Polyhydroxyalkanoates production in purple phototrophic bacteria ponds

Detalhes bibliográficos
Autor(a) principal: Almeida, J. R.
Data de Publicação: 2024
Outros Autores: León, E. Serrano, Rogalla, F., Fradinho, J. C., Oehmen, A., Reis, M. A. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/163062
Resumo: This work was supported by national funds from FCT - Fundação para a Ciência e a Tecnologia , I.P., in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences - UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB . Likewise, the INCOVER project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement n° 689242 ). J.R.A. also acknowledges the financial support of FCT - Fundação para a Ciência e a Tecnologia through the Ph.D. grant DFA/BD/8201/2020. Publisher Copyright: © 2023 The Author(s)
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spelling Polyhydroxyalkanoates production in purple phototrophic bacteria pondsA breakthrough in outdoor pilot-scale operationOutdoor operationPilot-scalePolyhydroxyalkanoate (PHA)Purple phototrophic bacteria ponds (PPB ponds)Resource recoveryUp-flow anaerobic sludge blanket reactor (UASB)Environmental EngineeringEnvironmental ChemistryWaste Management and DisposalPollutionSDG 6 - Clean Water and SanitationSDG 9 - Industry, Innovation, and InfrastructureThis work was supported by national funds from FCT - Fundação para a Ciência e a Tecnologia , I.P., in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences - UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB . Likewise, the INCOVER project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement n° 689242 ). J.R.A. also acknowledges the financial support of FCT - Fundação para a Ciência e a Tecnologia through the Ph.D. grant DFA/BD/8201/2020. Publisher Copyright: © 2023 The Author(s)The versatile capacity of purple phototrophic bacteria (PPB) for producing valuable bioproducts has gathered renewed interest in the field of resource recovery and waste valorisation. However, greater knowledge regarding the viability of applying PPB technologies in outdoor, large-scale systems is required. This study assessed, for the first time, the upscaling of the phototrophic polyhydroxyalkanoate (PHA) production technology in a pilot-scale system operated in outdoor conditions. An integrated system composed of two up-flow anaerobic sludge blanket (UASB) reactors (for fermentation of wastewater with molasses), and two high-rate algal ponds retrofitted into PPB ponds, was operated in a wastewater treatment plant under outdoor conditions. UASB's adaptation to the outdoor temperatures involved testing different operational settings, namely hydraulic retention times (HRT) of 48 and 72 h, and molasses fermentation in one or two UASBs. Results have shown that the fermentation of molasses in both UASBs with an increased HRT of 72 h was able to ensure a suitable operation during colder conditions, achieving 3.83 ± 0.63 g CODFermentative Products/L, compared to the 3.73 ± 0.85 g CODFermentative Products/L achieved during warmer conditions (molasses fermentation in one UASB; HRT 48 h). Furthermore, the PPB ponds were operated under a light-feast/dark-aerated-famine strategy and fed with the fermented wastewater and molasses from the two UASBs. The best PHA production was obtained during the summer of 2018 and spring of 2019, attaining 34.7 % gPHA/gVSS with a productivity of 0.11 gPHA L−1 day−1 and 36 % gPHA/gVSS with a productivity of 0.14 gPHA L−1 day−1, respectively. Overall, this study showcases the first translation of phototrophic PHA production technology from an artificially illuminated laboratory scale system into a naturally illuminated, outdoor, pilot-scale system. It also addresses relevant process integration aspects with UASBs for pre-fermenting wastewater with molasses, providing a novel operational strategy to achieve photosynthetic PHA production in outdoor full-scale systems.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNAlmeida, J. R.León, E. SerranoRogalla, F.Fradinho, J. C.Oehmen, A.Reis, M. A. M.2024-02-02T23:41:18Z2024-02-202024-02-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://hdl.handle.net/10362/163062eng0048-9697PURE: 82176756https://doi.org/10.1016/j.scitotenv.2023.168899info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:46:09Zoai:run.unl.pt:10362/163062Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:59:15.198945Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Polyhydroxyalkanoates production in purple phototrophic bacteria ponds
A breakthrough in outdoor pilot-scale operation
title Polyhydroxyalkanoates production in purple phototrophic bacteria ponds
spellingShingle Polyhydroxyalkanoates production in purple phototrophic bacteria ponds
Almeida, J. R.
Outdoor operation
Pilot-scale
Polyhydroxyalkanoate (PHA)
Purple phototrophic bacteria ponds (PPB ponds)
Resource recovery
Up-flow anaerobic sludge blanket reactor (UASB)
Environmental Engineering
Environmental Chemistry
Waste Management and Disposal
Pollution
SDG 6 - Clean Water and Sanitation
SDG 9 - Industry, Innovation, and Infrastructure
title_short Polyhydroxyalkanoates production in purple phototrophic bacteria ponds
title_full Polyhydroxyalkanoates production in purple phototrophic bacteria ponds
title_fullStr Polyhydroxyalkanoates production in purple phototrophic bacteria ponds
title_full_unstemmed Polyhydroxyalkanoates production in purple phototrophic bacteria ponds
title_sort Polyhydroxyalkanoates production in purple phototrophic bacteria ponds
author Almeida, J. R.
author_facet Almeida, J. R.
León, E. Serrano
Rogalla, F.
Fradinho, J. C.
Oehmen, A.
Reis, M. A. M.
author_role author
author2 León, E. Serrano
Rogalla, F.
Fradinho, J. C.
Oehmen, A.
Reis, M. A. M.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Almeida, J. R.
León, E. Serrano
Rogalla, F.
Fradinho, J. C.
Oehmen, A.
Reis, M. A. M.
dc.subject.por.fl_str_mv Outdoor operation
Pilot-scale
Polyhydroxyalkanoate (PHA)
Purple phototrophic bacteria ponds (PPB ponds)
Resource recovery
Up-flow anaerobic sludge blanket reactor (UASB)
Environmental Engineering
Environmental Chemistry
Waste Management and Disposal
Pollution
SDG 6 - Clean Water and Sanitation
SDG 9 - Industry, Innovation, and Infrastructure
topic Outdoor operation
Pilot-scale
Polyhydroxyalkanoate (PHA)
Purple phototrophic bacteria ponds (PPB ponds)
Resource recovery
Up-flow anaerobic sludge blanket reactor (UASB)
Environmental Engineering
Environmental Chemistry
Waste Management and Disposal
Pollution
SDG 6 - Clean Water and Sanitation
SDG 9 - Industry, Innovation, and Infrastructure
description This work was supported by national funds from FCT - Fundação para a Ciência e a Tecnologia , I.P., in the scope of the project UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences - UCIBIO and the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB . Likewise, the INCOVER project has received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreement n° 689242 ). J.R.A. also acknowledges the financial support of FCT - Fundação para a Ciência e a Tecnologia through the Ph.D. grant DFA/BD/8201/2020. Publisher Copyright: © 2023 The Author(s)
publishDate 2024
dc.date.none.fl_str_mv 2024-02-02T23:41:18Z
2024-02-20
2024-02-20T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/163062
url http://hdl.handle.net/10362/163062
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0048-9697
PURE: 82176756
https://doi.org/10.1016/j.scitotenv.2023.168899
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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