. Serrano Corona, E. Lara Fradinho, J. C. Oehmen, A. Reis, M. A. M. |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
UCIBIO - Applied Molecular Biosciences Unit DQ - Departamento de Química RUN |
| dc.contributor.author.fl_str_mv |
Almeida, J. R. León, E. Serrano Corona, E. Lara Fradinho, J. C. Oehmen, A. Reis, M. A. M. |
| dc.subject.por.fl_str_mv |
Feast and famine selection Outdoor simulated-conditions Phototrophic-chemotrophic consortium Polyhydroxyalkanoates Uncoupled ammonia-carbon feeding Waste valorisation Environmental Engineering Civil and Structural Engineering Ecological Modelling Water Science and Technology Waste Management and Disposal Pollution SDG 6 - Clean Water and Sanitation |
| topic |
Feast and famine selection Outdoor simulated-conditions Phototrophic-chemotrophic consortium Polyhydroxyalkanoates Uncoupled ammonia-carbon feeding Waste valorisation Environmental Engineering Civil and Structural Engineering Ecological Modelling Water Science and Technology Waste Management and Disposal Pollution SDG 6 - Clean Water and Sanitation |
| description |
Research Unit on Applied Molecular Biosciences - UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB. Likewise, the INCOVER project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement n° 689242 ). J.R.A. also acknowledges the financial support of FCT - Fundação para a Ciência e a Tecnologia through the Ph.D. grant DFA/BD/8201/2020 . Publisher Copyright: © 2023 The Authors |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-10-01 2023-10-01T00:00:00Z 2024-01-22T23:00:01Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/162658 |
| url |
http://hdl.handle.net/10362/162658 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
0043-1354 PURE: 82176469 https://doi.org/10.1016/j.watres.2023.120450 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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13 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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